SLC7A5 (NM_003486) Human Untagged Clone

SKU SC124075

SLC7A5 (untagged)-Human solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 (SLC7A5)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SLC7A5
• Synonyms: 4F2LC; CD98; D16S469E; E16; LAT1; MPE16
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124075 sequence for NM_003486 edited (data generated by NextGen Sequencing); ATGGCGGGTGCGGGCCCGAAGCGGCGCGCGCTAGCGGCGCCGGCGGCCGAGGAGAAGGAA; GAGGCGCGGGAGAAGATGCTGGCCGCCAAGAGCGCGGACGGCTCGGCGCCGGCAGGCGAG; GGCGAGGGCGTGACCCTGCAGCGGAACATCACGCTGCTCAACGGCGTGGCCATCATCGTG; GGGACCATTATCGGCTCGGGCATCTTCGTGACGCCCACGGGCGTGCTCAAGGAGGCAGGC; TCGCCGGGGCTGGCGCTGGTGGTGTGGGCCGCGTGCGGCGTCTTCTCCATCGTGGGCGCG; CTCTGCTACGCGGAGCTCGGCACCACCATCTCCAAATCGGGCGGCGACTACGCCTACATG; CTGGAGGTCTACGGCTCGCTGCCCGCCTTCCTCAAGCTCTGGATCGAGCTGCTCATCATC; CGGCCTTCATCGCAGTACATCGTGGCCCTGGTCTTCGCCACCTACCTGCTCAAGCCGCTC; TTCCCCACCTGCCCGGTGCCCGAGGAGGCAGCCAAGCTCGTGGCCTGCCTCTGCGTGCTG; CTGCTCACGGCCGTGAACTGCTACAGCGTGAAGGCCGCCACCCGGGTCCAGGATGCCTTT; GCCGCCGCCAAGCTCCTGGCCCTGGCCCTGATCATCCTGCTGGGCTTCGTCCAGATCGGG; AAGGGTGATGTGTCCAATCTAGATCCCAACTTCTCATTTGAAGGCACCAAACTGGATGTG; GGGAACATTGTGCTGGCATTATACAGCGGCCTCTTTGCCTATGGAGGATGGAATTACTTG; AATTTCGTCACAGAGGAAATGATCAACCCCTACAGAAACCTGCCCCTGGCCATCATCATC; TCCCTGCCCATCGTGACGCTGGTGTACGTGCTGACCAACCTGGCCTACTTCACCACCCTG; TCCACCGAGCAGATGCTGTCGTCCGAGGCCGTGGCCGTGGACTTCGGGAACTATCACCTG; GGCGTCATGTCCTGGATCATCCCCGTCTTCGTGGGCCTGTCCTGCTTCGGCTCCGTCAAT; GGGTCCCTGTTCACATCCTCCAGGCTCTTCTTCGTGGGGTCCCGGGAAGGCCACCTGCCC; TCCATCCTCTCCATGATCCACCCACAGCTCCTCACCCCCGTGCCGTCCCTCGTGTTCACG; TGTGTGATGACGCTGCTCTACGCCTTCTCCAAGGACATCTTCTCCGTCATCAACTTCTTC; AGCTTCTTCAACTGGCTCTGCGTGGCCCTGGCCATCATCGGCATGATCTGGCTGCGCCAC; AGAAAGCCTGAGCTTGAGCGGCCCATCAAGGTGAACCTGGCCCTGCCTGTGTTCTTCATC; CTGGCCTGCCTCTTCCTGATCGCCGTCTCCTTCTGGAAGACACCCGTGGAGTGTGGCATC; GGCTTCACCATCATCCTCAGCGGGCTGCCCGTCTACTTCTTCGGGGTCTGGTGGAAAAAC; AAGCCCAAGTGGCTCCTCCAGGGCATCTTCTCCACGACCGTCCTGTGTCAGAAGCTCATG; CAGGTGGTCCCCCAGGAGACATAG; ; Clone variation with respect to NM_003486.5;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003486 unedited; ATTGTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGCGCACAC; TGCTCGCTGGGCCGCGGCTCCCGGGTGTCCCAGGCCCGGCCGGTGCGCAGAGCATGGCGG; GTGCGGGCCCGAAGCGGCGCGCGCTAGCGGCGCCGGCGGCCGAGGAGAAGGAAGAGGCGC; GGGAGAAGATGCTGGCCGCCAAGAGCGCGGACGGCTCGGCGCCGGCAGGCGAGGGCGAGG; GCGTGACCCTGCAGCGGAACATCACGCTGCTCAACGGCGTGGCCATCATCGTGGGGACCA; TTATCGGCTCGGGCATCTTCGTGACGCCCACGGGCGTGCTCAAGGAGGCAGGCTCGCCGG; GGCTGGCGCTGGTGGTGTGGGCCGCGTGCGGCGTCTTCTCCATCGTGGGCGCGCTCTGCT; ACGCGGAGCTCGGCACCACCATCTCCAAATCGGGCGGCGACTACGCCTACATGCTGGAGG; TCTACGGCTCGCTGCCCGCCTTCCTCAAGCTCTGGATCGAGCTGCTCATCATCCGGCCTT; CATCGCAGTACATCGTGGCCCTGGTCTTCGCCACCTACCTGCTCAAGCCGCTCTTCCCCA; CCTGCCCGGTGCCCGAGGAGGCAGCCAAGCTCGTGGCCTGCCTCGGGTGCTGCTGTCACG; CCGTGACTGGTCGCGTGNGGCGCACCGGTCAGATGCTTGCGCGCNACTCTGCCTGGCTGT; ATCTGTGGCCGTCAATCGGAGGTGGTCACTAATCACTTCATGAGCCACGGTGGACTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003486 unedited; CATGGCAACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGA; TCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACCCAAAATTTAAGTTTAAAAAAAATT; TTTTTTTTAGTTAACAAAGGGGGGAAGGGAAAAAGGGCCCCAGGGGACCAAAAGCTGCTC; CTGGGCAGGAGCACAGGCACACCTGGGTCCCTGGCCCTCAGTTGAGGGATGAGATTCGTA; CCAAAGTTTTTACAGCAGCCTCCACTGCCCGACCTGGGAGGGACCGCCAGGGACTGGGAT; GGGCAACTTAACTGTGGGTTGCGGGGAACCCGAGTGGGTTCGAGGAGGTGATCTACTTTA; ACTGGGCTCTGGGCATGCTCCTCCGGGAGCCACTCGGCCTCCTGGCTATGTCTCCTGGGG; GACCACCTGCATGAGCTTCTGACACAAGACGGTCGTGGAGAAAATGCCCTGGAGGAGCCA; CTTGGGCTTGTTTTTCCACCAGACCCCGAAGAAGTAGACGGGCACCCCGCTTAGGATGAT; GGTGAAACCCATGCCACACTCCACGGGTGTCTTCCAGAAGGAGACGGCGATTAGGAAGAG; GCAGGCCAGGATTAAAAACACAGGCCGGGGCCAGGTTCACCCTTGATGGGGCCGTTAAAG; CTTAGGGCTTTTTGTGGCGCAACCCAATTTATGCCCATTATGGGCCCAGGCCCCCCCAAA; GACCCTTTTAAAAACCCTTTAAAAATTTTATTAACGGGGAAATTTTCCTTGGGGAAAGCT; AAACAACCTTCT;
• Restriction Sites: Please inquire
• ACCN: NM_003486
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003486.5, NP_003477.4
• RefSeq Size: 4543 bp
• RefSeq ORF: 1524 bp
• Locus ID: 8140
• UniProt ID: Q01650
• Cytogenetics: 16q24.2
• Domains: aa_permeases
• Protein Families: Druggable Genome, Transmembrane
• Summary: The heterodimer with SLC3A2 functions as sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, L-DOPA, leucine, histidine, methionine and tryptophan (PubMed:9751058, PubMed:10049700, PubMed:11557028, PubMed:10391915, PubMed:10574970, PubMed:11311135, PubMed:11564694, PubMed:12117417, PubMed:12225859, PubMed:25998567, PubMed:30867591). Functions as an amino acid exchanger (PubMed:11557028, PubMed:12117417, PubMed:12225859, PubMed:30867591). May play a role in the transport of L-DOPA across the blood-brain barrier (By similarity). May act as the major transporter of tyrosine in fibroblasts (Probable). May mediate blood-to-retina L-leucine transport across the inner blood-retinal barrier (By similarity). Can mediate the transport of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane (PubMed:11564694, PubMed:12225859). When associated with LAPTM4B, the heterodimer formed by SLC3A2 and SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation (PubMed:25998567). Involved in the uptake of toxic methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes (PubMed:12117417). Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the membrane (PubMed:15769744).[UniProtKB/Swiss-Prot Function]