IRS1 (NM_005544) Human Untagged Clone
$1,082.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | IRS1 |
| Synonyms | HIRS-1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_005544 |
| Insert Size | 5000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005544.1, NP_005535.1 |
| RefSeq Size | 5828 bp |
| RefSeq ORF | 3729 bp |
| Locus ID | 3667 |
| UniProt ID | P35568 |
| Cytogenetics | 2q36.3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus |
| Summary | This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. provided by RefSeq, Nov 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005544" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208765 | IRS1 (Myc-DDK-tagged)-Human insulin receptor substrate 1 (IRS1) | 10 ug |
$589.00
$1,081.00
|
|
| RC208765L1 | Lenti-ORF clone of IRS1 (Myc-DDK-tagged)-Human insulin receptor substrate 1 (IRS1) | 10 ug |
$1,381.00
|
|
| RC208765L2 | Lenti-ORF clone of IRS1 (mGFP-tagged)-Human insulin receptor substrate 1 (IRS1) | 10 ug |
$1,381.00
|
|
| RC208765L3 | Lenti-ORF clone of IRS1 (Myc-DDK-tagged)-Human insulin receptor substrate 1 (IRS1) | 10 ug |
$1,381.00
|
|
| RC208765L4 | Lenti-ORF clone of IRS1 (mGFP-tagged)-Human insulin receptor substrate 1 (IRS1) | 10 ug |
$1,381.00
|
|
| RG208765 | IRS1 (tGFP-tagged) - Human insulin receptor substrate 1 (IRS1) | 10 ug |
$789.00
$1,281.00
|
|
Citations
MicroRNA-126 induces autophagy by altering cell metabolism in malignant mesothelioma
Tomasetti, Monaco, Manzella et al
Oncotarget (2016) 7 (24), 36338-36352 DOI: 10.18632/oncotarget.8916
Tomasetti, Monaco, Manzella et al
Oncotarget (2016) 7 (24), 36338-36352 DOI: 10.18632/oncotarget.8916
Cited by 47
AI Summary: OriGene products, including the pCMV-MiR and pRS empty plasmids, MIR126 expression plasmid, and human IRS1 overexpression plasmid (SC124032), were used to manipulate MIR126 and IRS1 expression in malignant mesothelioma cells, enabling the study of their roles in autophagy and cell metabolism.
Snippet: Cells (2×105) were stably transfected with 1 μg of the pCMV-MiR and/or pRS (OriGene) empty plasmid or with plasmid carrying the MIR126 sequence 5′-UCG UAC CGU GAG UAA UAA UGC G-3′ (OriGene, Rockville, MD) and/or shRNA pRS plasmid, with the HIF1α-targeting sequence of 5′-ACA AGA ACC TAC TGC TAA TGC CAC CAC TA-3′, and with the acetyl-CoA citrate lyase (ACL)-targeting sequence of 5′-CCA TCA CTG AGG TCT TCA AGG AAG AGA TG-3′, using the TransIT-LT1 reagent (Mirus). Anti-IRS1 sequence 5′-AGA CCA UCA GCU UCG UGA ATT-3′ (Ambion), or human IRS1 plasmid (SC124032, OriGene), were used to transiently silence or overexpress IRS1, respectively
The tyrosine phosphatase PTPN14 (Pez) inhibits metastasis by altering protein trafficking
Belle, Ali, Lonic et al
Sci Signal (2015) 8 (364), ra18 DOI: 10.1126/scisignal.2005547
Belle, Ali, Lonic et al
Sci Signal (2015) 8 (364), ra18 DOI: 10.1126/scisignal.2005547
Cited by 80
AI Summary: In this study, OriGene products—specifically, PTPN14 shRNA lentiviral particles (TL313968) and PTPN14 cDNA (RC207890)—were used to manipulate PTPN14 expression in breast cancer cells, enabling the authors to investigate how PTPN14 regulates protein trafficking and metastasis.
Oleanolic acid supplement attenuates liquid fructose-induced adipose tissue insulin resistance through the insulin receptor substrate-1/phosphatidylinositol 3-kinase/Akt signaling pathway in rats
Li, Wang, Gu et al
Toxicol. Appl. Pharmacol. (2014) 277 (2), 155-163 DOI: 10.1016/j.taap.2014.03.016
Li, Wang, Gu et al
Toxicol. Appl. Pharmacol. (2014) 277 (2), 155-163 DOI: 10.1016/j.taap.2014.03.016
Cited by 54
AI Summary: In this study, OriGene’s IRS-1 antibody was used to assess protein expression changes in adipose tissue, helping demonstrate that oleanolic acid ameliorates fructose-induced insulin resistance in rats via the IRS-1/PI3K/Akt signaling pathway.
Snippet: Insulin receptor substrate (IRS)-1 (Origene Technologies, Inc., Rockville, MD, USA), Phosphorylated IRS-1 at serine-307 (pIRS-1), Phosphorylated Akt at serine-473 (pAkt) and Akt (Santa Cruz Biotechnology, Santa Cruz, CA, USA) were detected with rabbit polyclonal ... Cite SaveSaving
Gene expression of the IGF pathway family distinguishes subsets of gastrointestinal stromal tumors wild type for KIT and PDGFRA
Beadling, Patterson, Justusson et al
Cancer Med (2013) 2 (1), 21-31 DOI: 10.1002/cam4.57
Beadling, Patterson, Justusson et al
Cancer Med (2013) 2 (1), 21-31 DOI: 10.1002/cam4.57
Cited by 30
AI Summary: OriGene products, specifically the IGF1R (NM_000875), IGF1 (NM_000618), IGF2 (NM_000612), and INSR (NM_000208) qPCR primer sets, were used to measure gene expression levels of key IGF pathway members in gastrointestinal stromal tumor samples, enabling the identification of distinct wild-type GIST subtypes based on their molecular profiles.
Showing 1-4 of 4 papers.
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