PGAM5 (NM_138575) Human Untagged Clone

SKU SC123147

PGAM5 (untagged)-Human phosphoglycerate mutase family member 5 (PGAM5), nuclear gene encoding mitochondrial protein, transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: PGAM5
• Synonyms: BXLBV68
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_138575 edited; GCGCGGGCCGGCGCGGGAGCAAGCGGCATGGCGTTCCGGCAGGCGCTGCAGCTGGCGGCC; TGCGGGCTGGCCGGGGGCTCGGCCGCCGTGCTCTTCTCGGCCGTGGCGGTAGGGAAGCCG; CGCGCAGGCGGGGACGCGGAGCCACGCCCGGCTGAGCCGCCGGCCTGGGCGGGGGGCGCG; CGGCCGGGCCCCGGTGTCTGGGACCCCAACTGGGACAGGCGAGAACCACTGTCTCTGATC; AACGTGCGGAAGAGGAACGTGGAATCTGGGGAAGAAGAGCTGGCGTCCAAGCTGGACCAC; TACAAAGCCAAGGCCACGCGGCACATCTTCCTCATCAGGCATTCCCAGTACCACGTGGAT; GGCTCCCTGGAGAAGGACCGCACTCTGACCCCGCTGGGTCGGGAGCAGGCTGAACTCACT; GGGCTCCGCCTGGCAAGCTTGGGGTTGAAGTTTAATAAAATCGTCCATTCGTCTATGACG; CGCGCCATAGAGACCACCGATATCATCAGCCGGCACCTGCCAGGCGTCTGCAAAGTCAGC; ACAGATCTGCTGCGGGAAGGCGCCCCCATCGAGCCAGACCCGCCCGTGTCTCATTGGAAG; CCGGAAGCTGTGCAGTATTACGAAGACGGAGCCCGGATCGAGGCCGCCTTCCGGAACTAC; ATCCACCGCGCAGATGCCAGGCAGGAGGAGGACAGTTACGAAATCTTCATCTGTCACGCC; AACGTCATCCGCTACATCGTGTGCAGCATCCCGCCGCTGTTGTCCGCTGGGGATTTTGTG; GTTCTGGGGTCCTGACCTCTTTCACTTGCTGATCTGTGGGCGCTCCCACCCGTGTGCCAG; CGTGACGGCTCGGGGTGTCCGCTCCCCTCTGGGTCGAGGCCACAGCTGAGTCACGTTGCT; GCTCGGGCTGCTCCCTCGGGGGGCCCTTGTCCCTCAACCTGCTCTGGTGCCCCACTCTCA; GCACCACAGAATGATCCGGGTTCAGGTTGCGTTTTCCCTGCCACCACCCTGCAATCAGCC; ACTTCTTTAAGGAGCTCCAGGGCTGCAGCCACGTTAGAGGGCCCCTTGGGGGGCAGGGCC; AGCTCTACGGTTACATGCCTGAAACAGTCAGAAGGGTTGGCCAAATCTCACTAAATGCAA; ATTTGAAACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138575 unedited; NTTGTTCGAATTTTGTAATACGACTTCACTATAGGGCGGCCGCGATTCCCGGGATATCGT; CGACCCACGCGTCCGGCGCGGGCCGGCGCGGGAGCAAGCGGCATGGCGTTCCGGCAGGCG; CTGCAGCTGGCGGCCTGCGGGCTGGCCGGGGGCTCGGCCGCCGTGCTCTTCTCGGCCGTG; GCGGTAGGGAAGCCGCGCGCAGGCGGGGACGCGGAGCCACGCCCGGCTGAGCCGCCGGCC; TGGGCGGGGGGCGCGCGGCCGGGCCCCGGTGTCTGGGACCCCAACTGGGACAGGCGAGAA; CCACTGTCTCTGATCAACGTGCGGAAGAGGAACGTGGAATCTGGGGAAGAAGAGCTGGCG; TCCAAGCTGGACCACTACAAAGCCAAGGCCACGCGGCACATCTTCCTCATCAGGCATTCC; CAGTACCACGTGGATGGCTCCCTGGAGAAGGACCGCACTCTGACCCCGCTGGGTCGGGAG; CAGGCTGAACTCACTGGGCTCCGCCTGGCAAGCTTGGGGTTGAAGTTTAATAAAATCGTC; CATTCGTCTATGACGCGCGCCATAGAGACCACCGATATCATCAGCCGGCACCTGCCAGGC; GTCTGCAAAGTCAGCACAGATCTGCTGCGGGAAGGCGCCCCCATCGAGCCAGACCCGCCC; GTGTCTCATTGGAAGCCGGAAGCTGTGCAGTATTACGAAGACGGAGCCCGGATCGAGGCC; GCCTTCCGGAACTACATCCACCGCGCAGATGCCAGGCAGGAGGAGGACAGTTACGAAATC; TTCATCTGTCACGCCAACGTCATCCGCTACATCGTGTGCAGCATCCCGCCGCTGTTGTCC; GCTGGGNGATTTTGTGGTTCTGGGGTCCTGACCTCTTTCACTTGCTGATCTGTGGGCGCT; CCCACCCGTGTGCCAGCGTGC;
• Restriction Sites: Please inquire
• ACCN: NM_138575
• Insert Size: 1214 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138575.1, NP_612642.1
• RefSeq Size: 1214 bp
• RefSeq ORF: 768 bp
• Locus ID: 192111
• UniProt ID: Q96HS1
• Cytogenetics: 12q24.33
• Protein Families: Transmembrane
• Summary: Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (3) differs in the 3' UTR and lacks a portion of the 3' coding region compared to variant 1. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1.