FA2H (NM_024306) Human Untagged Clone

SKU SC122956

FA2H (untagged)-Human fatty acid 2-hydroxylase (FA2H)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FA2H
• Synonyms: FAAH; FAH1; FAXDC1; SCS7; SPG35
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024306 edited; AGCAGACGCCGGGCTGCGCGCTGGGAGCTGCGGTCTCCGGCCATGGCCCCCGCTCCGCCC; CCCGCCGCCTCCTTCTCGCCCTCCGAGGTCCAGCGGCGCCTGGCGGCCGGCGCGTGCTGG; GTCCGCCGCGGGGCCCGCCTCTACGACCTCTCCAGCTTCGTGCGGCACCACCCGGGGGGC; GAGCAGCTGCTGCGGGCCAGGGCGGGCCAGGACATCAGCGCCGACCTGGACGGGCCGCCG; CACAGGCACTCGGCCAACGCGCGCCGCTGGCTGGAGCAGTACTACGTGGGAGAGCTCCGC; GGGGAGCAGCAGGGCTCCATGGAGAACGAGCCTGTAGCCCTTGAGGAAACTCAGAAGACA; GATCCTGCTATGGAACCACGGTTCAAAGTGGTGGATTGGGACAAGGACCTGGTGGACTGG; CGAAAGCCTCTCCTGTGGCAGGTGGGCCACTTGGGAGAGAAGTACGATGAGTGGGTTCAC; CAGCCGGTGACCAGGCCCATCCGCCTCTTCCACTCAGACCTCATTGAGGGCCTCTCTAAG; ACTGTCTGGTACAGTGTCCCCATCATCTGGGTGCCCCTGGTGCTGTATCTCAGCTGGTCC; TACTACCGAACCTTTGCCCAGGGCAACGTCCGACTCTTCACGTCATTTACAACAGAGTAC; ACGGTGGCAGTGCCCAAGTCCATGTTCCCCGGGCTCTTCATGCTGGGGACATTCCTCTGG; AGCCTCATCGAGTACCTCATCCACCGCTTCCTGTTCCACATGAAGCCCCCCAGCGACAGC; TATTACCTCATCATGCTGCACTTCGTCATGCACGGCCAGCACCACAAGGCACCCTTCGAC; GGCTCCCGCCTGGTCTTCCCCCCTGTGCCAGCCTCCCTGGTGATCGGCGTCTTCTACTTG; TGCATGCAGCTCATCCTGCCTGAGGCAGTAGGGGGCACTGTGTTTGCGGGGGGCCTCCTG; GGCTACGTCCTCTATGACATGACCCATTACTACCTGCACTTTGGCTCGCCGCACAAGGGC; TCCTACCTGTACAGCCTGAAGGCCCACCACGTCAAGCACCACTTTGCACATCAGAAGTCA; GGATTTGGTATCAGCACTAAATTGTGGGATTACTGTTTCCACACCCTCACTCCAGAGAAA; CCCCACCTGAAGACGCAGTGACAACTCCCACCCCCTCCGTCCTGCCCTCAGCCCGGCCCT; GGCCCCTTCCCGACCCCCACCCGCCATTCAGACCCCATTAAGAAGGTTGGCTTGGCCAGG; CAGGATGGGCTGTGTCCGGCCCTGCAGCCTAGTGGAAGGTGCTGAGGGGGCCCTGAGGCA; GGACCGCCCTCCTGACCCCTGGTAGGAGGGTCACATCCACTTGGTGGCCAGGTGGCCCTT; GGTGACCCACTTCTTCCTGGAGCGTCCCTGCCTAGAGCTCAGCCCACAGGACTGCTTCAG; GCCGTGGCCACAGGTAGCAGCCGCAAGGGGAAATGAAGAAAACTGAGCCCTCGTGGCCAC; CTGTGTCACCCTTGTGCCTTAGCCTCATGGGCTGCCTAGGAGCTGCCTGCACGGCACAGC; TCGCTTTCACAGTCAGAAGTGGGTCTGTGGGATCTGTGGTCCCTGTCCTCCCTGCTGTCC; CTTCTGGGGAGGCTTTGGTGGCTCTGAGGTGGACAAAGAGCTCTCGCAAGAAGAGACAGC; GTGATGCCTCCCACAGTCCACCCCAGACCCTGGGGCAGCCCCTCTGGCCCTGCCAGCTGC; CTGCGTCGTTGGGCCCAGGGTGGCTGGCAGGAGTCCCAGCTGCTTGCTTTAGGACCTCGC; AGCTTTTCTTGCCGTCCCTCCCCTGCCTCCAGAATCACAGCCCTTCTCCCCAAGGGAGGC; TGAGGAGGCTTCTCCACCAGTGGCAGCCCCACCCCGTCCCTGGCCATTCTTGGCCTCCAC; CCCGCTCAGGCCCCTACTCGGGCGCTCCCAGAAGGAGCCACCTCTCAGTGCCTCACCTCC; CCCTGCCTCCCAGCCTCCGCAGATGAGGTTCCTGCCCCTTCCTCCTCGTAACCAAAACCC; TCACTGCTCCCAGGACGGTCTTATTTATAAACCAGATACATGTTCTTAGTCTGGTCCCAG; ACCAAGGAGCTGGTCAGACGGCCCTTTCTAATCCTACATGTTGAGCTTATGTAAAAAATG; TTGTTTCCTCCTGTTTTTGGTTCCTTTCTTACCCACAAACCATTACTACTTGAAACTTAA; AAAACTCGCCAAGTGTAAAGGCTAAAGAGAAGCAGTTTGACGGACCTTGTGATTTGTACT; GTTTGCTGCGGAGCTATTTAAAGATTTTGGAATAAATATACAAAACTACGGTTGTGAAAT; AAAAACTTAAATTGTATATTTTGAAAAATAAAACACTGAAAAGAAACCAAAAAAAAAAAA; AAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024306 unedited; NAACGTCAGAATTGTATACGACTCATATAGGCGGCCGCNGAATTCGCACGAGGAGCAGAC; GCCGGGCTGCGCGCTGGGAGCTGCGGTCTCCGGCCATGGCCCCCGCTCCGCCCCCCGCCG; CCTCCTTCTCGCCCTCCGAGGTCAAGCGGCGCCTGGCGGCCGGCGCGTGCTGGGTCCGCC; GCGGGGCCCGCCTCTACGACCTCTCCAGCTTCGTGCGGCACCACCCGGGGGGCGAGCAGC; TGCTGCGGGCCAGGGCGGGCCAGGACATCAGCGCCGACCTGGACGGGCCGCCGCACAGGC; ACTCGGCCAACGCGCGCCGCTGGCTGGAGCAGTACTACGTGGGAGAGCTCCGCGGGGAGC; AGCAGGGCTCCATGGAGAACGAGCCTGTAGCCCTTGAGGAAACTCAGAAGACAGATCCTG; CTATGGAACCACGGTTCAAAGTGGTGGATTGGGACAAGGACCTGGTGGACTGGCGAAAGC; CTCTCCTGTGGCAGGTGGGCCACTTGGGAGAGAAGTACGATGAGTGGGTTCACCAGCCGG; TGACCAGGCCCATCCGCCTCTTCCACTCAGACCTCATTGAGGGCCTCTCTAAGACTGTCT; GGTACAGTGTCCCCATCATCTGGGTGCCCCTGGTGCTGTATCTCAGCTGGTCCTACTACC; GAACCTTTGCCCAGGGCAACGTCCGACTCTTCACGTCATTTACAACAGAGTACACGGTGG; CAGTGCCCAAGTCCATGTTCCCCGGGCTCTTCATGCTGGGGACATTCCTCTGGAGCCTCA; TCGAGTACCTCATCCACCGCTTCCTGNTCCACATGAAGCCCCCCAGCGACAGCTATTACC; TCATCATGCTGCACTTCGTCATGCACGGCCAGCACCACAAGGCACCCTTNCGACGCTCCC; GNCTGGTCTTCCCCCCTGTC;
• Restriction Sites: Please inquire
• ACCN: NM_024306
• Insert Size: 2416 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024306.2, NP_077282.2
• RefSeq Size: 2419 bp
• RefSeq ORF: 1119 bp
• Locus ID: 79152
• UniProt ID: Q7L5A8
• Cytogenetics: 16q23.1
• Protein Families: Transmembrane
• Summary: This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.[provided by RefSeq, Mar 2010]