PDF (NM_022341) Human Untagged Clone

SKU SC122932

PDF (untagged)-Human peptide deformylase (mitochondrial) (PDF), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: PDF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022341 edited; CATGGCCCGGCTGTGGGGCGCGCTGAGTCTTCGGCCACTGTGGGCGGCCGTGCCGTGGGG; CGGGGCGGCAGCCGTCGGTGTCCGGGCTTGCAGCTCCACGGCCGCCCCGGACGGCGTCGA; GGGCCCGGCGCTGCGGCGCTCCTATTGGCGCCACCTGAGGCGTCTGGTGCTGGGTCCTCC; CGAACCGCCGTTCTCGCACGTGTGCCAAGTCGGGGACCCGGTGCTGCGCGGCGTGGCGGC; CCCGGTGGAGCGGGCGCAGCTAGGCGGGCCCGAGCTGCAGCGGCTGACGCAACGGCTGGT; CCAGGTGATGCGGCGGCGGCGCTGCGTGGGCCTAAGCGCGCCGCAGCTGGGGGTGCCGCG; GCAGGTGCTGGCGCTGGAGCTCCCCGAGGCGCTGTGTCGGGAGTGCCCGCCCCGCCAGCG; CGCGCTCCGCCAAATGGAGCCCTTCCCCCTGCGCGTGTTCGTGAACCCCAGCCTGCGAGT; GCTTGACAGCCGCCTGGTCACCTTTCCCGAGGGCTGCGAGAGCGTCGCCGGCTTCCTGGC; CTGCGTGCCCCGCTTCCAGGCGGTGCAGATCTCAGGGCTGGACCCCAATGGAGAACAGGT; GGTGTGGCAGGCGAGCGGGTGGGCAGCCCGCATCATCCAGCACGAGATGGACCACCTGCA; GGGCTGCCTGTTTATTGACAAAATGGACAGCAGGACGTTCACAAACGTCTATTGGATGAA; GGTGAATGACTAAAGCTTTGCTACTGGGGCTGAGGATTCCGGATACCAAGACGCAAACAC; TTTCACTTTGAGCTGGGCAAATCTTACTTGGCATCAACTTGGATGGCTCGCATATGACAG; GAAACTGGATTGCCAAGGCATGGCAGACTGAGCTGGAGGAAGATGTCAGAAATGTTTGCC; CTGAAATCAGTTACGGACAAAATGTTGGCTACAACTTGAGGAGAAAAATCACCCCCAAAG; GAGTGGACATTTCCTAACAATTCTGTATGGAGGAAGGTGGGGTAATTGCATTCGTCTGCA; GTAGACACGAGTTCCTCGGACCTGTATAATCTCCCAAAGCCAAGGTTTGGTATTAATGTA; GTCCCCAAATACCTGAAAGCTGTCTTTAAAAATGCAGGTAAGCATGTGACTGGCAAAAAA; AAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022341 unedited; GGGCCGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATCCCGGGATATCGTCG; ACCCACGCGTCCGCATGGCCCGGCTGTGGGGCGCGCTGAGTCTTCGGCCACTGTGGGCGG; CCGTGCCGTGGGGCGGGGCGGCAGCCGTCGGTGTCCGGGCTTGCAGCTCCACGGCCGCCC; CGGACGGCGTCGAGGGCCCGGCGCTGCGGCGCTCCTATTGGCGCCACCTGAGGCGTCTGG; TGCTGGGTCCTCCCGAACCGCCGTTCTCGCACGTGTGCCAAGTCGGGGACCCGGTGCTGC; GCGGCGTGGCGGCCCCGGTGGAGCGGGCGCAGCTAGGCGGGCCCGAGCTGCAGCGGCTGA; CGCAACGGCTGGTCCAGGTGATGCGGCGGCGGCGCTGCGTGGGCCTAAGCGCGCCGCAGC; TGGGGGTGCCGCGGCAGGTGCTGGCGCTGGAGCTCCCCGAGGCGCTGTGTCGGGAGTGCC; CGCCCCGCCAGCGCGCGCTCCGCCAAATGGAGCCCTTCCCCCTGCGCGTGTTCGTGAACC; CCAGCCTGCGAGTGCTTGACAGCCGCCTGGTCACCTTTCCCGAGGGCTGCGAGAGCGTCG; CCGGCTTCCTGGCCTGCGTGCCCCGCTTCCAGGCGGTGCAGATCTCAGGGCTGGACCCCA; ATGGAGAACAGGTGGTGTGGCANGCGAGCGGGTGGGCAGCCCGCATCATCCAGCACGAGA; TGGACCACCTGCNAGGCTGCCTGTTTATTGANCAAATGGACAGCAGGACGTTCACAAACG; TCTATTGGATGAAGGTGAATGACTAAAGCTTTGCTACTGGGGCTNGAGATTCCGGATACC; AAGACGCAACACTTTCACTTTGAGCTGGGCAATCTTACTTGGCATCAACTGGGATG;
• Restriction Sites: Please inquire
• ACCN: NM_022341
• Insert Size: 1156 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022341.1, NP_071736.1
• RefSeq Size: 1180 bp
• RefSeq ORF: 732 bp
• Locus ID: 64146
• UniProt ID: Q9HBH1
• Cytogenetics: 16q22.1
• Summary: Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq, Nov 2008]