LIME (LIME1) (NM_017806) Human Untagged Clone

SKU SC122866

LIME1 (untagged)-Human Lck interacting transmembrane adaptor 1 (LIME1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: LIME
• Synonyms: dJ583P15.4; LIME
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017806 edited; CCCCTGGCCGTGGGCTTGGGCCTCGGCTTCACAGGATGGGGCTGCCAGTGTCCTGGGCCC; CTCCTGCCCTCTGGGTTCTAGGGTGCTGCGCCCTGCTCCTCTCGCTGTGGGCGCTGTGCA; CAGCCTGCCGCAGGCCCGAGGACGCTGTAGCCCCCAGGAAGAGGGCGCGGAGGCAGCGGG; CGAGGCTGCAGGGCAGTGCGACGGCGGCGGAAGCGTCCCTACTGAGGCGGACCCACCTCT; GCTCCCTCAGCAAGTCGGACACCAGACTGCACGAGCTGCACCGGGGCCCGCGCAGCAGCA; GGGCCCTGCGGCCTGCCAGCATGGATCTCCTGCGCCCACACTGGCTGGAGGTGTCCAGGG; ACATCACCGGACCGCAGGCAGCCCCCTCTGCCTTCCCACACCAGGAGCTGCCCCGGGCTC; TGCCGGCAGCTGCAGCCACCGCAGGGTGCGCTGGCCTCGAGGCCACCTATTCCAACGTGG; GGCTGGCGGCCCTTCCCGGGGTCAGCCTGGCGGCCAGCCCTGTGGTGGCCGAGTATGCCC; GCGTCCAGAAGCGCAAAGGGACCCATCGCAGTCCCCAAGAGCCACAGCAGGGGAAGACTG; AGGTGACCCCGGCCGCTCAGGTGGACGTCCTGTACTCCAGGGTCTGCAAGCCTAAAAGGA; GGGACCCAGGACCCACCACAGACCCGCTGGACCCCAAGGGCCAGGGAGCGATTCTGGCCC; TGGCGGGTGACCTGGCCTACCAGACCCTCCCGCTCAGGGCCCTGGATGTGGACAGCGGCC; CCCTGGAAAACGTGTATGAGAGCATCCGGGAGCTGGGGGACCCTGCTGGCAGGAGCAGCA; CGTGCGGGGCTGGGACGCCCCCTGCTTCCAGCTGCCCCAGCCTAGGGAGGGGCTGGAGAC; CCCTCCCTGCCTCCCTGCCCTGAACACTCAAGGACCTGTGCTCCTTCCTCCAGAGTGAGG; CCCGTCCCCCGCCCCGCCCCGCCTCACAGCTGACAGCGCCAGTCCCAGGTCCCCGGGCCG; CCAGCCCGTGAGGTCCGTGAGGTCCTGGCCGCTCTGACAGCCGCGGCCTCCCCGGGCTCC; AGAGAAGGCCCGCGTCTAAATAAAGCGCCAGCGCAGGATGAAAGCGAAAAAAAAAAAAAA; A;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017806 unedited; TCACTATTAGGGGCGGCCGCGNAATTCCCGGGGTATCGTCGACCCACGCGTCCGCCCCTG; GCCGTGGGCTTGGGCCTCGGCTTCACAGGATGGGGCTGCCAGTGTCCTGGGCCCCTCCTG; CCCTCTGGGTTCTAGGGTGCTGCGCCCTGCTCCTCTCGCTGTGGGCGCTGTGCACAGCCT; GCCGCATGCCCGAGGACGCTGTAGCCCCCATGAAGAGGGCGCGGAGGCAGCGGGCGATGC; TGCAGGGCAGTGCGACGGCGGCGGAAGCGTCCCTACTGAGGCGGACCCACCTCTGCTCCC; TCAGCAAGTCGGACACCAGACTGCACGAGCTGCACCGGGGCCCGCGCAGCAGCAGGGCCC; TGCGGCCTGCCAGCATGGATCTCCTGCGCCCACACTGGCTGGAGGTGTCCAGGGACATCA; CCGGACCGCATGCAGCCCCCTCTGCCTTCCCACACCAGGAGCTGCCCCGGGCTCTGCCGG; CAGCTGCAGCCACCGCAGGGTGCGCTGGCCTCGAGGCCACCTATTCCAACGTGGGGCTGG; CGGCCCTTCCCGGGGTCAGCCTGGCGGCCAGCCCTGTGGTGGCCGAGTATGCCCGCGTCC; AGAAGCGCAAAGGGACCCATCGCAGTCCCCAAGAGCCACAGCAGGGGAAGACTGAGGTGA; CCCCGGCCGCTCATGTGGACGTCCTGTACTCCAGGGTCTGCAAGCCTAAAAGGAGGGACC; CAGGACCCACCACAGACCCGCTGGACCCCAAGGGCCAGGGAGCCGATTCTGGCCCTGGCG; GGTGACCTGGCCTACCANACCCTCCCGCTCATGGCCCTGGTTGTGGACAGCGGCCCCCTG; GAAAACGTGTATGAGAGCATCCCG;
• Restriction Sites: Please inquire
• ACCN: NM_017806
• Insert Size: 1141 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017806.1, NP_060276.1
• RefSeq Size: 1196 bp
• RefSeq ORF: 888 bp
• Locus ID: 54923
• UniProt ID: Q9H400
• Cytogenetics: 20q13.33
• Protein Families: Transmembrane
• Summary: This gene encodes a transmembrane adaptor protein that links the T and B-cell receptor stimulation to downstream signaling pathways via its association with the Src family kinases Lck and Lyn, respectively. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2015]; Transcript Variant: This variant (1) encodes the longest isoform (1).