TTLL12 (NM_015140) Human Untagged Clone

SKU SC122822

TTLL12 (untagged)-Human tubulin tyrosine ligase-like family, member 12 (TTLL12)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TTLL12
• Synonyms: dJ526I14.2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015140 edited; CTGGAGTCGGCGCGGGTGCTGGCGCCATGGAGGCCGAGCGGGGTCCCGAGCGCCGGCCTG; CGGAGCGTAGCAGCCCGGGCCAGACGCCGGAGGAGGGCGCGCAGGCCTTGGCCGAGTTCG; CGGCGCTGCACGGCCCGGCGCTGCGCGCTTCGGGGGTCCCCGAACGTTACTGGGGCCGCC; TCCTGCACAAGCTGGAGCACGAGGTTTTCGACGCTGGGGAAGTGTTTGGGATCATGCAAG; TGGAGGAGGTAGAAGAGGAGGAGGACGAGGCAGCCCGGGAGGTGCGGAAGCAGCAGCCCA; ACCCGGGGAACGAGCTGTGCTACAAGGTCATCGTGACCAGGGAGAGCGGGCTCCAGGCAG; CCCACCCCAACAGCATCTTCCTCATCGACCACGCCTGGACGTGCCGTGTGGAGCACGCGC; GCCAGCAGCTGCAGCAGGTGCCCGGGCTGCTGCACCGCATGGCCAACCTGATGGGCATTG; AGTTCCACGGTGAGCTGCCCAGTACAGAGGCTGTGGCCCTGGTGCTGGAGGAGATGTGGA; AGTTCAACCAGACCTACCAGCTGGCCCATGGGACAGCTGAGGAGAAGATGCCGGTGTGGT; ATATCATGGACGAGTTCGGTTCGCGGATCCAGCACGCGGACGTGCCCAGCTTCGCCACGG; CACCCTTCTTCTACATGCCGCAGCAGGTGGCCTACACGCTGCTGTGGCCCCTGAGGGACC; TGGACACTGGCGAGGAGGTGACCCGAGACTTTGCCTACGGAGAGACGGACCCCCTGATCC; GGAAGTGCATGCTGCTGCCCTGGGCCCCCACCGACATGCTGGACCTCAGCTCTTGCACAC; CCGAGCCGCCCGCCGAGCACTACCAGGCCATTCTGGAGGAAAACAAGGAGAAGCTGCCAC; TTGACATCAACCCCGTGGTGCACCCCCACGGCCACATCTTCAAGGTCTACACGGACGTGC; AGCAGGTGGCCAGCAGCCTCACCCACCCGCGCTTCACCCTCACCCAGAGTGAGGCGGACG; CCGACATCCTCTTCAACTTCTCACACTTCAAGGACTACAGGAAACTCAGCCAGGAGAGGC; CAGGCGTGCTGCTGAACCAGTTCCCCTGCGAGAACCTGCTGACTGTCAAGGACTGCCTGG; CCTCCATCGCGCGCCGGGCAGGTGGCCCCGAGGGCCCACCCTGGCTGCCCCGAACCTTCA; ACCTGCGCACTGAGCTGCCCCAGTTTGTCAGCTACTTCCAGCAGCGGGAAAGGTGGGGCG; AGGACAACCACTGGATCTGCAAGCCCTGGAACCTGGCGCGCAGCCTGGACACCCACGTCA; CCAAGAGCCTGCACAGCATCATCCGGCACCGAGAGAGCACCCCCAAGGTTGTGTCCAAGT; ACATCGAAAGTCCCGTGTTGTTCCTTCGAGAAGACGTGGGAAAGGTCAAGTTCGACATCC; GCTACATCGTGCTGCTGCGGTCAGTGAGGCCCCTACGGTTGTTCGTGTATGATGTGTTCT; GGCTGCGGTTCTCCAACCGGGCCTTTGCACTCAACGACCTGGATGACTACGAGAAGCACT; TCACGGTCATGAACTATGACCCGGATGTGGTGCTGAAGCAGGTGCACTGTGAAGAGTTCA; TCCCCGAGTTTGAGAAGCAATACCCAGAATTTCCCTGGACGGACGTCCAGGCTGAGATCT; TCCGGGCCTTCACGGAGCTGTTCCAGGTGGCCTGTGCCAAGCCACCACCCCTGGGCCTCT; GCGACTACCCCTCATCCCGGGCCATGTATGCCGTCGACCTCATGCTGAAGTGGGACAACG; GCCCAGATGGAAGGCGGGTGATGCAGCCGCAGATCCTGGAGGTGAACTTCAACCCCGACT; GTGAGCGAGCCTGCAGGTACCACCCCACCTTCTTCAACGACGTCTTCAGCACCTTGTTTC; TGGACCAGCCCGGTGGCTGCCACGTTACCTGCCTTGTCTAGGCACTCGCTGTCCCCAAAA; CCTGTGCTTGGGGCAGGATTCCAACCTCAGTTCTCTGAGCTGCTTCTGCAAAGGCCCCCA; TGTCCCTCCCCACACCGGCCCTGGGCATAGCCTCAGCCCCAGGCCTCTGTCCTGCCGAGC; CATCCTCCCGGCGCCACACTCCGGGAGCACAGCATCCTCCTCTCACCTGTGGGTCAGAGC; AGGACAGTGATGGTGTCCCCAGGGCTGAGCACCACCCCACGCCCTGCCCTCACCCCTCAC; CACCATCTGTGCACTGATGAGTCTCCAGTTTAGCCAAGGGCTTCGTTCCTGGCATGGAGA; ATTTGTTCCTGGCTGCTGTGTTTCCAGGGGGTGCTGGGGGAAGGGTTCCGTGGAGCGAGA; CAAGGTGTCCTCGGGAGCAGGGTTCCACCGGGAAGCGTTTGGGAGCCCTGTATCACACGG; GGCAGGCGGGTTTCTCTTCCGGGGTCTCTGCTCTTATGCATCAGGACGACCCCGGGACGG; CTGTGGGGCCCCACACTGCACCCACAGGGCTCTATGCGACAGGGGCCCAGGAACAGCCTG; AGGCCACCACCCAGCAAGCCCGCCTTATCACCCATTCCAGCTCACCCAGAACCTTCACCA; GCAAACCTCCTGCTGAGGTCCTGGCAGGAGGCCACCGTCTTGTTACCGTTTCCTTTTCGT; TTGCTGAGGGTCACAGACCCCAACAGGGAAATCAGTATCTGTCTTCCCAGTGGTTGCCCT; GCTCGCCGGGCACTCCACGGGGTCCCGCCCTTGTGTGAGATGGGCCAGGATCCTTCGGCA; AGGGGCGCCTGGGGCTGGGGCTGATTGTGGGCGGTGGAGCGCCAGACAGAAAAGGATTCC; AATGAGAACTTCAGGTTAAAGTCAGATGCCACCTACCAGGGTCTACAGTCAAAATGTTGG; CTTTTTCTTATTTTTTAATGTATGGGAGAAAAATGTAAAATTCCAGTTCTTTTCTAATTG; TGTTTCTGAAATTAGGAGTCAGCTGCCAGCGTTTTTGTGTGGCTGCAGTGTGCCTGGGCC; CAGCTCACGGGCAGTGGGTGGACCTAACTGCCCAGGCAGGCGAGAGCTACTTCCAGAGCC; TTCCAGTGCATGGGAGGGCAGGGCTAGGTGTAGCGGTGTCTCCTCTTTGAAATTAAGAAC; TATCTTTCTTGTAGCAAAGCTGCACCTGATGATGCTGCCTCTCCTCTCTGTGTTGTCTGG; GCCCTTGTTTACAAGCACGCGTTACCCTTCCTGAGGGGAGCCATGCTCTAGCCCCTGGAG; GGCCTGTTGCAGGGGCAGGGCGGGCCCGTCGCCTTTGGCAGCTCCTGGAGAGCTGTGGAC; ATGCAGTCCCCCTCAGTTCGTGCTGCAATAAAGGCCATCTTCTCTTAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015140 unedited; ATGTCATTTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTGGAGTCG; GCGCGGGTGCTGGCGCCATGGAGGCCGAGCGGGGTCCCGAGCGCCGGCCTGCGGAGCGTA; GCATCCCGGGCCAGACGCCGGAGGAGGGCGCGCAGGCCTTGGCCGAGTTCGCGGCGCTGC; ACGGCCCGGCGCTGCGCGCTTCGGGGGTCCCCGAACGTTACTGGGGCCGCCTCCTGCACA; AGCTGGAGCACGAGGTTTTCGACGCTGGGGAAGTGTTTGGGATCATGCAAGTGGAGGAGG; TAGAAGAGGAGGAGGACGAGGCAGCCCGGGAGGTGCGGAAGCAGCAGCCCAACCCGGGGA; ACGAGCTGTGCTACAAGGTCATCGTGACCAGGGAGAGCGGGCTCCAGGCAGCCCACCCCA; ACAGCATCTTCCTCATCGACCACGCCTGGACGTGCCGTGTGGAGCACGCGCGCCAGCAGC; TGCAGCATGTGCCCGGGCTGCTGCACCGCATGGCCAACCTGATGGGCATTGAGTTCCACG; GTGAGCTGCCCAGTACAGAGGCTGTGGCCCTGGTGCTGGAGGAGATGTGGAAGTTCAACC; AGACCTACCAGCTGGCCCATGGGACAGCTGAGGAGAAGATGCCGGTGTGGTATATCATGG; ACGAGTTCGGTTCGCGGATCCAGCACGCGGACGTGCCCAGCTTCGCCACGGCACCCTTCT; TCTACATGCCGCATCATGTGGCCTACACGCTGCTGTGGCCCCTGAGGGACCTGGACACTG; GCGAGGAGGTGACCCGAGACTTTGCCTACGGAGAGACCGACCCCCTGATCCGGAAGTGCA; TGCTGGTGCCCTGGGCCCCCACCGAATGCTGGACCTCAGCTCTTGCCACCCGAGCG;
• Restriction Sites: Please inquire
• ACCN: NM_015140
• Insert Size: 3394 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015140.2, NP_055955.1
• RefSeq Size: 3353 bp
• RefSeq ORF: 1935 bp
• Locus ID: 23170
• UniProt ID: Q14166
• Cytogenetics: 22q13.2
• Summary: Negatively regulates post-translational modifications of tubulin, including detyrosination of the C-terminus and polyglutamylation of glutamate residues (PubMed:20162578, PubMed:23251473). Also, indirectly promotes histone H4 trimethylation at 'Lys-20' (H4K20me3) (PubMed:23251473). Probably by controlling tubulin and/or histone H4 post-translational modifications, plays a role in mitosis and in maintaining chromosome number stability (PubMed:20162578, PubMed:23251473). During RNA virus-mediated infection, acts as a negative regulator of the DDX58/RIG-I pathway by preventing MAVS binding to TBK1 and IKBKE (PubMed:28011935).[UniProtKB/Swiss-Prot Function]