HSD17B8 (NM_014234) Human Untagged Clone

SKU SC122798

HSD17B8 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 8 (HSD17B8)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HSD17B8
• Synonyms: D6S2245E; dJ1033B10.9; FABG; FABGL; H2-KE6; HKE6; KE6; RING2; SDR30C1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014234 edited; GGGATTCCCACCCACCCACAGCCCGCCATGGCGTCTCAGCTCCAGAACCGACTCCGCTCC; GCACTGGCCTTGGTCACAGGTGCGGGGAGCGGCATCGGCCGAGCGGTCAGTGTACGCCTG; GCCGGAGAGGGGGCCACCGTAGCTGCCTGCGACCTGGACCGGGCAGCGGCACAGGAGACG; GTGCGGCTGCTGGGCGGGCCAGGGAGCAAGGAGGGGCCGCCCCGAGGGAACCATGCTGCC; TTCCAGGCTGACGTGTCTGAGGCCAGGGCCGCCAGGTGCCTGCTGGAACAAGTGCAGGCC; TGCTTTTCTCGCCCACCATCTGTCGTTGTGTCCTGTGCGGGCATCACCCAGGATGAGTTT; CTGCTGCACATGTCTGAGGATGACTGGGACAAAGTCATAGCTGTCAACCTCAAGGGCACC; TTCCTAGTCACTCAGGCTGCAGCACAAGCCCTGGTGTCCAATGGTTGTCGTGGTTCCATC; ATCAACATCAGTAGCATCGTAGGAAAGGTGGGGAACGTGGGGCAGACAAACTATGCAGCA; TCCAAGGCTGGAGTGATTGGGCTGACCCAGACCGCAGCCCGGGAGCTTGGACGACATGGG; ATCCGCTGTAACTCTGTCCTCCCAGGGTTCATTGCAACACCCATGACACAGAAAGTGCCA; CAGAAAGTGGTGGACAAGATTACTGAAATGATCCCGATGGGACACTTGGGGGACCCTGAG; GATGTGGCAGATGTGGTCGCATTCTTGGCATCTGAAGATAGTGGATACATCACAGGGACC; TCAGTGGAAGTCACTGGAGGTCTTTTCATGTAACTGCCTCAAGGACCCTGGACTCTGCTC; ACCCCCCCACCACTCTGCCTGGCCTCCTGCTGATGAGGACTCTAAGTTCCCAGGATACAA; AAGGGGTGGCAGTGTATGGTTCAGGAATGCTGAATATGGGAAGCAGGGGTGCTTGTGACC; CTAATAAATTCCAAGTCCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014234 unedited; GTCAGCATTTGTAATACGACTCACTATAGGGCGGCACGCGATTCCCGGGATATCGTCGAC; CCACGTCGTCCGGGGATTCCCACCCACCCACAGCCCGCCATGGCGTCTCAGCTCCAGAAC; CGACTCCGCTCCGCACTGGCCTTGGTCACAGGTGCGGGGAGCGGCATCGGCCGAGCGGTC; AGTGTACGCCTGGCCGGAGAGGGGGCCACCGTAGCTGCCTGCGACCTGGACCGGGCAGCG; GCACAGGAGACGGTGCGGCTGCTGGGCGGGCCATGGAGCAAGGAGGGGCCGCCCCGAGGG; AACCATGCTGCCTTCCAGGCTGACGTGTCTGAGGCCAGGGCCGCCAGGTGCCTGCTGGAA; CAAGTGCAAGCCTGCTTTTCTCGCCCACCATCTGTCGTTGTGTCCTGTGCGGGCATCACC; CAGGATGAGTTTCTGCTGCACATGTCTGANGATGACTGGGACAAAGTCATAGCTGTCAAC; CTCAAGGGCACCTTCCTAGTCACTCAAGCTGCAGCACAAGCCCTGGTGTCCAATGGTTGT; CGTGGTTCCATCATCAACATCAGTAGCATCGTAGGANAGGTGGGGAACGTGGGGCAGACA; NACTATGCAGCATCCAAGGCTGGAGTGATTGGGCTGACCCANACCGCAGCCCGGGAGCTT; GGACGACATGGGATCCGCTGTAACTCTGTCCTCCCAGGGTTCATTGCAACACCCATGACA; CAGAAAGTGCCACAGAAAGTGGTGGACAAGATTACTGAAATGATCCCGATGGGACACTTG; GGGGACCCCTGAGATGTGGCANATGTGGTCGCATTCTTGGCATCTGAAGATAGTGGATAC; ATCACAGGGACCTCANTGGGAGTCACTGGNAGTCCTTTCATGTNACTGCCTCAAGGACCC; TGGACTCTGCTTACCCCCCACACTCTGCCTGGCTCCTGCTGATAC;
• Restriction Sites: Please inquire
• ACCN: NM_014234
• Insert Size: 1039 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014234.3, NP_055049.1
• RefSeq Size: 989 bp
• RefSeq ORF: 786 bp
• Locus ID: 7923
• UniProt ID: Q92506
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways
• Summary: In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]