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PRAME (NM_006115) Human Untagged Clone

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SKU
SC122737
PRAME (untagged)-Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1
  $475.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PRAME
Synonyms CT130; MAPE; OIP-4; OIP4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_006115 edited
GGGAAACCGACTCCTGGGAGCAGGGAGGAACGCGCGCTCCAGAGACAACTTCGCGGTGTG
GTGAACTCTCTGAGGAAAAACACGTGCGTGGCAACAAGTGACTGAGACCTAGAAATCCAA
GCGTTGGAGGTCCTGAGGCCAGCCTAAGTCGCTTCAAAATGGAACGAAGGCGTTTGTGGG
GTTCCATTCAGAGCCGATACATCAGCATGAGTGTGTGGACAAGCCCACGGAGACTTGTGG
AGCTGGCAGGGCAGAGCCTGCTGAAGGATGAGGCCCTGGCCATTGCCGCCCTGGAGTTGC
TGCCCAGGGAGCTCTTCCCGCCACTCTTCATGGCAGCCTTTGACGGGAGACACAGCCAGA
CCCTGAAGGCAATGGTGCAGGCCTGGCCCTTCACCTGCCTCCCTCTGGGAGTGCTGATGA
AGGGACAACATCTTCACCTGGAGACCTTCAAAGCTGTGCTTGATGGACTTGATGTGCTCC
TTGCCCAGGAGGTTCGCCCCAGGAGGTGGAAACTTCAAGTGCTGGATTTACGGAAGAACT
CTCATCAGGACTTCTGGACTGTATGGTCTGGAAACAGGGCCAGTCTGTACTCATTTCCAG
AGCCAGAAGCAGCTCAGCCCATGACAAAGAAGCGAAAAGTAGATGGTTTGAGCACAGAGG
CAGAGCAGCCCTTCATTCCAGTAGAGGTGCTCGTAGACCTGTTCCTCAAGGAAGGTGCCT
GTGATGAATTGTTCTCCTACCTCATTGAGAAAGTGAAGCGAAAGAAAAATGTACTACGCC
TGTGCTGTAAGAAGCTGAAGATTTTTGCAATGCCCATGCAGGATATCAAGATGATCCTGA
AAATGGTGCAGCTGGACTCTATTGAAGATTTGGAAGTGACTTGTACCTGGAAGCTACCCA
CCTTGGCGAAATTTTCTCCTTACCTGGGCCAGATGATTAATCTGCGTAGACTCCTCCTCT
CCCACATCCATGCATCTTCCTACATTTCCCCGGAGAAGGAAGAGCAGTATATCGCCCAGT
TCACCTCTCAGTTCCTCAGTCTGCAGTGCCTGCAGGCTCTCTATGTGGACTCTTTATTTT
TCCTTAGAGGCCGCCTGGATCAGTTGCTCAGGCACGTGATGAACCCCTTGGAAACCCTCT
CAATAACTAACTGCCGGCTTTCGGAAGGGGATGTGATGCATCTGTCCCAGAGTCCCAGCG
TCAGTCAGCTAAGTGTCCTGAGTCTAAGTGGGGTCATGCTGACCGATGTAAGTCCCGAGC
CCCTCCAAGCTCTGCTGGAGAGAGCCTCTGCCACCCTCCAGGACCTGGTCTTTGATGAGT
GTGGGATCACGGATGATCAGCTCCTTGCCCTCCTGCCTTCCCTGAGCCACTGCTCCCAGC
TTACAACCTTAAGCTTCTACGGGAATTCCATCTCCATATCTGCCTTGCAGAGTCTCCTGC
AGCACCTCATCGGGCTGAGCAATCTGACCCACGTGCTGTATCCTGTCCCCCTGGAGAGTT
ATGAGGACATCCATGGTACCCTCCACCTGGAGAGGCTTGCCTATCTGCATGCCAGGCTCA
GGGAGTTGCTGTGTGAGTTGGGGCGGCCCAGCATGGTCTGGCTTAGTGCCAACCCCTGTC
CTCACTGTGGGGACAGAACCTTCTATGACCCGGAGCCCATCCTGTGCCCCTGTTTCATGC
CTAACTAGCTGGGTGCACATATCAAATGCTTCATTCTGCATACTTGGACACTAAAGCCAG
GATGTGCATGCATCTTGAAGCAACAAAGCAGCCACAGTTTCAGACAAATGTTCAGTGTGA
GTGAGGAAAACATGTTCAGTGAGGAAAAAACATTCAGACAAATGTTCAGTGAGGAAAAAA
AGGGGAAGTTGGGGATAGGCAGATGTTGACTTGAGGAGTTAATGTGATCTTTGGGGAGAT
ACATCTTATAGAGTTAGAAATAGAATCTGAATTTCTAAAGGGAGATTCTGGCTTGGGAAG
TACATGTAGGAGTTAATCCCTGTGTAGACTGTTGTAAAGAAACTGTTGAAAATAAAGAGA
AGCAATGTGAAAAAAAAAAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_006115 unedited
NGTTTGGGAATTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGGG
AAACCGACTCCTGGGAGCAGGGAGGAACGCGCGCTCCAGAGACAACTTCGCGGTGTGGTG
AACTCTCTGAGGAAAAACACGTGCGTGGCAACAAGTGACTGAGACCTAGAAATCCAAGCG
TTGGAGGTCCTGAGGCCAGCCTAAGTCGCTTCAAAATGGAACGAAGGCGTTTGTGGGGTT
CCATTCAGAGCCGATACATCAGCATGAGTGTGTGGACAAGCCCACGGAGACTTGTGGAGC
TGGCAGGGCAGAGCCTGCTGAAGGATGAGGCCCTGGCCATTGCCGCCCTGGAGTTGCTGC
CCAGGGAGCTCTTCCCGCCACTCTTCATGGCAGCCTTTGACGGGAGACACAGCCAGACCC
TGAAGGCAATGGTGCAGGCCTGGCCCTTCACCTGCCTCCCTCTGGGAGTGCTGATGAAGG
GACAACATCTTCACCTGGAGACCTTCAAAGCTGTGCTTGATGGACTTGATGTGCTCCTTG
CCCAGGAGGTTCGCCCCAGGAGGTGGAAACTTCAAGTGCTGGATTTACGGAAGAACTCTC
ATCAGGACTTCTGGACTGTATGGTCTGGAAACAGGGCCAGTCTGTACTCATTTCCAGAGC
CAGAAGCAGCTCAGCCCATGACAAAGAAGCGAANAGTAGATGGTTTGAGCACAGAGGCAG
AGCAGCCCTTCATTCCAGTAGAGGTGCTCGTAGACCTGTTCCTCAAGAAGGTGCCTGTGA
TGAATTGTTCTCCTACCTCATTGAGAAAGTGAAGCGAAAGAAAAATGTACTACGCCTGTG
CTGTAAGAAGCTGAAAGATTTTGCATGCCCATGCNAGATATCAGAGATCCTGAAATGGTG
CAGCTGGACTCTATGGAGGATTNGGAGTGACTTGNACCTG
Restriction Sites Please inquire |
ACCN NM_006115
Insert Size 2076 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006115.3, NP_006106.1
RefSeq Size 2162 bp
RefSeq ORF 1530 bp
Locus ID 23532
UniProt ID P78395
Cytogenetics 22q11.22
Summary This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2014
Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a).
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Documents
Resources
"NM_006115" in other vectors (6)
SKU Description Size Price
RC214243 PRAME (Myc-DDK-tagged)-Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1 10 ug
$474.00
RC214243L1 Lenti ORF clone of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1, Myc-DDK-tagged 10 ug
$774.00
RC214243L2 Lenti ORF clone of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1, mGFP tagged 10 ug
$774.00
RC214243L3 Lenti ORF clone of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1, Myc-DDK-tagged 10 ug
$774.00
RC214243L4 Lenti ORF clone of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1, mGFP tagged 10 ug
$774.00
RG214243 PRAME (tGFP-tagged) - Human preferentially expressed antigen in melanoma (PRAME), transcript variant 1 10 ug
$489.00 $674.00

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