PGAM2 (NM_000290) Human Untagged Clone

SKU SC122557

PGAM2 (untagged)-Human phosphoglycerate mutase 2 (muscle) (PGAM2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PGAM2
• Synonyms: GSD10; PGAM-M; PGAMM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000290 edited; AATTCGGTACGAGGGTTGGGAAGCAGCCGTCCCCGTCCAGAGTCCTCTGTGGTCCCTGCT; GCCACCATGGCCACTCACCGCCTCGTGATGGTCCGGCACGGCGAGAGCACATGGAACCAG; GAGAACCGTTTCTGTGGCTGGTTCGATGCAGAGCTGAGTGAAAAGGGGACCGAGGAGGCC; AAGCGGGGAGCCAAGGCCATCAAGGATGCCAAGATGGAGTTTGACATCTGCTACACGTCA; GTGCTGAAGCGGGCCATCCGCACCCTCTGGGCCATCCTGGACGGCACGGACCAGATGTGG; CTGCCTGTGGTGCGCACTTGGCGCCTCAATGAGCGGCATTACGGGGGCCTCACAGGCCTC; AACAAGGCAGAAACGGCCGCCAAGCACGGGGAGGAGCAGGTGAAGATCTGGAGGCGCTCC; TTCGACATCCCGCCGCCCCCGATGGACGAGAAGCACCCCTACTACAACTCCATTAGCAAG; GAGCGTCGGTACGCAGGCCTGAAGCCCGGGGAACTCCCCACCTGCGAGAGCCTCAAGGAC; ACCATTGCCCGGGCCCTGCCCTTCTGGAACGAGGAGATTGTTCCCCAGATCAAGGCCGGC; AAGCGAGTGCTCATTGCAGCCCACGGGAACAGCCTGCGGGGCATTGTCAAGCACCTGGAA; GGGATGTCAGACCAGGCGATCATGGAGCTGAACCTGCCCACGGGGATCCCCATTGTGTAT; GAGCTGAACAAGGAGCTGAAGCCCACCAAGCCCATGCAGTTCCTGGGTGATGAGGAAACG; GTGCGGAAGGCCATGGAGGCTGTGGCTGCCCAGGGCAAGGCCAAGTGAGGGGTGGGCTTG; GGCAATAAAGGCACCTCCCCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000290 unedited; NAAGTCAAACATTGTAAACCACTCACAATAGGCGGCCCGCGAATCGGTACGAGGGTTGGG; AACCAGCCGTCCCCGTCCAGAGTCCTCTGTGGTCCCTGCTGCCACCATGGCCACTCACCG; CCTCGTGATGGTCCGGCACGGCGAGAGCACATGGAACCAGGAGAACCGTTTCTGTGGCTG; GTTCGATGCAGAGCTGAGTGAAAAGGGGACCGAGGAGGCCAAGCGGGGAGCCAAGGCCAT; CAAGGATGCCAAGATGGAGTTTGACATCTGCTACACGTCAGTGCTGAAGCGGGCCATCCG; CACCCTCTGGGCCATCCTGGACGGCACGGACCAGATGTGGCTGCCTGTGGTGCGCACTTG; GCGCCTCAATGAGCGGCATTACGGGGGCCTCACAGGCCTCAACAAGGCAGAAACGGCCGC; CAAGCACGGGGAGGAGCAGGTGAAGATCTGGAGGCGCTCCTTCGACATCCCGCCGCCCCC; GATGGACGAGAAGCACCCCTACTACAACTCCATTAGCAAGGAGCGTCGGTACGCAGGCCT; GAAGCCCGGGGAACTCCCCACCTGCGAGAGCCTCAAGGACACCATTGCCCGGGCCCTGCC; CTTCTGGAACGAGGAGATTGTTCCCCAGATCAAGGCCGGCAAGCGAGTGCTCATTGCAGC; CCACGGGAACAGCCTGCGGGGCATTGTCAAGCACCTGGAAGGGATGTCAGACCAGGCGAT; CATGGAGCTGAACCTGCCCACGGNGATCCCCATTGTGTATGAGCTGAACAGGAGCTGAAG; CCACCAAGCCATGCATTCCTGGGTGATGAGAAAACGTGCGGAANGCCATGNAGGCTGTGG; CTGCCCAGGCAGGCCAATGAGGGGTGGGCTGGGCATAAAGCACCTCCCCACAAAAAAAAA; AAAAAAAAAAAAACTCACTCTGA;
• Restriction Sites: Please inquire
• ACCN: NM_000290
• Insert Size: 896 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000290.2, NP_000281.2
• RefSeq Size: 845 bp
• RefSeq ORF: 762 bp
• Locus ID: 5224
• UniProt ID: P15259
• Cytogenetics: 7p13
• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways
• Summary: Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009]