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HSD11B2 (NM_000196) Human Untagged Clone

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SKU
SC122552
HSD11B2 (untagged)-Human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol HSD11B2
Synonyms AME; AME1; HSD2; HSD11K; SDR9C3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_000196 edited
CCCTCTCGCGCCCCAGGCCGGTGTACCCCCGCACTCCGCGCCCCGGCCTAGAAGCTCTCT
CTCCCCGCTCCCCGGCCCGGCCCCCGCCCCGCCCCGCCCCAGCCCGCTGGGCCGCCATGG
AGCGCTGGCCTTGGCCGTCGGGCGGCGCCTGGCTGCTCGTGGCTGCCCGCGCGCTGCTGC
AGCTGCTGCGCTCAGACCTGCGTCTGGGCCGCCCGCTGCTGGCGGCGCTGGCGCTGCTGG
CCGCGCTCGACTGGCTGTGCCAGCGCCTGCTGCCCCCGCCGGCCGCACTCGCCGTGCTGG
CCGCCGCCGGCTGGATCGCGTTGTCCCGCCTGGCGCGCCCGCAGCGCCTGCCGGTGGCCA
CTCGCGCGGTGCTCATCACCGGCTGTGACTCTGGTTTTGGCAAGGAGACGGCCAAGAAAC
TGGACTCCATGGGCTTCACGGTGCTGGCCACCGTATTGGAGTTGAACAGCCCCGGTGCCA
TCGAGCTGCGTACCTGCTGCTCCCCTCGCCTAAGGCTGCTGCAGATGGACCTGACCAAAC
CAGGAGACATTAGCCGCGTGCTAGAGTTCACCAAGGCCCACACCACCAGCACCGGCCTGT
GGGGCCTCGTCAACAACGCAGGCCACAATGAAGTAGTTGCTGATGCGGAGCTGTCTCCAG
TGGCCACTTTCCGTAGCTGCATGGAGGTGAATTTCTTTGGCGCGCTCGAGCTGACCAAGG
GCCTCCTGCCCCTGCTGCGCAGCTCAAGGGGCCGCATCGTGACTGTGGGGAGCCCAGCGG
GGGACATGCCATATCCGTGCTTGGGGGCCTATGGAACCTCCAAAGCGGCCGTGGCGCTAC
TCATGGACACATTCAGCTGTGAACTCCTTCCCTGGGGGGTCAAGGTCAGCATCATCCAGC
CTGGCTGCTTCAAGACAGAGTCAGTGAGAAACGTGGGTCAGTGGGAAAAGCGCAAGCAAT
TGCTGCTGGCCAACCTGCCTCAAGAGCTGCTGCAGGCCTACGGCAAGGACTACATCGAGC
ACTTGCATGGGCAGTTCCTGCACTCGCTACGCCTGGCCATGTCCGACCTCACCCCAGTTG
TAGATGCCATCACAGATGCGCTGCTGGCAGCTCGGCCCCGCCGCCGCTATTACCCCGGCC
AGGGCCTGGGGCTCATGTACTTCATCCACTACTACCTGCCTGAAGGCCTGCGGCGCCGCT
TCCTGCAGGCCTTCTTCATCAGTCACTGTCTGCCTCGAGCACTGCAGCCTGGCCAGCCTG
GCACTACCCCACCACAGGACGCAGCCCAGGGCCCAAACCTGAGCCCCGGCCCTTCCCCAG
CAGTGGCTCGGTGAGCCATGTGCACCTATGGCCCAGCCACTGCAGCACAGGAGGCTCCGT
GAGCCCTTGGTTCCTCCCCGAAAACCCCCAGCATTACGATCCCCCAAGTGTCCTGGACCC
TGGCCTAAAGAATCCCACCCCCACTTCATGCCCACTGCCGATGCCCAATCCAGGCCCGGT
GAGGCCAAGGTTTCCCAGTGAGCCTCTGCGCCTCTCCACTGTTTCATGAGCCCAAACACC
CTCCTGGCACAACGCTCTACCCTGCAGCTTGGAGAACTCCGCTGGATGGGGAGTCTCATG
CAAGACTTCACTGCAGCCTTTCACAGGACTCTGCAGATAGTGCCTCTGCAAACTAAGGAG
TGACTAGGTGGGTTGGGGACCCCCTCAGGATTGTTTCTCGGCACCAGTGCCTCAGTGCTG
CAATTGAGGGCTAAATCCCAAGTGTCTCTTGACTGGCTCAAGAATTAGGGCCCCAACTAC
ACACCCCCAAGCCACAGGGAAGCATGTACTGTACTTCCCAATTGCCACATTTTAAATAAA
GACAAATTTTTATTTCTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000196 unedited
GGGTTTGGATTTGTATACGACTCATATAGGCGGCCGCGACTTCCCGGGATCCCTCTCGCG
CCCCAGGCCGGTGTACCCCCGCACTCCGCGCCCCGGCCTAGAAGCTCTCTCTCCCCGCTC
CCCGGCCCGGCCCCCGCCCCGCCCCGCCCCAGCCCGCTGGGCCGCCATGGAGCGCTGGCC
TTGGCCGTCGGGCGGCGCCTGGCTGCTCGTGGCTGCCCGCGCGCTGCTGCAGCTGCTGCG
CTCAGACCTGCGTCTGGGCCGCCCGCTGCTGGCGGCGCTGGCGCTGCTGGCCGCGCTCGA
CTGGCTGTGCCAGCGCCTGCTGCCCCCGCCGGCCGCACTCGCCGTGCTGGCCGCCGCCGG
CTGGATCGCGTTGTCCCGCCTGGCGCGCCCGCAGCGCCTGCCGGTGGCCACTCGCGCGGT
GCTCATCACCGGCTGTGACTCTGGTTTTGGCAAGGAGACGGCCAAGAAACTGGACTCCAT
GGGCTTCACGGTGCTGGCCACCGTATTGGAGTTGAACAGCCCCGGTGCCATCGAGCTGCG
TACCTGCTGCTCCCCTCGCCTAAGGCTGCTGCAGATGGACCTGACCAAACCAGGAGACAT
TAGCCGCGTGCTAGAGTTCACCAAGGCCCACACCACCAGCACCGGCCTGTGGGGCCTCGT
CAACAACGCAGGCCACAATGAAGTAGTTGCTGATGCGGAGCTGTCTCCCATGGCCACTTT
CCGTAGCTGCATGGAGGTGAATTTCTTTGGCGCGCTCGAGCTGACCCAAGGCCTCCTGCC
TCTGCTGCGCAGCTAAAGGGCCGCATCGTGACTGTGGGGAGCCCACCGGGGGACATGCCA
TATCCGTGCTTGGGGGCCCAATGGAACCTCCAAAGGGCGCGTGGCCCTCCTCATGGACAC
ATTCGCCTGTGAACTCCT
Restriction Sites Please inquire |
ACCN NM_000196
Insert Size 1944 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000196.2, NP_000187.2
RefSeq Size 1944 bp
RefSeq ORF 1218 bp
Locus ID 3291
UniProt ID P80365
Cytogenetics 16q22.1
Protein Families Druggable Genome
Protein Pathways Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways
Summary There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. provided by RefSeq, Feb 2010
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Documents
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"NM_000196" in other vectors (6)
SKU Description Size Price
RC207796 HSD11B2 (Myc-DDK-tagged)-Human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2) 10 ug
$457.00
RC207796L1 Lenti ORF clone of Human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2), Myc-DDK-tagged 10 ug
$757.00
RC207796L2 Lenti ORF clone of Human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2), mGFP tagged 10 ug
$757.00
RC207796L3 Lenti ORF clone of Human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2), Myc-DDK-tagged 10 ug
$757.00
RC207796L4 Lenti ORF clone of Human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2), mGFP tagged 10 ug
$757.00
RG207796 HSD11B2 (tGFP-tagged) - Human hydroxysteroid (11-beta) dehydrogenase 2 (HSD11B2) 10 ug
$489.00 $657.00

file-alt Citations

27-hydroxylation of oncosterone by CYP27A1 switches its activity from pro-tumor to anti-tumor
Ayadi, Friedrichs, Soulès et al
J Lipid Res (2023) 64 (12), 100479 DOI: 10.1016/j.jlr.2023.100479
Cited by 17
AI Summary: OriGene’s pCMV6-XL5 HSD2 plasmid (SC122552) was used to express recombinant HSD2 in HEK-293T cells, enabling the measurement of OCDO synthase activity and facilitating the study of oncosterone metabolism in this paper.
Snippet: … About 5 × 106 HEK-293T cells were transfected with 5 μg of the plasmid pCMV6-XL5 HSD2 (SC122552, OriGene Technologies, Inc., Rockville, MD, USA) using the Neon Transfection System (Thermo Fisher Scientific, Waltham, MA, USA) with 2 pulses at 1300 V for 20 ms. 24 h after transfection, the media;h 5 μg of the plasmid pCMV6-XL5 HSD2 (SC122552, OriGene Technologies, Inc., Rockville, MD, USA) using the Neon Transfection System (Thermo Fisher Scientific, Waltham, MA, USA) with 2
27-hydroxylation of oncosterone by CYP27A1 switchs its activity from pro-tumor to anti-tumor
Ayadi, Friedrichs, Soulès et al
bioRxiv (2023)
Snippet: OCDO synthase activity was measured in HEK-293T cell lysates expressing recombinant HSD2. About 5 x 106 HEK-293T cells were transfected with 5 µg of the plasmid pCMV6-XL5 HSD2 (SC122552, OriGene Technologies, Inc., Rockville, MD, USA) using the Neon Transfection System (Thermo Fisher Scientific, Waltham, MA, USA) with 2 pulses at 1300 V for 20 ms
Chemical synthesis and biochemical properties of cholestane-5α,6β-diol-3-sulfonate: A non-hydrolysable analogue of cholestane-5α,6β-diol-3β-sulfate
de Médina, Ayadi, Soulès et al
J Steroid Biochem Mol Biol (2023) 234, 106396 DOI: 10.1016/j.jsbmb.2023.106396
Cited by 8
AI Summary: OriGene’s pCMV6-XL5 HSD2 plasmid (SC122552) was used to transfect HEK-293T cells for the expression of 11β-HSD2, enabling the study of CDSN’s inhibitory effects on this enzyme in the paper.
Snippet: HEK-293 T cells (5.106) were transfected with 5 µg of the plasmid pCMV6-XL5 HSD2 (SC122552, OriGene Technologies, Inc., Rockville, MD, USA) using the Neon Transfection System (Thermo Fisher Scientific, Waltham, MA, USA) with 2 pulses at 1300 V for 20 ms
Identification of a tumor-promoter cholesterol metabolite in human breast cancers acting through the glucocorticoid receptor
Voisin, de Medina, Mallinger et al
PNAS (2017) 114 (44), E9346-E9355 DOI: 10.1073/pnas.1707965114
AI Summary: OriGene contributed to this study by providing plasmids for 11HSD1 (sc109325), 11HSD2 (sc122552 or RG207796), H6PD (sc117481), DHCR7 (sc110871), and D8D7I (EBP), which were used to investigate the roles of these enzymes in cholesterol metabolism and breast cancer progression.
Snippet: The NEON Transfection system was purchased from Invitrogen, the BrdU cell proliferation Elisa kit was from Roche Diagnostics or Sigma, and the following plasmids were from OriGene: 11HSD1: sc109325;11HSD2: sc122552 or RG207796;H6PD: sc117481;DHCR7: sc110871;and D8D7I (EBP):
Showing 1-4 of 4 papers.
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