DIS3L (NM_133375) Human Untagged Clone

SKU SC120532

DIS3L (untagged)-Human DIS3 mitotic control homolog (S. cerevisiae)-like (DIS3L), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DIS3L
• Synonyms: DIS3L1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120532 sequence for NM_133375 edited (data generated by NextGen Sequencing); ATGCAGACAGCTTGTCAAGCTGTGCAGCATCAAAGAGGCAGGAGACAGTATAACAAACTG; CGAAACCTGCTGAAGGATGCGCGTCATGATTGCATTCTCTTTGCTAATGAATTCCAGCAA; TGCTGCTATCTGCCACGGGAAAGAGGAGAGTCCATGGAGAAGTGGCAGACCAGGAGCATA; TACAACGCAGCTGTTTGGTACTATCATCACTGCCAGGACAGGATGCCAATTGTTATGGTG; ACAGAAGATGAAGAGGCAATTCAGCAGTATGGAAGTGAAACAGAAGGAGTATTCGTGATT; ACTTTCAAGAATTACCTGGACAATTTCTGGCCTGATTTAAAAGCTGCCCACGAGCTTTGT; GATTCTATCCTTCAGTCTCGACGGGAGAGAGAGAATGAGAGTCAGGAGAGCCATGGGAAG; GAGTACCCAGAACATCTTCCCCTGGAAGTGTTAGAAGCTGGGATTAAATCTGGACGCTAT; ATCCAGGGAATTCTGAATGTCAACAAACACAGAGCCCAAATAGAAGCTTTTGTTCGACTT; CAAGGAGCCAGCAGTAAAGATTCAGATTTAGTCAGTGACATCCTAATCCACGGGATGAAG; GCTCGAAACCGCTCAATTCATGGAGATGTGGTAGTTGTGGAGCTGCTTCCTAAAAATGAA; TGGAAAGGAAGAACCGTAGCCCTGTGTGAGAATGACTGTGACGACAAGGCTTCGGGCGAG; TCCCCAAGTGAGCCCATGCCTACAGGTCGAGTGGTGGGCATACTTCAGAAGAACTGGCGG; GATTATGTGGTGACATTTCCGTCCAAAGAAGAGGTCCAATCTCAGGGCAAAAATGCTCAG; AAAATCCTGGTTACACCTTGGGATTACAGAATTCCCAAAATTCGAATTAGCACTCAGCAA; GCAGAAACCCTCCAGGACTTCAGGGTGGTCGTGCGCATCGATTCCTGGGAGTCAACATCT; GTGTATCCAAATGGACATTTTGTGCGTGTTTTAGGAAGAATCGGAGATCTGGAAGGGGAA; ATTGCAACCATCCTGGTGGAAAACAGTATTTCAGTTATTCCTTTCTCAGAAGCTCAGATG; TGTGAGATGCCAGTAAACACACCAGAAAGTCCCTGGAAGGTGAGTCCTGAAGAGGAACAA; AAACGTAAAGACTTGAGGAAAAGCCATCTCGTATTCAGCATTGACCCCAAAGGTTGTGAA; GATGTGGATGACACACTCTCAGTCAGAACCTTAAATAATGGCAACCTGGAACTTGGGGTC; CACATCGCAGATGTAACACACTTTGTGGCACCAAATTCTTACATTGATATTGAAGCTAGA; ACAAGGGCCACCACTTATTATCTAGCAGATCGTCGCTATGACATGCTGCCTTCCGTCCTC; AGTGCAGATTTGTGTTCCCTTCTGGGAGGCGTTGATAGGTATGCTGTAAGCATCATGTGG; GAACTGGATAAAGCCTCTTATGAAATTAAGAAAGTGTGGTATGGCAGAACCATTATTCGA; TCAGCATACAAACTGTTCTATGAAGCAGCCCAAGAACTACTGGATGGAAACTTAAGCGTT; GTTGATGATATTCCAGAATTCAAAGACTTGGGTGAGAAGAGCAGACAAGCCAAGCTGGAG; GAGTTGGTGTGGGCAATTGGAAAGCTGACCGACATAGCTCGCCATGTCAGAGCTAAACGA; GACGGATGTGGTGCCCTGGAACTGGAAGGGGTAGAGGTTTGCGTACAGCTAGATGACAAA; AAGAACATTCACGACCTCATCCCCAAGCAGCCCCTGGAAGTCCACGAGACAGTGGCTGAA; TGCATGATCCTGGCCAACCACTGGGTCGCCAAAAAGATCTGGGAGAGCTTCCCTCATCAG; GCCTTGCTGCGCCAGCACCCTCCTCCACACCAGGAGTTCTTTTCAGAACTCCGGGAATGT; GCTAAAGCCAAAGGCTTCTTCATAGATACACGGTCCAATAAAACACTGGCTGATTCTCTG; GATAATGCGAACGACCCCCACGATCCCATTGTGAACAGGCTACTGCGCTCCATGGCCACG; CAGGCCATGTCGAATGCTCTGTACTTCTCCACCGGATCCTGTGCGGAGGAGGAGTTCCAT; CATTACGGTCTTGCATTAGATAAATATACCCACTTTACTTCTCCAATAAGAAGATATTCA; GATATTGTAGTACACCGCTTGTTAATGGCAGCCATTTCAAAAGATAAGAAAATGGAAATT; AAGGGAAATCTGTTCAGCAACAAAGATCTTGAGGAATTATGCAGACATATCAACAACAGA; AACCAAGCAGCACAGCATTCTCAGAAGCAGTCTACTGAGCTCTTCCAGTGCATGTACTTC; AAAGACAAAGACCCTGCCACCGAGGAGCGTTGCATATCTGACGGAGTTATTTATTCAATT; AGAACAAATGGTGTGCTTCTATTTATACCAAGGTTTGGGATTAAAGGTGCTGCTTATCTA; AAAAATAAAGATGGTTTAGTCATCTCATGTGGCCCAGATAGCTGTTCTGAATGGAAACCA; GGATCCCTTCAACGATTTCAAAACAAAATTACCTCTACTACAACAGATGGGGAATCTGTT; ACGTTCCATTTGTTTGACCATGTAACCGTAAGAATATCCATACAGGCCTCACGTTGCCAT; TCTGATACAATCAGACTTGAAATAATTAGTAACAAACCATACAAGATACCAAATACAGAA; CTTATTCATCAGAGTTCCCCCTTGCTGAAGAGTGAGTTAGTGAAAGAAGTAACTAAATCT; GTGGAAGAAGCTCAGCTTGCCCAAGAAGTCAAAGTAAACATCATTCAGGAGGAATATCAA; GAATATCGCCAAACAAAGGGAAGGAGCCTATACACACTTCTAGAGGAGATACGGGACCTA; GCTCTCCTGGATGTTTCAAACAATTATGGAATATGA; ; Clone variation with respect to NM_133375.3; 1592 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133375 unedited; NGGGGTCCCCATTAGTATACGACTCATATAGGCGGCNCGCGATTCGGCACGAGGTTCTTC; ACCTGCCGTCCAGGTATCATCCTAGTCTTCCCTCCGTCTCTGGGATGGGAAACTCTTGTC; TAGTGATGTGACTCATTACGTGATCCCAGACTGGAAAGTTGTTCAAGATTATCTTGAGAT; CCTTGAGTTTCCTGAGTTGAAGGGAATTATTTTCATGCAGACAGCTTGTCAAGCTGTGCA; GCATCAAAGAGGCAGGAGACAGTATAACAAACTGCGAAACCTGCTGAAGGATGCGCGTCA; TGATTGCATTCTCTTTGCTAATGAATTCCAGCAATGCTGCTATCTGCCACGGGAAAGAGG; AGAGTCCATGGAGAAGTGGCAGACCAGGAGCATATACAACGCAGCTGTTTGGTACTATCA; TCACTGCCAGGACAGGATGCCAATTGTTATGGTGACAGAAGATGAAGAGGCAATTCAGCA; GTATGGAAGTGAAACAGAAGGAGTATTCGTGATTACTTTCAAGAATTACCTGGACAATTT; CTGGCCTGATTTAAAAGCTGCCCACGAGCTTTGTGATTCTATCCTTCAGTCTCGACGGGA; GAGAGAGAATGAGAGTCAGGAGAGCCATGGGAAGGAGTACCCAGAACATCTTCCCCTGNA; AGTGTTAGAAGCTGGGATTAAATCTGGACGCTATATCCAGGGAATTTCTGATGTCAACAA; ACACAGAGCCCAAATAGAAGCTTTTGTTCGACTTCAAAGAGCCAGCAGTAAAGATTCAGA; TTTAGTCAGTGACATCCTAATCCACGGGATGAAAGGCTCGAACCGCTCAATTCATGGAGA; TGTGGTANNTGTGGGAGCTGCTTTCCTAAAATGAATGGNAAGGGAGAACCGTACCCCTGT; GTGAGAATGACTGTGACA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_133375 unedited; CTACATCTAAGNAACCGCGGCCGCATTCTAGNGATCGATTTTTTTTTTTTTTTTTTGGAA; AGGAGTCTCGCTCTGTTGCCCAGGCTCGGAAGCAGTGGTGCGATCTCGGCTCACTGCAAC; CTCCTCTTCCTGGGTTCAGGCAATTCTCCTGCTTCAGCCTCCCAAGTAGCTGGGACTACA; GGCGCATGCCACCACGCCGGGCTAATTTTTGTATTTTTAGTAGAGACTGGGTTTCACCGT; GTTAGCCAGGATGGTCTCAATCTCCTGACCTCGTGATCCGCCCGCCTCAGCCTCCCAAAG; TGCTGGGGTTACAGGCGTGAGCCACCGTGCCCGGCTGAGAACATTTTACATATATGCGAA; ATAGCTACCCAGTCCTGATCGACTAGAGCACTGAGTGACACACATTAAATGTTAAGTTTG; AAAAGACTGTAAAACATTCACATATGACAGCTCTTAGTGAAGTAAGAGCCTCTCATATTC; CATAATTGTTTGAAACATCCAGGAGAGCTAGGTCCCGTATCTCCTCTAGAAGTGTGTATA; GGCTCCTTCCCTTTGTTTGGCGATATTCTTGATATTCCTCCTGAATGATGTTTACTTTGA; CTTCTTGGGCAAGCTGAGCTTCTTCCACAGATTTAGTTACTTCTTTCACTAACTCACTCT; TCAGCAAGGGGGAACTCTGATGAATAAGTTCTGTATTTGGTATCTTGTATGGTTTGTTAC; TAATTATTTCAAGTCTGATTGTATCAGAATGGCAACGTGGAGCCTGTATGGATATTCTTA; CGGTTACATGGTCAAACCNATGGAACGTAACAGATTCCCCATCTGTTGTAGTAGAGGTAA; TTTTGTTTTGAAATCTTTGAAGGATCCTGGTTCCATTCAAACAGCTATCTGGGCCCATGA; AATT;
• Restriction Sites: NotI-NotI
• ACCN: NM_133375
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_133375.2, NP_588616.1
• RefSeq Size: 3685 bp
• RefSeq ORF: 2916 bp
• Locus ID: 115752
• UniProt ID: Q8TF46
• Cytogenetics: 15q22.31
• Domains: RNB
• Summary: The cytoplasmic RNA exosome complex degrades unstable mRNAs and is involved in the regular turnover of other mRNAs. The protein encoded by this gene contains 3'-5' exoribonuclease activity and is a catalytic component of this complex. [provided by RefSeq, May 2016]; Transcript Variant: This variant (2) represents an alternate first exon and the transcript has a smaller open reading frame compared to variant 1. The resulting protein isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.