CD97 (ADGRE5) (NM_078481) Human Untagged Clone

SKU SC120259

CD97 (untagged)-Human CD97 molecule (CD97), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CD97
• Synonyms: CD97; TM7LN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_078481, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGGAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCA; GGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAATCCAGG; GTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAGACTTGTGACGACATCAACGAGTGTGCAACA; CCGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCA; GCCCGGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGT; GGACGAATGTCAGCAGAACCCAAGGCTCTGTAAAAGCTACGGCACCTGCGTCAACACCCTTGGCAGCTAT; ACCTGCCAGTGCCTGCCTGGCTTCAAGTTCATACCTGAGGATCCGAAGGTCTGCACAGATGTGAATGAAT; GCACCTCCGGACAAAACCCGTGCCACAGCTCCACCCACTGCCTCAACAACGTGGGCAGCTATCAGTGCCG; CTGCCGCCCGGGCTGGCAACCGATTCCGGGGTCCCCCAATGGCCCAAACAATACCGTCTGTGAAGATGTG; GACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACA; GCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTGTCTGTGA; AGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCTTCGAC; AAAGTCCAGGACCTGGGCAGAGACTCCAAGACAAGCTCAGCCGAGGTCACCATCCAGAATGTCATCAAAT; TGGTGGATGAACTGATGGAAGCTCCTGGAGACGTAGAGGCCCTGGCGCCACCTGTCCGGCACCTCATAGC; CACCCAGCTGCTCTCAAACCTTGAAGATATCATGAGGATCCTGGCCAAGAGCCTGCCTAAAGGCCCCTTC; ACCTACATTTCCCCTTCGAACACAGAGCTGACCCTGATGATCCAGGAGCGGGGGGACAAGAACGTCACTA; TGGGTCAGAGCAGCGCACGCATGAAGCTGAATTGGGCTGTGGCAGCTGGAGCCGAGGATCCAGGCCCCGC; CGTGGCGGGCATCCTCTCCATCCAGAACATGACGACATTGCTGGCCAATGCCTCCTTGAACCTGCATTCC; AAGAAGCAAGCCGAACTGGAGGAGATATATGAAAGCAGCATCCGTGGTGTCCAACTCAGACGCCTCTCTG; CCGTCAACTCCATCTTTCTGAGCCACAACAACACCAAGGAACTCAACTCCCCCATCCTTTTCGCCTTCTC; CCACCTTGAGTCCTCCGATGGGGAGGCGGGAAGAGACCCTCCTGCCAAGGACGTGATGCCTGGGCCACGG; CAGGAGCTGCTCTGTGCCTTCTGGAAGAGTGACAGCGACAGGGGAGGGCACTGGGCCACCGAGGGCTGCC; AGGTGCTGGGCAGCAAGAACGGCAGCACCACCTGCCAATGCAGCCACCTGAGCAGCTTTGCGATCCTTAT; GGCTCATTATGACGTGGAGGACTGGAAGCTGACCCTGATCACCAGGGTGGGACTGGCGCTGTCACTCTTC; TGCCTGCTGCTGTGCATCCTCACTTTCCTGCTGGTGCGGCCCATCCAGGGCTCGCGCACCACCATACACC; TGCACCTCTGCATCTGCCTCTTCGTGGGCTCCACCATCTTCCTGGCCGGCATCGAGAACGAAGGCGGCCA; GGTGGGGCTGCGCTGCCGCCTGGTGGCCGGGCTGCTGCACTACTGTTTCCTGGCCGCCTTCTGCTGGATG; AGCCTCGAAGGCCTGGAGCTCTACTTTCTTGTGGTGCGCGTGTTCCAAGGCCAGGGCCTGAGTACGCGCT; GGCTCTGCCTGATCGGCTATGGCGTGCCCCTGCTCATCGTGGGCGTCTCGGCTGCCATCTACAGCAAGGG; CTACGGCCGCCCCAGATACTGCTGGTTGGACTTTGAGCAGGGCTTCCTCTGGAGCTTCTTGGGACCTGTG; ACCTTCATCATTTTGTGCAATGCTGTCATTTTCGTGACTACCGTCTGGAAGCTCACTCAGAAGTTTTCTG; AAATCAATCCAGACATGAAGAAATTAAAGAAGGCGAGGGCGCTGACCATCACGGCCATCGCGCAGCTCTT; CCTGTTGGGCTGCACCTGGGTCTTTGGCCTGTTCATCTTCGACGATCGGAGCTTGGTGCTGACCTATGTG; TTTACCATCCTCAACTGCCTGCAGGGCGCCTTCCTCTACCTGCTGCACTGCCTGCTCAACAAGAAGGTTC; GGGAAGAATACCGGAAGTGGGCCTGCCTAGTTGCTGGGGGGAGCAAGTACTCAGAATTCACCTCCACCAC; GTCTGGCACTGGCCACAATCAGACCCGGGCCCTCAGGGCATCAGAGTCCGGCATATGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_078481 unedited; GGGGCCCGGGGGAAGNNGCAAATTCCCNNNGGNGTTCGGAATTGAAACCCATTTATATAG; GCGGACGCGCAATTCGCACCANAACGCCAGTTCCGGGGAGGGCCCTGGGCCAGCGGCTGT; CCGCCCCCCCTCCTTCATAAAGTCCTGGCCTCGGGACAGCCTGCACAGCTGCCTAGCCTG; TGGAGACGGGACAGCCCTGTCCCACTCACTCTTTCCCCTGCCGCTCCTGCCGGCAGCTCC; AACCATGGTAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGA; AACCCAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGC; CACCGCCTGTCGCTGCAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCGAC; GGAGACTTGTGACGACATCAACGAGTGTGCAACACCGTCGAAAGTGTCATGCGGAAAATT; CTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCGGGATATGAGCC; TGTNTCTGGNGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGTGGACGA; ATGTCAGCAGAACCCAAGGCTCTGNTAAAGCTACGGCACCTGCGTCAACACCCTTGGCAG; CTATACCTGCCAGTGCCTGCCTGGCTTAAAGTCATACCTGAGGATCCGAAGGTCTGCACA; GATGTGAATGAATGCACCTNCGGGACANAACCCCGTGCACAGNNTCCACCCACTGCCTCA; ACAACGTGGGGCAGCTATCANTGCCGCTGCCNGCCGGCCTGGCANCCCGATCCCGGGGTC; CCCACATGGCCCCAACAATACCCTTTGTGAAGAATGTGGACAATGCANNTCCGGGCAGCA; TAAGTGTGACAGGTCCACCGTTTGGTTAAAACCCGGGGTTATACAGCTGCCCGTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_078481 unedited; ACAAACTATGGTCGTTATCCCTCTTCTAGATTTGTTTTTTTTTTTTTTTTTTTTTTTTTT; TGGATGCATGTGTTTAATTTTAAGTGTCAACACTGGAAAAATTTTAACAGATAAAAACAC; ATTACACAACGTCTACAACCTTCACAACAGTGAGAACCTCCTGCCCGGCCAGGCAGGGCA; GGCCTCTGTACCGTGAGCCTCAAGCAGTGGCCCCAAGGGGCCTCGCCATGGGGCCTCTGA; CATCAGTCTTAGCTTAAGAGGGAAGCTGAGCCCCAGCGCCTAGTCTCTTCAGTTCTGCAG; CAAAGACATGAGAGGAAAAGTCCAGGATGGGACAAGCGCCCTTAGTCTGTGCCCGGAAGN; TTNAACCTAGGGGGCCCCACCAGAGGTTGCCCCAGGGGGGCAAAACATTGGCTTGGCATT; TGCCAAGCCCCCTGGGGCCCCAACCCCCCTTGTTCCCCCACCCCTGGGGGTCCACCAAAG; GTGGAAACCANAAGAAGGCAGGCCCAGCAAGGCAGGAACCCGGTGGGGTTTCCGAATTTC; CACCCCCACTTGGGGGTGGTCCCCGGGAACTTTTTGGGTGGCCCACGAACTATTAGGTTG; GGCCATTCCCCTTCCCTTGGGGGGGCCCCCCGGGGTTTTCAAAGGAAAAAGGAAAGGTTG; GGGAAGGGGGTTTTTTAAAATTGGGGGGGGGGGGACCCCAAAAAAGGGGGNNGGGNNNTT; NNNNNNNNNNNNCNCTTTTTTTTTGTTTCCNNNNAAAAAAAAAANNNCCTNNTTTTGTGG; GGGCTTCTCCCNNNNNNTNNNNTCCCCCTTNGGNGGTNNCCCCCCCCCCCCCCNNNNNTT; TCCCTTTTTGGGGTGTTGTTTGTCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_078481
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_078481.2, NP_510966.1
• RefSeq Size: 3247 bp
• RefSeq ORF: 2508 bp
• Locus ID: 976
• UniProt ID: P48960
• Cytogenetics: 19p13.12
• Domains: 7tm_2, EGF, EGF_CA, GPS
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, GPCR, Secreted Protein, Transmembrane
• Summary: This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as CD97(EGF 1,2,3,4,5)).