Cyclin E2 (CCNE2) (NM_057749) Human Untagged Clone

SKU SC120236

CCNE2 (untagged)-Human cyclin E2 (CCNE2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cyclin E2
• Synonyms: CYCE2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120236 sequence for NM_057749 edited (data generated by NextGen Sequencing); ATGTCAAGACGAAGTAGCCGTTTACAAGCTAAGCAGCAGCCCCAGCCCAGCCAGACGGAA; TCCCCCCAAGAAGCCCAGATAATCCAGGCCAAGAAGAGGAAAACTACCCAGGATGTCAAA; AAAAGAAGAGAGGAGGTCACCAAGAAACATCAGTATGAAATTAGGAATTGTTGGCCACCT; GTATTATCTGGGGGGATCAGTCCTTGCATTATCATTGAAACACCTCACAAAGAAATAGGA; ACAAGTGATTTCTCCAGATTTACAAATTACAGATTTAAAAATCTTTTTATTAATCCTTCA; CCTTTGCCTGATTTAAGCTGGGGATGTTCAAAAGAAGTCTGGCTAAACATGTTAAAAAAG; GAGAGCAGATATGTTCATGACAAACATTTTGAAGTTCTGCATTCTGACTTGGAACCACAG; ATGAGGTCCATACTTCTAGACTGGCTTTTAGAGGTATGTGAAGTATACACACTTCATAGG; GAAACATTTTATCTTGCACAAGACTTTTTTGATAGATTTATGTTGACACAAAAGGATATA; AATAAAAATATGCTTCAACTCATTGGAATTACCTCATTATTCATTGCTTCCAAACTTGAG; GAAATCTATGCTCCTAAACTCCAAGAGTTTGCTTACGTCACTGATGGTGCTTGCAGTGAA; GAGGATATCTTAAGGATGGAACTCATTATATTAAAGGCTTTAAAATGGGAACTTTGTCCT; GTAACAATCATCTCCTGGCTAAATCTCTTTCTCCAAGTTGATGCTCTTAAAGATGCTCCT; AAAGTTCTTCTACCTCAGTATTCTCAGGAAACATTCATTCAAATAGCTCAGCTTTTAGAT; CTGTGTATTCTAGCCATTGATTCATTAGAGTTCCAGTACAGAATACTGACTGCTGCTGCC; TTGTGCCATTTTACCTCCATTGAAGTGGTTAAGAAAGCCTCAGGTTTGGAGTGGGACAGT; ATTTCAGAATGTGTAGATTGGATGGTACCTTTTGTCAATGTAGTAAAAAGTACTAGTCCA; GTGAAGCTGAAGACTTTTAAGAAGATTCCTATGGAAGACAGACATAATATCCAGACACAT; ACAAACTATTTGGCTATGCTGGAGGAAGTAAATTACATAAACACCTTCAGAAAAGGGGGA; CAGTTGTCACCAGTGTGCAATGGAGGCATTATGACACCACCGAAGAGCACTGAAAAACCA; CCAGGAAAACACTAA; ; Clone variation with respect to NM_057749.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_057749 unedited; TGTCAGCATTTGTATACGACTCATATAGGCGGCCTGCGAATCGGCACGAGGGAGGTTTTA; TACACCTGAAAGAGAGATGTCAAGACGAAGTAGCCGTTTACAAGCTAAGCAGCAGCCCCA; GCCCAGCCAGACGGAATCCCCCCAAGAAGCCCAGATAATCCAGGCCAAGAAGAGGAAAAC; TACCCAGGATGTCAAAAAAAGAAGAGAGGAGGTCACCAAGAAACATCAGTATGAAATTAG; GAATTGTTGGCCACCTGTATTATCTGGGGGGATCAGTCCTTGCATTATCATTGAAACACC; TCACAAAGAAATAGGAACAAGTGATTTCTCCAGATTTACAAATTACAGATTTAAAAATCT; TTTTATTAATCCTTCACCTTTGCCTGATTTAAGCTGGGGATGTTCAAAAGAAGTCTGGCT; AAACATGTTAAAAAAGGAGAGCAGATATGTTCATGACAAACATTTTGAAGTTCTGCATTC; TGACTTGGAACCACAGATGAGGTCCATACTTCTAGACTGGCTTTTAGAGGTATGTGAAGT; ATACACACTTCATAGGGAAACATTTTATCTTGCACAAGACTTTTTTGATAGATTTATGTT; GACACAAAAGGATATAAATAAAAATATGCTTCAACTCATTGGAATTACCTCATTATTCAT; TGCTTCCAAACTTGAGGAAATCTATGCTCCTAAACTCCAAGAGTTTGCTTACGTCACTGA; TGGTGCTTGCAGTGAAGAGGATATCTTAAGGATGGAACTCATTATATTAAAGGCTTTACA; ATGGGAACTNTGTCCTGNTACAATCATCTCCTGGCTAAATCTCTTTCNTCCAGNTGATGC; TCTTAAAGAGCTCCTACAGTCTTCTACCTCAGTATTCTCAGGAACATTCATTCAATAGCT; CAGCTTTTA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_057749 unedited; NNTTTTGCTTTGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAAA; TTTTTTTAACTTTTATTTTTTAAACAATGGGGCTAAAAATAAACAGTATTAAAAGGTTAA; GTTTATATAATACATATGTACACAATTAGTGGTGTTTTCTTTTCAGACAAAATACTGAAA; CAAATATTAGTTTAAAAACAAACTATACAGAAGACTTCATACCGTAACAATAAATGTATA; GTTTCTTCAAAGGGAGAAGAGATTCACATATCTGATAACAAAATAAACTAGCAATCTAGT; TTTCTAATCTACTTTATGAGGCTGGATTTTTTTTTTAGAAAAGCTAATTTAAAATATTTA; GAAATAGCTAGCCTATGTACAGCAAGTTTTCATGTCTTTTTTTAATAAATAGATTTCTAG; GAGTCAGTATATATTTAATACTCTTCTTCCTTAAGAAAATAGAAGTTTAGGTCAAGTGTT; AAGCTTTATCACTTTGACACTGTCCTTATCTCACAATGGAGGAATTTAGAAAGGACCTTA; ACAGTTTCACAAACATAAATAAAGCCTTAGTCACACTAAATTAAAAAAAAAATTCCTTAG; GGATATCTTAGAGTAGTAAAGTGACTTCCTCATATAAATAGTTTGAAAGGGTACTTAAGT; TTTTCACCCAAATTGTGATATACAAAAAGGTTATTACCAAGCAACCTACATGTCAAGAAA; GCCCCAGTTAGGAAGGAGCCACAGCATTTATCTTGTTTATAATTTCTTTGGGACTCCCAC; TGTTTAGAGCACAGGTTGAACACCATGTTCATCTAAGCCTTATTAGTTAAAAAATGTGTT; ATGGGCAAGGCAATAAACTAGTTTAAAAACATTAAATTCACCATTTGTAGAATTCAAGTT; TATAATAGCTTGCTTAGCAGCATAGATAAAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_057749
• Insert Size: 2960 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_057749.1, NP_477097.1
• RefSeq Size: 2748 bp
• RefSeq ORF: 1215 bp
• Locus ID: 9134
• UniProt ID: O96020
• Cytogenetics: 8q22.1
• Domains: cyclin, CYCLIN, cyclin_C
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer
• Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008]