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XPB (ERCC3) (NM_000122) Human Untagged Clone

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SKU
SC120091
ERCC3 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3)
  $717.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol XPB
Synonyms BTF2; GTF2H; RAD25; Ssl2; TFIIH; TTD2; XPB
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_000122, the custom clone sequence may differ by one or more nucleotides


ATGGGCAAAAGAGACCGAGCGGACCGCGACAAGAAGAAATCCAGGAAGCGGCACTATGAGGATGAAGAGG
ATGATGAAGAGGACGCCCCGGGGAACGACCCTCAGGAAGCGGTTCCCTCGGCGGCGGGGAAGCAGGTGGA
TGAGTCAGGCACCAAAGTGGATGAATATGGAGCCAAGGACTACAGGCTGCAAATGCCGCTGAAGGACGAC
CACACCTCCAGGCCCCTCTGGGTGGCTCCCGATGGCCATATCTTCTTGGAAGCCTTCTCTCCAGTTTACA
AATATGCCCAAGACTTCTTGGTGGCTATTGCAGAGCCAGTGTGCCGACCAACCCATGTGCATGAGTACAA
ACTAACTGCCTACTCCTTGTATGCAGCTGTCAGCGTTGGGCTGCAAACCAGTGACATCACCGAGTACCTC
AGGAAGCTCAGCAAGACTGGAGTCCCTGATGGAATTATGCAGTTTATTAAGTTGTGTACTGTCAGCTATG
GAAAAGTCAAGCTGGTCTTGAAGCACAACAGATACTTCGTTGAAAGTTGCCACCCTGATGTAATCCAGCA
TCTTCTCCAGGACCCCGTGATCCGAGAATGCCGCTTAAGAAACTCTGAAGGGGAGGCCACTGAGCTCATC
ACAGAGACTTTCACAAGCAAATCTGCCATTTCTAAGACTGCTGAAAGCAGTGGTGGGCCCTCCACTTCCC
GAGTGACAGATCCACAGGGTAAATCTGACATCCCCATGGACCTGTTTGACTTCTATGAGCAAATGGACAA
GGATGAAGAAGAAGAAGAAGAGACACAGACAGTGTCTTTTGAAGTCAAGCAGGAAATGATTGAGGAACTC
CAGAAACGTTGCATCCACCTGGAGTACCCTCTGTTGGCAGAATATGACTTCCGGAATGATTCTGTCAACC
CTGATATCAACATTGACCTAAAGCCCACAGCTGTCCTCAGACCCTATCAGGAGAAGAGCTTGCGAAAGAT
GTTTGGAAACGGGCGTGCACGTTCGGGGGTCATTGTTCTTCCCTGCGGTGCTGGAAAGTCCCTGGTTGGT
GTGACTGCTGCATGCACTGTCAGAAAACGCTGTCTGGTGCTGGGCAACTCAGCTGTTTCTGTGGAGCAGT
GGAAAGCCCAGTTCAAGATGTGGTCCACCATTGACGACAGCCAGATCTGCCGGTTCACCTCCGATGCCAA
GGACAAGCCCATCGGCTGCTCCGTTGCCATTAGCACCTACTCCATGCTGGGCCACACCACCAAAAGGTCC
TGGGAGGCCGAGCGAGTCATGGAGTGGCTCAAGACCCAGGAGTGGGGCCTCATGATCCTGGATGAAGTGC
ACACCATACCAGCCAAGATGTTCCGAAGGGTGCTCACCATCGTGCAGGCCCACTGTAAGCTGGGTTTGAC
TGCGACCCTCGTCCGCGAAGATGACAAAATTGTGGATTTAAATTTTCTGATTGGGCCTAAGCTCTACGAA
GCCAACTGGATGGAGCTGCAGAATAATGGCTACATCGCCAAAGTCCAGTGTGCTGAGGTCTGGTGCCCTA
TGTCTCCTGAATTTTACCGGGAATATGTGGCAATCAAAACCAAGAAACGAATCTTGCTGTACACCATGAA
CCCCAACAAATTTAGAGCTTGCCAGTTTCTGATCAAGTTTCATGAAAGGAGGAATGACAAGATTATTGTC
TTTGCTGACAATGTGTTTGCCCTAAAGGAATATGCCATTCGACTGAACAAACCCTATATCTACGGACCTA
CGTCTCAGGGGGAAAGGATGCAAATTCTCCAGAATTTCAAGCACAACCCCAAAATTAACACCATCTTCAT
ATCCAAGGTAGGTGACACTTCGTTTGATCTGCCGGAAGCAAATGTCCTCATTCAGATCTCATCCCATGGT
GGCTCCAGGCGTCAGGAAGCCCAAAGGCTAGGGCGGGTGCTTCGAGCTAAAAAAGGGATGGTTGCAGAAG
AGTACAATGCCTTTTTCTACTCACTGGTATCCCAGGACACACAGGAAATGGCTTACTCAACCAAGCGGCA
GAGATTCTTGGTAGATCAAGGTTATAGCTTCAAGGTGATCACGAAACTCGCTGGCATGGAGGAGGAAGAC
TTGGCGTTTTCGACAAAAGAAGAGCAACAGCAGCTCTTACAGAAAGTCCTGGCAGCCACTGACCTGGATG
CCGAGGAGGAGGTGGTGGCTGGGGAATTTGGCTCCAGATCCAGCCAGGCATCTCGGCGCTTTGGCACCAT
GAGTTCTATGTCTGGGGCCGACGACACTGTGTACATGGAGTACCACTCATCGCGGAGCAAGGCGCCCAGC
AAACATGTACACCCGCTCTTCAAGCGCTTTAGGAAATGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_000122 unedited
ATATCCAGATTTGTATACGACTCATATGGGCGGCCGCGCAATTCGCACGAGGGCGGGCCT
GTGGGAGCGGGGTCATCTTCTCTCTGCTGCTGTAGCTGCCATGGGCAAAAGAGACCGAGC
GGACCTGCGACAAGTAAGAAATCCAGGAAGCGGCACTATGAGGATGAAGAGGATGATGAA
GAGGACGCCCCGGGGAACGACCCTCAGGAAGCGGTTCCCTCGGCGGCGGGGAAGCAGGTG
GATGAGTCAGGCACCAAAGTGGATGAATATGGAGCCAAGGACTACAGGCTGCAAATGCCG
CTGAAGGACGACCACACCTCCAGGCCCCTCTGGGTGGCTCCCGATGGCCATATCTTCTTG
GAAGCCTTCTCTCCAGTTTACAAATATGCCCAAGACTTCTTGGTGGCTATTGCAGAGCCA
GTGTGCCGACCAACCCATGTGCATGAGTACAAACTAACTGCCTACTCCTTGTATGCAGCT
GTCAGCGTTGGGCTGCAAACCAGTGACATCACCGAGTACCTCAGGAAGCTCAGCAAGACT
GGAGTCCCTGATGGAATTATGCAGTTTATTAAGTTGTGTACTGTCAGCTATGGAAAAGTC
AAGCTGGTCTTGAAGCACAACAGGTAAGAGATTCCATGACAGGCCTGTCCCCAGGCACTG
TCACTTTCTGCGCACCAGCAGGAATAAAGGGATATGGGCAGGACCTGATGTTTATGCTGT
CTTTTCTGTGAGCCCAGATAAGAGCAGAGAAACAGAGATGTGCCCCGACTGGAACTCATC
CCTGCCCACTGTATTCCTCTAGCATGGAGCTGGCTCAGCCCCTGCCTACTTGCCTCAGGT
CCCACCCTCTATGTTAAATCATGCCGCAGCTCATGCTCAGANTTGGCGAAGGGTGTGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000122 unedited
NTTTTACTATGGACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTCGGGGTCAAGGGGCCATTCTGTTTATTCAAAACGGGAA
CATAAATACCCCTTAAATATTAACATTTTTTATATACAGAAATGACCCCACTCCCCAAAA
AGTTTGAAAAAATATTGGCTCCTCCTCCTTTATAAAACCCTAAATGACCTATGAAGGCAC
AGCCAAGCCCTTGATGCATTTTCCTAAGCACAATTTGGCCAACGCTGGAGGGAAGGTCAA
AGAGGGGGAAGGAAAATGTTATGCTGAAAATCCCTTTCCAACAAGGGGGCCAAGCGCCGG
TCTTGAACAAAGTACCCTGCCTAAGCATCATTTCCTAAAGCGCTTGAAAAGCGGGTGTAC
ATGTTTGCTGGGCGCCTTGCTCCGCGATGAGGGGTACTCCATGTACACAGGGTCGTCGGC
CCCAAACATAAAACTCATGGGGCCAAAGCGCCGAAATGCCTGGCTGGATCTGGAGCCAAA
TTCCCCAGCCACCACCTCCTCCTGGGAATCCAGGTCAGGGGCTGCCAGGACTTTCTGTAA
AAGCTGCTGTTGCTCTTCTTTTGTCGAAAACGCCAAGTTTTCCTCCTCCATGCCAGCGAG
TTTCGTGATCACCTTGAAGCTATAACCTTGATCTACCAAGAATCTCTGCCGCTTGGTTGA
GTAAGCCATTTCCTGTGTGTCCTGGGATACCAGGGAGTAGAAAAAGGCATTGTACTCTTC
TGCAACCATCCCTTTTTTAGCTCGAAGCACCCGCCCTAACCTTTGGGCTTCCTGACGCCT
GGAGCCCCATGGGATGAGATCTGAATGAGACATTTGCTCCGGCAATCAAACGAGTGCACC
TACCTTGGATTGAAAATGGNGTATTTTGGGTTG
Restriction Sites NotI-NotI |
ACCN NM_000122
Insert Size 3260 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000122.1, NP_000113.1
RefSeq Size 2751 bp
RefSeq ORF 2349 bp
Locus ID 2071
UniProt ID P19447
Cytogenetics 2q14.3
Domains DEAD, helicase_C
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Nucleotide excision repair
Summary This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2014
Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (a).
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Documents
Resources
"NM_000122" in other vectors (7)
SKU Description Size Price
RC202425 ERCC3 (Myc-DDK-tagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3) 10 ug
$716.00
RC202425L1 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3), Myc-DDK-tagged 10 ug
$1,016.00
RC202425L2 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3), mGFP tagged 10 ug
$1,016.00
RC202425L3 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3), Myc-DDK-tagged 10 ug
$1,016.00
RC202425L4 Lenti ORF clone of Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3), mGFP tagged 10 ug
$1,016.00
RG202425 ERCC3 (tGFP-tagged) - Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3) 10 ug
$789.00 $916.00
SC320745 ERCC3 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) (ERCC3) 10 ug
$717.00

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