Integrin alpha 6 (ITGA6) (NM_000210) Human Untagged Clone

SKU SC120055

ITGA6 (untagged)-Human integrin, alpha 6 (ITGA6), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Integrin alpha 6
• Synonyms: CD49f; ITGA6B; VLA-6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000210 edited; GGGCGGCCCGCGAATTCGGCACCAGGGCCGCTGCAGGTCCCCGCTCCCCTCCCCGTGCGT; CCGCCCATGGCCGCCGCCGGGCAGCTGTGCTTGCTCTACCTGTCGGCGGGGCTCCTGTCC; CGGCTCGGCGCAGCCTTCAACTTGGACACTCGGGAGGACAACGTGATCCGGAAATATGGA; GACCCCGGGAGCCTCTTCGGCTTCTCGCTGGCCATGCACTGGCAACTGCAGCCCGAGGAC; AAGCGGCTGTTGCTCGTGGGGGCCCCGCGGGCAGAAGCGCTTCCACTGCAGAGAGCCAAC; AGAACGGGAGGGCTGTACAGCTGCGACATCACCGCCCGGGGGCCATGCACGCGGATCGAG; TTTGATAACGATGCTGACCCCACGTCAGAAAGCAAGGAAGATCAGTGGATGGGGGTCACC; GTCCAGAGCCAAGGTCCAGGGGGCAAGGTCGTGACATGTGCTCACCGATATGAAAAAAGG; CAGCATGTTAATACGAAGCAGGAATCCCGAGACATCTTTGGGCGGTGTTATGTCCTGAGT; CAGAATCTCAGGATTGAAGACGATATGGATGGGGGAGATTGGAGCTTTTGTGATGGGCGA; TTGAGAGGCCATGAGAAATTTGGCTCTTGCCAGCAAGGTGTAGCAGCTACTTTTACTAAA; GACTTTCATTACATTGTATTTGGAGCCCCGGGTACTTATAACTGGAAAGGGATTGTTCGT; GTAGAGCAAAAGAATAACACTTTTTTTGACATGAACATCTTTGAAGATGGGCCTTATGAA; GTTGGTGGAGAGACTGAGCATGATGAAAGTCTCGTTCCTGTTCCTGCTAACAGTTACTTA; GGTTTTTCTTTGGACTCAGGGAAAGGTATTGTTTCTAAAGATGAGATCACTTTTGTATCT; GGTGCTCCCAGAGCCAATCACAGTGGAGCCGTGGTTTTGCTGAAGAGAGACATGAAGTCT; GCACATCTCCTCCCTGAGCACATATTCGATGGAGAAGGTCTGGCCTCTTCATTTGGCTAT; GATGTGGCGGTGGTGGACCTCAACAAGGATGGGTGGCAAGATATAGTTATTGGAGCCCCA; CAGTATTTTGATAGAGATGGAGAAGTTGGAGGTGCAGTGTATGTCTACATGAACCAGCAA; GGCAGATGGAATAATGTGAAGCCAATTCGTCTTAATGGAACCAAAGATTCTATGTTTGGC; ATTGCAGTAAAAAATATTGGAGATATTAATCAAGATGGCTACCCAGATATTGCAGTTGGA; GCTCCGTATGATGACTTGGGAAAGGTTTTTATCTATCATGGATCTGCAAATGGAATAAAT; ACCAAACCAACACAGGTTCTCAAGGGTATATCACCTTATTTTGGATATTCAATTGCTGGA; AACATGGACCTTGATCGAAATTCCTACCCTGATGTTGCTGTTGGTTCCCTCTCAGATTCA; GTAACTATTTTCAGATCCCGGCCTGTGATTAATATTCAGAAAACCATCACAGTAACTCCT; AACAGAATTGACCTCCGCCAGAAAACAGCGTGTGGGGCGCCTAGTGGGATATGCCTCCAG; GTTAAATCCTGTTTTGAATATACTGCTAACCCCGCTGGTTATAATCCTTCAATATCAATT; GTGGGCACACTTGAAGCTGAAAAAGAAAGAAGAAAATCTGGGCTATCCTCAAGAGTTCAG; TTTCGAAACCAAGGTTCTGAGCCCAAATATACTCAAGAACTAACTCTGAAGAGGCAGAAA; CAGAAAGTGTGCATGGAGGAAACCCTGTGGCTACAGGATAATATCAGAGATAAACTGCGT; CCCATTCCCATAACTGCCTCAGTGGAGATCCAAGAGCCAAGCTCTCGTAGGCGAGTGAAT; TCACTTCCAGAAGTTCTTCCAATTCTGAATTCAGATGAACCCAAGACAGCTCATATTGAT; GTTCACTTCTTAAAAGAGGGATGTGGAGACGACAATGTATGTAACAGCAACCTTAAACTA; GAATATAAATTTTGCACCCGAGAAGGAAATCAAGACAAATTTTCTTATTTACCAATTCAA; AAAGGTGTACCAGAACTAGTTCTAAAAGATCAGAAGGATATTGCTTTAGAAATAACAGTG; ACAAACAGCCCTTCCAACCCAAGGAATCCCACAAAAGATGGCGATGACGCCCATGAGGCT; AAACTGATTGCAACGTTTCCAGACACTTTAACCTATTCTGCATATAGAGAACTGAGGGCT; TTCCCTGAGAAACAGTTGAGTTGTGTTGCCAACCAGAATGGCTCGCAAGCTGACTGTGAG; CTCGGAAATCCTTTTAAAAGAAATTCAAATGTCACTTTTTATTTGGTTTTAAGTACAACT; GAAGTCACCTTTGACACCCCAGATCTGGATATTAATCTGAAGTTAGAAACAACAAGCAAT; CAAGATAATTTGGCTCCAATTACAGCTAAAGCAAAAGTGGTTATTGAACTGCTTTTATCG; GTCTCGGGAGTTGCTAAACCTTCCCAGGTGTATTTTGGAGGTACAGTTGTTGGCGAGCAA; GCTATGAAATCTGAAGATGAAGTGGGAAGTTTAATAGAGTATGAATTCAGGGTAATAAAC; TTAGGTAAACCTCTTACAAACCTCGGCACAGCAACCTTGAACATTCAGTGGCCAAAAGAA; ATTAGCAATGGGAAATGGTTGCTTTATTTGGTGAAAGTAGAATCCAAAGGATTGGAAAAG; GTAACTTGTGAGCCACAAAAGGAGATAAACTCCCTGAACCTAACGGAGTCTCACAACTCA; AGAAAGAAACGGGAAATTACTGAAAAACAGATAGATGATAACAGAAAATTTTCTTTATTT; GCTGAAAGAAAATACCAGACTCTTAACTGTAGCGTGAACGTGAACTGTGTGAACATCAGA; TGCCCGCTGCGGGGGCTGGACAGCAAGGCGTCTCTTATTTTGCGCTCGAGGTTATGGAAC; AGCACATTTCTAGAGGAATATTCCAAACTGAACTACTTGGACATTCTCATGCGAGCCTTC; ATTGATGTGACTGCTGCTGCCGAAAATATCAGGCTGCCAAATGCAGGCACTCAGGTTCGA; GTGACTGTGTTTCCCTCAAAGACTGTAGCTCAGTATTCGGGAGTACCTTGGTGGATCATC; CTAGTGGCTATTCTCGCTGGGATCTTGATGCTTGCTTTATTAGTGTTTATACTATGGAAG; TGTGGTTTCTTCAAGAGAAATAAGAAAGATCATTATGATGCCACATATCACAAGGCTGAG; ATCCATGCTCAGCCATCTGATAAAGAGAGGCTTACTTCTGATGCATAGTATTGATCTACT; TCTGTAATTGTGTGGATTCTTTAAACGCTCTAGGTACGATGACAGTGTTCCCCGATACCA; TGCTGTAAGGATCCGGAAAGAAGAGCGAGAGATCAAAGATGAAAAGTATATTGATAACCT; TGAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000210 unedited; TGTGCGAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGATTCGGCACCAGGCCGCT; GCAGGTCCCCGCTCCCCTCCCCGTGCGTCCGCCCATGGCCGCCGCCGGGCAGCTGTGCTT; GCTCTACCTGTCACTCGGGGCTCCTGTCCCGGCTCGGCGCAGCCTTCAACTTGGACACTC; GGGAGGACAACGTGATCCGGAAATATGGAGACCCCGGGAGCCTCTTCGGCTTCTCGCTGG; CCATGCACTGGCAACTGCAGCCCGAGGACAAGCGGCTGTTGCTCGTGGGGGCCCCGCGGG; CAGAAGCGCTTCCACTGCAGAGAGCCAACAGAACGGGAGGGCTGTACAGCTGCGACACTA; CCGCCCGGGGGCTCATGCACGCGGATCGAGTTTGATAACGATGCTGACCCCACGTCAGAA; AGCAAGGAAGATCAGTGGATGGGGGTCACCGTCCAGAGCCAAGGTCCAGGGGGCAAGGTC; GTGACATGTGCTCACCGATATGAAAAAAGGCAGCATGTTAATACGAAGCAGGAATCCCGA; GACATCTTTGGGCGGTGTTATGTCCTGAGTCAGAATCTCAGGATTGAAGACGATATGGAT; GGGGGAGATTGGAGCTTTTGTGATGGGCGATTGAGAGGCCATGAGAAATTTGGCTCTTGC; CAGCAAGGTGTAACAGCTACTTTTACTAAAGACTTTCATTACATTGTATTTGGAGCCCCG; GTACTTACTACTGGAAAGGGATGTTCGTGTAGAGCACAAGAATACCACTTTTTTGCCATG; AACATCTTTTGAGATGGGCCTTATGAATTTGGTGGAGAGACTGAGCATGATGAAAGTCTC; GGTCCTGTTCCTGCTAACAGTACTTAGGTTTTCCTTGCACTCAGGCAAGGCATTGTTTCT; AAAGATGAGACACCTTTTGAATCTGGGGCTCCCAGAACCCATCCAGTGCAGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000210 unedited; NAACCCCGGGAANAAACCCCCCAACCATCCCCCAAAATNNTTGACTGTGCNCNCCGGCCC; GCTATTCTNANGATCGNGTTTTTTTTTTTTTTTTTTTAACTTTTATAAAAAATTATTTCA; CACTTTTATACAAATATCCACAGGTAATTTCTAATCATGAAATAATTTTACAATGCTAGT; TAGTACAAAAATGTATCAAATGCAAACAGACCAGTGACTTGACTTGAGATGACATTTTTA; ACAACCATAAATCTAAACACCATAACATCTTTGTTTCTTTGTTGAACCACCCTCCCAACA; CCCTTTTCAGGACATTCATCTTTTTTTTAGCAACACAGGTCTAAAAACACTGTCAAAGTA; TCCAGTTCCTTACCAATCTACTAATCTACACCACATTAGGGACACAAGCTCCCCCATCAA; GAAACTGACACTTCAAACACTGTTATAACCTGTTTTACCGCACCTCTCGCAAACCATCTA; CCTTTCCCGACTAATACTTCTACCCTTCTACCCAAACAACTCCCTTTGCTTCTTACCGCT; TTTCATTCCCCGAAATTATAAACCCCTTTCTCACTTACCCCTTGCCTCTCTTCCCGCTCC; CCTCCCCTTCCCATGTTCTCCCCCACGTCCTCACCACACCCAATTATTCCCCCCATTATT; TCGCCCTTTCCCCCCCACTCCCCCCCCCCTCTTCCCCCCCTTTCCCTCACCCCCCCCCCC; CCTTTTCCTCCTATTTTCCCAATTTCCCTCCCTTCCCCTATTTTTCCCCCTCCCTTCCTC; CGCTTCCCTTCCCCGATTCCCCTCTTCGTCTCCATCCCTCACCCTCTACCACCTCTTCCA; CCACCCCCCTCGCTCTTCACCCACCTATCTTCTCCCTCCCTCCCCCCCTCCTCCCTTCTC; CTTCCCCCCTCCCCCTCCCTCCTCCCTTATTTCCCCTTCCCTTTTTTTCCCCTTCTCCTT; CGCCCTCCTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000210
• Insert Size: 4730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000210.1, NP_000201.1
• RefSeq Size: 5611 bp
• RefSeq ORF: 3222 bp
• Locus ID: 3655
• UniProt ID: P23229
• Cytogenetics: 2q31.1
• Domains: FG-GAP
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer
• Summary: The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 6 subunit. This subunit may associate with a beta 1 or beta 4 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. The alpha 6 beta 4 integrin may promote tumorigenesis, while the alpha 6 beta 1 integrin may negatively regulate erbB2/HER2 signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]; Transcript Variant: This variant (2) contains an alternate coding exon compared to variant 1, which results in a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a.