PGK1 (NM_000291) Human Untagged Clone

SKU SC119988

PGK1 (untagged)-Human phosphoglycerate kinase 1 (PGK1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PGK1
• Synonyms: HEL-S-68p; MIG10; PGKA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119988 sequence for NM_000291 edited (data generated by NextGen Sequencing); ATGTCGCTTTCTAACAAGCTGACGCTGGACAAGCTGGACGTTAAAGGGAAGCGGGTCGTT; ATGAGAGTCGACTTCAATGTTCCTATGAAGAACAACCAGATAACAAACAACCAGAGGATT; AAGGCTGCTGTCCCAAGCATCAAATTCTGCTTGGACAATGGAGCCAAGTCGGTAGTCCTT; ATGAGCCACCTAGGCCGGCCTGATGGTGTGCCCATGCCTGACAAGTACTCCTTAGAGCCA; GTTGCTGTAGAACTCAAATCTCTGCTGGGCAAGGATGTTCTGTTCTTGAAGGACTGTGTA; GGCCCAGAAGTGGAGAAAGCCTGTGCCAACCCAGCTGCTGGGTCTGTCATCCTGCTGGAG; AACCTCCGCTTTCATGTGGAGGAAGAAGGGAAGGGAAAAGATGCTTCTGGGAACAAGGTT; AAAGCCGAGCCAGCCAAAATAGAAGCTTTCCGAGCTTCACTTTCCAAGCTAGGGGATGTC; TATGTCAATGATGCTTTTGGCACTGCTCACAGAGCCCACAGCTCCATGGTAGGAGTCAAT; CTGCCACAGAAGGCTGGTGGGTTTTTGATGAAGAAGGAGCTGAACTACTTTGCAAAGGCC; TTGGAGAGCCCAGAGCGACCCTTCCTGGCCATCCTGGGCGGAGCTAAAGTTGCAGACAAG; ATCCAGCTCATCAATAATATGCTGGACAAAGTCAATGAGATGATTATTGGTGGTGGAATG; GCTTTTACCTTCCTTAAGGTGCTCAACAACATGGAGATTGGCACTTCTCTGTTTGATGAA; GAGGGAGCCAAGATTGTCAAAGACCTAATGTCCAAAGCTGAGAAGAATGGTGTGAAGATT; ACCTTGCCTGTTGACTTTGTCACTGCTGACAAGTTTGATGAGAATGCCAAGACTGGCCAA; GCCACTGTGGCTTCTGGCATACCTGCTGGCTGGATGGGCTTGGACTGTGGTCCTGAAAGC; AGCAAGAAGTATGCTGAGGCTGTCACTCGGGCTAAGCAGATTGTGTGGAATGGTCCTGTG; GGGGTATTTGAATGGGAAGCTTTTGCCCGGGGAACCAAAGCTCTCATGGATGAGGTGGTG; AAAGCCACTTCTAGGGGCTGCATCACCATCATAGGTGGTGGAGACACTGCCACTTGCTGT; GCCAAATGGAACACGGAGGATAAAGTCAGCCATGTGAGCACTGGGGGTGGTGCCAGTTTG; GAGCTCCTGGAAGGTAAAGTCCTTCCTGGGGTGGATGCTCTCAGCAATATTTAG; ; Clone variation with respect to NM_000291.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000291 unedited; GTTAGGAATTGTATCCGACTCCTATAGGCGGCCGCGCAATTCGCACGAGGCCGCATTCTG; CAAGCCTCCGGAGCGCACGTCGGCAGTCGGCTCCCTCGTTGACCGAATCACCGACCTCTC; TCCCCAGCTGTATTTCCAAAATGTCGCTTTCTAACAAGCTGACGCTGGACAAGCTGGACG; TTAAAGGGAAGCGGGTCGTTATGAGAGTCGACTTCAATGTTCCTATGAAGAACAACCAGA; TAACAAACAACCAGAGGATTAAGGCTGCTGTCCCAAGCATCAAATTCTGCTTGGACAATG; GAGCCAAGTCGGTAGTCCTTATGAGCCACCTAGGCCGGCCTGATGGTGTGCCCATGCCTG; ACAAGTACTCCTTAGAGCCAGTTGCTGTAGAACTCAAATCTCTGCTGGGCAAGGATGTTC; TGTTCTTGAAGGACTGTGTAGGCCCAGAAGTGGAGAAAGCCTGTCCCAACCCAGCTGCNT; GGGTCTGTCATCCTGCTGGAGAACCTCCGCTTTCATGTGGAGGAAGAAGGGAAGGGAAAA; GATGCTTCTGGGAACAAGGTTAAAGCCGAGCCAGCCAAAATAGAAGCTTTCCGAGCTTCA; CTTTCCAAGCNTAGGGGATGTCTATGTCAATGATGCTTTTGGCACTGCTCACAGAGCCCA; CAGCTCCATGGTAGGAGTCAATCTGCCACAGAAGGCTGGTGGGTTNTTGATGAAGAAGGA; GCTGAACTACTTTGNCAAGGCCTTGGAGAGCCCAGAGCGACCCTTCCTGGCCATCCTGNG; CGGAGCTAAAGNTGCAGACCAGATCCAGCTCATCAATAATATGCTGGACAAAGTCAATGA; GATGATTATTGGTGGNTGGAAATGGCTTTACCTTCCTTAANNGTGCTCACACATGGAGAT; GCCCTTTNCCTTGTTTGATGAAAAGGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000291 unedited; ATGGCACTTCCAGGGCCAGGTANAGCACTGGTGGNAGGGGTCACAGGGNATGCCCACCCG; GGTATCTGGTTCAGGNAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTT; TTTTTTTTTTCACGGTTTCAATGGACACTTTTATTGTTTACTTAATGGATCATCAATTTT; GTCTCACTACCTACAAATGGAATTTCATCTTGTTTCCATGCTGAGTAGTGAAACAGTGAC; AAAGCTAATCATAATAACCTACATCAAAAGAGAACTAAGCTAACACTGCTCACTTTCTTT; TTAACAGGCAAAATATAAATATATGCACTCTAGAATGCACAATGGTTTATTCACTAAAAA; ATTCAAATGGGATCTTGAAGAATGTATGCAAATCCAGGGTGCAGAGAAAATGAGCTGATA; TGCTGTGCAACTGTTTAAGGGTTCCTGGCACTGCATCTCTTGGCCACTAGCTGAATCTTG; ACATGGAAGGTTTTAGCTAATGCCAAGTGGAGATGCAGAAAATGCTAAGTTGACTTAGGG; GCTGTGCACAGGAACTAAAAGGCAGGAAAGTACTAAATATTGCTGAGAGCATCCACCCCA; GGAAGGACTTTACCTTCCAGGAGCTCCAAACTGGCACCACCCCCAGTGCTCACATGGCTG; ACTTTATCCTCCGTGTTCCATTTGGCACAGCAAGTGGCAGTGTCTCCACCACCTATGATG; GTGATGCAGCCCCTANAAGTGGCTTTTCACACCTCATCCATGAGAAGCTTTGGTTCCCCG; GGCAAAAGCTTTCCATTCAAATACCCNNCACAGGACCATTCCACACAAATCTGCTTAGNC; CCCGATGACAGNCCTNAGCATACTTTCTTGCTGCTTTTCAGAACCACAAGTCCAAGCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000291
• Insert Size: 1910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000291.2, NP_000282.1
• RefSeq Size: 2338 bp
• RefSeq ORF: 1254 bp
• Locus ID: 5230
• UniProt ID: P00558
• Cytogenetics: Xq21.1
• Domains: PGK
• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways
• Summary: The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]