PEPD (NM_000285) Human Untagged Clone

SKU SC119982

PEPD (untagged)-Human peptidase D (PEPD), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PEPD
• Synonyms: PROLIDASE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000285, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAACCCTGAAGGTGCCGCTGGCGCTCTTTG; CCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGCGGAAGAACCCTGCTGTGCAGGCCGGCTCCATCGTGGT; CCTGCAGGGCGGGGAGGAGACTCAGCGCTACTGCACCGACACCGGGGTCCTCTTCCGCCAGGAGTCCTTC; TTTCACTGGGCGTTCGGTGTCACTGAGCCAGGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGA; CCCTGTTTGTGCCCAGGCTTCCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGGAGCACTT; CAAGGAGAAGTATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCGTCCTGACGTCACAGAAG; CCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCGGCAGTGTCTGCAGGGAGGCCTCCTTTG; ACGGCATCAGCAAGTTCGAAGTCAACAATACCATTCTTCACCCAGAGATCGTTGAGTGCCGAGTGTTTAA; GACGGATATGGAGCTGGAGGTTCTGCGCTATACCAATAAAATCTCCAGCGAGGCCCACCGTGAGGTAATG; AAGGCTGTAAAAGTGGGAATGAAAGAATATGAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCCGGG; GCGGCATGCGCCACAGCTCCTACACCTGCATCTGCGGCAGTGGTGAGAACTCAGCCGTGCTACACTACGG; ACACGCCGGAGCTCCCAACGACCGAACGATCCAGAATGGGGATATGTGCCTGTTCGACATGGGCGGTGAG; TATTACTGCTTCGCTTCCGACATCACCTGCTCCTTTCCCGCCAACGGCAAGTTCACTGCAGACCAGAAGG; CCGTCTATGAGGCAGTGCTGCGGAGCTCCCGTGCCGTCATGGGTGCCATGAAGCCAGGTGTCTGGTGGCC; TGACATGCACCGCCTGGCTGACCGCATCCACCTGGAGGAGCTGGCCCACATGGGCATCCTGAGCGGCAGC; GTGGACGCCATGGTCCAGGCTCACCTGGGGGCCGTGTTTATGCCTCACGGGCTTGGCCACTTCCTGGGCA; TTGACGTGCACGACGTGGGAGGCTACCCAGAGGGCGTGGAGCGCATCGACGAGCCCGGCCTGCGGAGCCT; GCGCACTGCACGGCACCTGCAGCCAGGCATGGTGCTCACCGTGGAGCCGGGCATCTACTTCATCGACCAC; CTCCTGGATGAGGCCCTGGCGGACCCGGCCCGCGCCTCCTTCCTTAACCGCGAGGTCCTGCAGCGCTTTC; GCGGTTTTGGCGGGGTCCGCATCGAGGAGGACGTCGTGGTGACTGACAGCGGCATAGAGCTGCTGACCTG; CGTGCCCCGCACTGTGGAAGAGATTGAAGCATGCATGGCAGGCTGTGACAAGGCCTTTACCCCCTTCTCT; GGCCCCAAGTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000285 unedited; ATTTTTCANATTTTGTATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGTGA; CGCCGGTGCCGGGCGAACTGGCGGCGGCCACCGGACCCTCGTTTTGGCTGGGGAATGAAA; CCCTGAAGGTGCCGCTGGCGCTCTTTGCCTTGAACCGGCAGCGCCTGTGTGAGCGGCTGC; GGAAGAACCCTGCTGTGCAGGCCGGCTCCATCGTGGTCCTGCAGGGCGGGGAGGAGACTC; AGCGCTACTGCACCGACACCGGGGTCCTCTTCCGCCAGGAGTCCTTCTTTCACTGGGCGT; TCGGTGTCACTGAGCCAGGCTGCTATGGTGTCATCGATGTTGACACTGGGAAGTCGACCC; TGTTTGTGCCCAGGCTTCCTGCCAGCCATGCCACCTGGATGGGAAAGATCCATTCCAAGG; AGCACTTCAAGGAGAAGTATGCCGTGGACGACGTCCAGTACGTAGATGAGATTGCCAGCG; TCCTGACGTCACAGAAGCCCTCTGTCCTCCTCACTTTGCGTGGCGTCAACACGGACAGCG; GCAGTGTCTGCAGGGAGGCCTCCTTTGACGGCATCAGCAAGTTCGAAGTCAACAATACCA; TTCTTCACCCAGAGATCGTTGAGTGCCGAGTGTTTAAGACGGATATGGAGCTGGAGGTTC; TGCGCTATACCAATAAAATCTCCAGCGAGGCCCACCGTGAGGTAATGAAGGCTGTAAAAG; TGGGAATGAAAGAATATGAGTTGGAAAGCCTCTTCGAGCACTACTGCTACTCCNGGNGCG; GCATGCGCCACAGCTCCTACACCTGCATCTGCGGCAGTGGTGAGACNTCAGCCGTGCTAC; ACTACGGACACGCCGGAGCTNCCAACGACCGAACGATTCAGAATGGGGATATGTGCCCTG; TTCGACATG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000285 unedited; TTGGACCGCGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTGACCATTTTAGGAAA; CCTTTTATTGCAAATGCCATTCTGCATATTGATTTTTGACAGAAAGTATCAGAAATGCTT; CTTTCCTGGGAAAAGGAATATAAATGACAGCAAGACACATTTTAGTTGCTACTAAAGAAC; AGCATTATTTTCAATCATTTTAAGTCGCTCATTTAATAAGCAAGGTATAAAACAGATTAA; AGGTGGGAGCCTGCAAAAGGGTAATTAAAAAAGTGTTTCCTCCCCGGGAAACAGCACTGT; TTGGTCTGATCAAATGCCGAAGCTGGGATCTGATTCTGGGTGCCGTCTCTCGCTACTGGA; GTGCTGACCAGCAGGCTGCCCATCACGAAAAGAGGTTGCAAGGCCAGGCCCCCAGGTGCG; CTGGGATTTCTGGCTGGCTCTACTTGGGGCCAGAGAAGGGGGTAAAGGCCTTGTCACAGC; CTGCCATGCATGCTTCAATCTCTTCCACAGTGCGGGGCACGCAGGTCAGCAGCTCTATGC; CGCTGTCAGTCACCACGACGTCCTCCTCGATGCGGACCCCGCCAAAACCGCGAAAGCGCT; GCAGGACCTCGCGGTTAAGGAAGGAGGCGCGGGCCGGGTCCGCCAGGGCCTCATCCAGGA; GGTGGTCGATGAAGTAGATGCCCGGCTCCACAGTGAGCACCATGCCTGGCTGCANGTGCC; GTGCANTGCGCANGCTCCGCAGGCCCGGCTCGTCGATGCGCTCCACGCCCTCTGGGTAGC; CTCCCCCTCATGCCCGTAATGCCAGGAAGTGGCCAACCCGTGAAGCATAAACCCGCGCCC; AGGGAACCCTGACCCTGGCGTTCACCCTGCCTCAAGATGGCCATGTGGGCCAACTCCTTC; AAGGGGAGCGGTAANCCAGCCGGGCTGTAAGGCCCCCAACCCTGGCTTTTGCCCCCCTGA; CGGCCGGAGCTCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000285
• Insert Size: 1910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000285.1, NP_000276.1
• RefSeq Size: 1888 bp
• RefSeq ORF: 1482 bp
• Locus ID: 5184
• UniProt ID: P12955
• Cytogenetics: 19q13.11
• Domains: AMP_N, Peptidase_M24
• Protein Families: Druggable Genome, Protease
• Summary: This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).