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UMPS (NM_000373) Human Untagged Clone

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SKU
SC119938
UMPS (untagged)-Human uridine monophosphate synthetase (UMPS), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol UMPS
Synonyms OPRT
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC119938 sequence for NM_000373 edited (data generated by NextGen Sequencing)
ATGGCGGTCGCTCGTGCAGCTTTGGGGCCATTGGTGACGGGTCTGTACGACGTGCAGGCT
TTCAAGTTTGGGGACTTCGTGCTGAAGAGCGGGCTTTCCTCCCCCATCTACATCGATCTG
CGGGGCATCGTGTCTCGACCGCGTCTTCTGAGTCAGGTTGCAGATATTTTATTCCAAACT
GCCCAAAATGCAGGCATCAGTTTTGACACCGTGTGTGGAGTGCCTTATACAGCTTTGCCA
TTGGCTACAGTTATCTGTTCAACCAATCAAATTCCAATGCTTATTAGAAGGAAAGAAACA
AAGGATTATGGAACTAAGCGTCTTGTAGAAGGAACTATTAATCCAGGAGAAACCTGTTTA
ATCATTGAAGATGTTGTCACCAGTGGATCTAGTGTTTTGGAAACTGTTGAGGTTCTTCAG
AAGGAGGGCTTGAAGGTCACTGATGCCATAGTGCTGTTGGACAGAGAGCAGGGAGGCAAG
GACAAGTTGCAGGCGCACGGGATCCGCCTCCACTCAGTGTGTACATTGTCCAAAATGCTG
GAGATTCTCGAGCAGCAGAAAAAAGTTGATGCTGAGACAGTTGGGAGAGTGAAGAGGTTT
ATTCAGGAGAATGTCTTTGTGGCAGCGAATCATAATGGTTCTCCCCTTTCTATAAAGGAA
GCACCCAAAGAACTCAGCTTCGGTGCACGTGCAGAGCTGCCCAGGATCCACCCAGTTGCA
TCGAAGCTTCTCAGGCTTATGCAAAAGAAGGAGACCAATCTGTGTCTATCTGCTGATGTT
TCACTGGCCAGAGAGCTGTTGCAGCTAGCAGATGCTTTAGGACCTAGTATCTGCATGCTG
AAGACTCATGTAGATATTTTGAATGATTTTACTCTGGATGTGATGAAGGAGTTGATAACT
CTGGCAAAATGCCATGAGTTCTTGATATTTGAAGACCGGAAGTTTGCAGATATAGGAAAC
ACAGTGAAAAAGCAGTATGAAGGAGGTATCTTTAAAATAGCTTCCTGGGCAGATCTAGTA
AATGCTCACGTGGTGCCAGGCTCAGGAGTTGTGAAAGGCCTGCAAGAAGTGGGCCTGCCT
TTGCATCGGGGGTGCCTCCTTATTGCGGAAATGAGCTCCACCGGCTCCCTGGCCACTGGG
GACTACACTAGAGCAGCGGTTAGAATGGCTGAGGAGCACTCTGAATTTGTTGTTGGTTTT
ATTTCTGGCTCCCGAGTAAGCATGAAACCAGAATTTCTTCACTTGACTCCAGGAGTTCAG
TTGGAAGCAGGAGGAGATAATCTTGGCCAACAGTACAATAGCCCACAAGAAGTTATTGGC
AAACGAGGTTCCGATATCATCATTGTAGGTCGTGGCATAATCTCAGCAGCTGATCGTCTG
GAAGCAGCAGAGATGTACAGAAAAGCTGCTTGGGAAGCGTATTTGAGTAGACTTGGTGTT
TGA

Clone variation with respect to NM_000373.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000373 unedited
GTCAGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGCCTCGTGCCGA
ATTCGGCACGAGGAGGCAGCGCGCGACAATGGCGGTCGCTCGTGCAGCTTTGGGGCCATT
GGTGACGGGTCTGTACGACGTGCAGGCTTTCAAGTTTGGGGACTTCGTGCTGAAGAGCGG
GCTTTCCTCCCCCATCTACATCGATCTGCGGGGCATCGTGTCTCGACCGCGTCTTCTGAG
TCAGGTTGCAGATATTTTATTCCAAACTGCCCAAAATGCAGGCATCAGTTTTGACACCGT
GTGTGGAGTGCCTTATACAGCTTTGCCATTGGCTACAGTTATCTGTTCAACCAATCAAAT
TCCAATGCTTATTAGAAGGAAAGAAACAAAGGATTATGGAACTAAGCGTCTTGTAGAAGG
AACTATTAATCCAGGAGAAACCTGTTTAATCATTGAAGATGTTGTCACCAGTGGATCTAG
TGTTTTGGAAACTGTTGAGGTTCTTCAGAAGCTGGGCTTGAAGGTCACTGATGCCATAGT
GCTGTTGGACAGAGAGCAGGGAGGCAAGGACAAGTTGCAGGCGCACGGGATCCGCCTCCA
CTCAGTGTGTACATTGTCCAAAATGCTGGAGATTCTCGAGCAGCAGAAAAAAGTTGATGC
TGAGACAGTTGGGAGAGTGAAGAGGTTTATTCAGGAGAATGTCTTTGTGCCAGCGAATCA
TAATGGTTCTCCCCTTTCTATAAAGGAAGCACCCCAAAGAACTCAGCTTTCGTTGCACGT
GCCAAAGCTGCCCAGGGATCCACCCCAGTTGCATCGAAAGCTTCTCAAGCTTATGCAAAA
GGAAGGAGACCAATCTGGGNCTATCTGCTGATGTTTCACTGGCCAGAGNACTGNTGCAGC
TAACAGATGCTTTAGACA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000373 unedited
GGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCAAGCTTAAAAAT
GAACTTATTATAGTATCAATGCGGTGATTACAAATTACTCAATATTTCCTAAAGACCATG
AGAACTCCAGAACCCATTCTTACACAGGCCCTGTGGAAGAATCCAAGCAGGAATAATTGG
ATTATTTCCAGCCAGTGACTTTCAGGGGACCACATATCTTCAATGTCTTCACATTGTATC
TGAAAAATGTATCTGAAGCACTCAAACACCAAGTCTACTCAAATACGCTTCCCAAGCAGC
TTTTCTGTACATCTCTGCTGCTTCCAGACGATCAGCTGCTGAGATTATGCCACGACCTAC
AATGATGATATCGGAACCTCGTTTGCCAATAACTTCTTGTGGGCTATTGTACTGTTGGCC
AAGATTATCTCCTCCTGCTTCCAACTGAACTCCTGGAGTCAAGTGAAGAAATTCTGGTTT
CATGCTTACTCGGGAGCCAGAAATAAAACCAACAACAAATTCAGAGTGCTCCTCAGCCAT
TCTAACCGCTGCTCTAGTGTAGTCCCCAGTGGCCAGGGAGCCGGTGGAGCTCATTTCCGC
AATAAGGAGGCACCCCCGATGCAAAGGCAGGCCCACTTCTTGCAGGCCTTTCACAACTCC
TGAGCCTGGCACCACGTGAGCATTTACTAGATCTGCCCAGGAAGCTATTTTAAAGATACC
TCCTAAGAAGAAGGGGGAAAAAAGATATTTGCTGATTTTCAACAAAACCTCTAACCCCTC
TGTCCACCAACCAACTANCATATGAAATNAATCTCCAGTNACATGTTAATGGAGAGATCA
AAACTCTTCAGGAACTTNTAGTGATATCAGCAGACCCATGGTTCCATCTGTGAAACTGTA
TCTGTTATTCAGGTAAGTANCTGGGTGAN
Restriction Sites NotI-NotI |
ACCN NM_000373
Insert Size 3630 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000373.1, NP_000364.1
RefSeq Size 2244 bp
RefSeq ORF 1443 bp
Locus ID 7372
UniProt ID P11172
Cytogenetics 3q21.2
Domains OMPdecase, Pribosyltran
Protein Families Druggable Genome
Protein Pathways Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism
Summary This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. provided by RefSeq, Mar 2010
Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_000373" in other vectors (6)
SKU Description Size Price
RC201324 UMPS (Myc-DDK-tagged)-Human uridine monophosphate synthetase (UMPS), transcript variant 1 10 ug
$457.00
RC201324L1 Lenti ORF clone of Human uridine monophosphate synthetase (UMPS), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201324L2 Lenti ORF clone of Human uridine monophosphate synthetase (UMPS), transcript variant 1, mGFP tagged 10 ug
$757.00
RC201324L3 Lenti ORF clone of Human uridine monophosphate synthetase (UMPS), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201324L4 Lenti ORF clone of Human uridine monophosphate synthetase (UMPS), transcript variant 1, mGFP tagged 10 ug
$757.00
RG201324 UMPS (tGFP-tagged) - Human uridine monophosphate synthetase (UMPS), transcript variant 1 10 ug
$489.00 $657.00

file-alt Citations

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