APRT (NM_000485) Human Untagged Clone

SKU SC119854

APRT (untagged)-Human adenine phosphoribosyltransferase (APRT), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: APRT
• Synonyms: AMP; APRTD
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000485, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCGACTCCGAGCTGCAGCTGGTTGAGCAGCGGATCCGCAGCTTCCCCGACTTCCCCACCCCAGGCG; TGGTATTCAGGGACATCTCGCCCGTCCTGAAGGACCCCGCCTCCTTCCGCGCCGCCATCGGCCTCCTGGC; GCGACACCTGAAGGCGACCCACGGGGGCCGCATCGACTACATCGCAGGCCTAGACTCCCGAGGCTTCCTC; TTTGGCCCCTCCCTGGCCCAGGAGCTTGGACTGGGCTGCGTGCTCATCCGAAAGCGGGGGAAGCTGCCAG; GCCCCACTCTGTGGGCCTCCTATTCCCTGGAGTACGGGAAGGCTGAGCTGGAGATTCAGAAAGACGCCCT; GGAGCCAGGACAGAGGGTGGTCGTCGTGGATGATCTGCTGGCCACTGGTGGAACCATGAACGCTGCCTGT; GAGCTGCTGGGCCGCCTGCAGGCTGAGGTCCTGGAGTGCGTGAGCCTGGTGGAGCTGACCTCGCTTAAGG; GCAGGGAGAAGCTGGCACCTGTACCCTTCTTCTCTCTCCTGCAGTATGAGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000485 unedited; GCGCCCCGTTGCCGCATATGGGCGGTAGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC; TCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTC; GGCACGAGGGGGCTGCCGCTGGCTCTTCGCACGCGGCCATGGCCGACTCCGAGCTGCAGC; TGGTTGAGCAGCGGATCCGCAGCTTCCCCGACTTCCCCACCCCAGGCGTGGTATTCAGGG; ACATCTCGCCCGTCCTGAAGGACCCCGCCTCCTTCCGCGCCGCCATCGGCCTCCTGGCGC; GACACCTGAAGGCGACCCACGGGGGCCGCATCGACTACATCGCAGGCTGAGCTGGAGATT; CAGAAAGACGCCCTGGAGCCAGGACAGAGGGTGGTCGTCGTGGATGATCTGCTGGCCACT; GGTGGAACCATGAACGCTGCCTGTGAGCTGCTGGGCCGCCTGCAGGCTGAGGTCCTGGAG; TGCGTGAGCCTGGTGGAGCTGACCTCGCTTAAGGGCAGGGAGAAGCTGGCACCTGTACCC; TTCTTCTCTCTCCTGCAGTATGAGTGACCACAGGGCCTCCCAGCCCAACATCTCCAGCTG; GATCCCAGGGAAATATCAGCCTTGGGCAACTGCAGTGACCAGGGGCACCGGCTGCCCACA; GGGAACACATTCCTTTGCTGGGGTTCAGCGCCTCTCCTGNGGCTGGAAGTGCCAAAGCCT; GGGGCANAGCTGTGTTTCAGCCACACTGAACCCAATTACACACAGCGGGAGAACGCAGTA; NACAGCTTTCCCNACAAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCG; GTCATAGCTGTTNCCCTGACAGATCCCGGGTGGGCATCCTGTGACCCCTCCCAGTGCCTC; TCCTGGCCCTGNNANNNTGCACTCAGTGCCCACCACCNTNNGTCTATAAANTAAGTGCAT; CATTTGTCTGACTAGTGTCCTCATAATATATGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000485 unedited; TTAGTTTTAAAACCTTTTTTTTTTTTTTTTGGGGGGATTTCTGTTTACTGCGTTCTCCCG; CTGTGTGTAATTGGGTTCAGTGTGGCTGAAACACAGCTTTGCCCCAGGCTTTGGCACTTC; CAGCCCCAGGAGAGGCGCTGAACCCCAGCAAAGGAATGTGTTCCCTGTGGGCAGCCGGTG; CCCCTGGTCACTGCAGTTGCCCAAGGCTGATATTTCCCTGGGATCCAGCTGGAGATGTTG; GGCTGGGAGGCCCTGTGGTCACTCATACTGCAGGAGAGAGAAGAAGGGTACAGGTGCCAG; CTTCTCCCTGCCCTTAAGCGAGGTCAGCTCCACCAGGCTCACGCACTCCAGGACCTCAGC; CTGCAGGCGGCCCAGCAGCTCACAGGCAGCGTTCATGGTTCCACCAGTGGCCAGCAGATC; ATCCACGACGACCACCCTCTGTCCTGGCTCCAGGGCGTCTTTCTGAATCTCCAGCTCAGC; CTGCGATGTAGTCGATGCGGCCCCCGTGGGTCGCCTTCAGGTGTCGCGCCAGGAGGCCGA; TGGCGGCGCGGAAAGAGGCGGNGTCCTTCAGGACGGGCGAGATGTCCCTGAATACCACGC; CTGGGGTGGGGAAGTCGGGGAAGCTGCGGATCCGCTGCTCAACCAGCTGCAGCTCGGAGT; CGGCCATGGGCGCGTGCGAAGAGCCAGCGGCAGCCCCCCTCGTGCCGAATTCGCGGCCGC; TGCANAAAGAACAGTAGCCTGTATTCTATAGTGTCACCTAAATGAGCTCTGGCTATATAG; ACCTNCCACCGTACACGCCTTACCGCCCATTTGCGGTCACGGGGNCGGGGGTATTACGAC; ATTTTGGAAAGTCCCGGTGATTTTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_000485
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000485.2, NP_000476.1
• RefSeq Size: 807 bp
• RefSeq ORF: 543 bp
• Locus ID: 353
• UniProt ID: P07741
• Cytogenetics: 16q24.3
• Domains: Pribosyltran
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• Summary: Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).