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Complement C8A (C8A) (NM_000562) Human Untagged Clone

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SKU
SC119839
C8A (untagged)-Human complement component 8, alpha polypeptide (C8A)
  $545.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Complement C8A
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC119839 sequence for NM_000562 edited (data generated by NextGen Sequencing)
ATGTTTGCTGTTGTTTTCTTCATCTTGTCTTTGATGACTTGTCAGCCTGGGGTAACTGCA
CAGGAGAAGGTGAACCAGAGAGTAAGACGGGCAGCTACACCCGCAGCAGTTACCTGCCAG
CTGAGCAACTGGTCAGAGTGGACAGATTGCTTTCCGTGCCAGGACAAAAAGTACCGACAC
CGGAGCCTCTTGCAGCCAAACAAGTTTGGGGGAACCATCTGCAGTGGTGACATCTGGGAT
CAAGCCAGCTGCTCCAGTTCTACAACTTGTGTAAGGCAAGCACAGTGTGGACAGGATTTC
CAGTGTAAGGAGACAGGTCGCTGCCTGAAACGCCACCTTGTGTGTAATGGAGACCAGGAC
TGCCTTGATGGCTCTGATGAGGACGACTGTGAAGATGTCAGGGCCATTGACGAAGACTGC
AGCCAGTATGAACCAATTCCAGGATCACAGAAGGCAGCCTTGGGGTACAATATCCTGACC
CAGGAAGATGCTCAGAGTGTGTACGATGCCAGTTATTATGGGGGCCAGTGTGAGACGGTA
TACAATGGGGAATGGAGGGAGCTTCGATATGACTCCACCTGTGAACGTCTCTACTATGGA
GATGATGAGAAATACTTTCGGAAACCCTACAACTTTCTGAAGTACCACTTTGAAGCCCTG
GCAGATACTGGAATCTCCTCAGAGTTTTATGATAATGCAAATGACCTTCTTTCCAAAGTT
AAAAAAGACAAGTCTGACTCATTTGGAGTGACCATCGGCATAGGCCCAGCCGGCAGCCCT
TTATTGGTGGGTGTAGGTGTATCCCACTCACAAGACACTTCATTCTTGAACGAATTAAAC
AAGTATAATGAGAAGAAATTCATTTTCACAAGAATCTTCACAAAGGTGCAGACTGCACAT
TTTAAGATGAGGAAGGATGACATTATGCTGGATGAAGGAATGCTGCAGTCATTAATGGAG
CTTCCAGATCAGTACAATTATGGCATGTATGCCAAGTTCATCAATGACTATGGCACCCAT
TACATCACATCTGGATCCATGGGTGGCATTTATGAATATATCCTGGTGATTGACAAAGCA
AAAATGGAATCCCTTGGTATTACCAGCAGAGATATCACGACATGTTTTGGAGGCTCCTTG
GGCATTCAATATGAAGACAAAATAAATGTTGGTGGAGGTTTATCAGGAGACCATTGTAAA
AAATTTGGAGGTGGCAAAACTGAAAGGGCCAGGAAGGCCATGGCTGTGGAAGACATTATT
TCTCGGGTGCGAGGTGGCAGTTCTGGCTGGAGCGGTGGCTTGGCACAGAACAGGAGCACC
ATTACATACCGTTCCTGGGGGAGGTCATTAAAGTATAATCCTGTTGTTATCGATTTTGAG
ATGCAGCCTATCCACGAGGTGCTGCGGCACACAAGCCTGGGGCCTCTGGAGGCCAAGCGC
CAGAACCTGCGCCGCGCCTTGGACCAGTATCTGATGGAATTCAATGCCTGCCGATGTGGG
CCTTGCTTCAACAATGGGGTGCCCATCCTCGAGGGCACCAGCTGCAGGTGCCAGTGCCGC
CTGGGTAGCTTGGGTGCTGCCTGTGAGCAAACACAGACAGAAGGAGCCAAAGCAGATGGG
AGCTGGAGTTGCTGGAGCTCCTGGTCTGTATGCAGAGCAGGCATCCAGGAAAGGAGAAGA
GAGTGTGACAATCCAGCACCTCAGAATGGAGGGGCCTCGTGTCCAGGGCGGAAAGTACAG
ACGCAGGCTTGCTGA

Clone variation with respect to NM_000562.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_000562 unedited
TCGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGTACAGGTCC
CAGCCTGTAGACATCTTTTACTCCAATTTCCTGAATAGATAGCTTTATTCCTTCAAGGTA
ATATAGTGCGGTGGCTTCTGGCTGAGATGTTTGCTGTTGTTTTCTTCATCTTGTCTTTGA
TGACTTGTCAGCCTGGGGTAACTGCACAGGAGAAGGTGAACCAGAGAGTAAGACGGGCAG
CTACACCCGCAGCAGTTACCTGCCAGCTGAGCAACTGGTCAGAGTGGACAGATTGCTTTC
CGTGCCAGGACAAAAAGTACCGACACCGGAGCCTCTTGCAGCCAAACAAGTTTGGGGGAA
CCATCTGCAGTGGTGACATCTGGGATCAAGCCAGCTGCTCCAGTTCTACAACTTGTGTAA
GGCAAGCACAGTGTGGACAGGATTTCCAGTGTAAGGAGACAGGTCGCTGCCTGAAACGCC
ACCTTGTGTGTAATGGAGACCAGGACTGCCTTGATGGCTCTGATGAGGACGACTGTGAAG
ATGTCAGGGCCATTGACGAAGACTGCAGCCAGTATGAACCAATTCCAGGATCACAGAAGG
CAGCCTTGGGGTACAATATCCTGACCCAGGAAGATGCTCAGAGTGTGTACGATGCCAGTT
ATTATGGGGGCCAGTGTGAGACGGTATACAATGGGGAATGGAGGGAGCTTCGATATGACT
CCACCTGTGAACGTCTCTACTATGGAGATGATGAGAAATACTTTCNGAAACCCCTACACT
NTCTGAAGTACCANCTTTGAAGCCCTGNCAGATACTGGGATCTCCTCAGAGTTTTATGAN
TATGCAAATGACCTTCTTTTCCAAGNNTAAAAAGACAAGTCTGACTCATTTGNAGTGACC
ATNCGNCATAGCCCAGCCGCAGCCCTTTTATGGTGGNTTGTAGTGTATCCACTC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_000562 unedited
ATCTTGNACCGCGCCGCAATCTANGATNCGGTTTTTTTTTTTTTTTTTTCATCCTACTTT
GTTTTATTGGGCGTTGATTGTTTCAGGTGTCTGCTTGTTTTGTCTCTAACATCCTGTAAG
TTCTCCCTGGTCATGGATTGAGTTTTCTCAGTTTTCTATTTTACTTTATTTTTTTGATGA
TCCAAAGTGCTGTAAGTAGGACAGAGCTTCTAGTTAACAGTCAACATCCAGTTGCTGAAT
AAAATGATTGAGTTTTGTAACTAATAGAATAGTTGGCTGTAGACAGCAAAACCTAGTCTT
AGGCCTGGAAGCTGGCAAAACAAAGAAAAAAGTGTGCTGATTTGCATCTTCTCTTAGTTG
AAAGAAGTCTTTATCCAATAGTCAGTGCAGGGGTCGACATCCACAGCATCTGGTCCAGCC
TGTGTCCAGAGGCCCTCAGCAAGCCTGCGTCTGTACTTTCCGCCCTGGACACGAGGCCCC
TCCATTCTGAGGTGCTGGATTGTCACACTCTCTTCTCCTTTCCTGGATGCCTGCTCTGCA
TACAGACCAGGAGCTCCAGCAACTCCAGCTCCCATCTGCTTTGGCTCCTTCTGTCTGTGT
TTGCTCACAGGCAGCACCCAAGCTACCCAGGCGGCACTGGCACCTGCAGCTGGTGCCCTC
GAGGATGGGCACCCCATTGTTGAAGCAAGGCCCACATCGGCAGGCATTGAATTCCATCAG
ATACTGGTCCAGGCGCGGCGCAGGTTCTGGCGCTTGGCCTCCANAGGCCCCAGGCTTGTG
TGCCCGCACACCTCGTGGATAGGCTGCATCTCAAAATCGATAACACCAGGATTATACTTT
AATGACCTNCCCCAGNAACNGTATGTAATGGNTGCTCCTGNNTCTGTGCCAAGCCACCGT
TCCAGCCAGAACTGNCACCTNGCACCCGAGAAANTATGTCTTCACANCATGGCCTTNCTG
CCNTNTNAGTTTTGCCACTCCAANTTTTACN
Restriction Sites NotI-NotI |
ACCN NM_000562
Insert Size 2300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000562.1, NP_000553.1
RefSeq Size 2397 bp
RefSeq ORF 1755 bp
Locus ID 731
UniProt ID P07357
Cytogenetics 1p32.2
Domains ldl_recept_a, MACPF, tsp_1
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
Summary C8 is a component of the complement system and contains three polypeptides, alpha, beta and gamma. This gene encodes the alpha subunit of C8. C8 participates in the formation of the membrane attack complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause complement C8 alpha-gamma deficiency. provided by RefSeq, Nov 2008
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Documents
Resources
"NM_000562" in other vectors (4)
SKU Description Size Price
RC221824 C8A (Myc-DDK-tagged)-Human complement component 8, alpha polypeptide (C8A) 10 ug
$544.00
RC221824L3 Lenti ORF clone of Human complement component 8, alpha polypeptide (C8A), Myc-DDK-tagged 10 ug
$844.00
RC221824L4 Lenti ORF clone of Human complement component 8, alpha polypeptide (C8A), mGFP tagged 10 ug
$844.00
RG221824 C8A (tGFP-tagged) - Human complement component 8, alpha polypeptide (C8A) 10 ug
$744.00

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