CSTF3 (NM_001326) Human Untagged Clone

SKU SC119307

CSTF3 (untagged)-Human cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa (CSTF3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CSTF3
• Synonyms: CSTF-77
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119307 sequence for NM_001326 edited (data generated by NextGen Sequencing); ATGTCAGGAGACGGAGCCACGGAGCAGGCAGCTGAGTATGTCCCAGAGAAGGTGAAGAAA; GCGGAAAAGAAATTAGAAGAGAATCCATATGACCTTGATGCTTGGAGCATTCTCATTCGA; GAGGCACAGAATCAACCTATAGACAAAGCACGGAAGACTTATGAACGCCTTGTTGCCCAG; TTCCCCAGTTCTGGCAGATTCTGGAAACTGTACATTGAAGCAGAGATTAAAGCTAAAAAT; TATGACAAGGTTGAAAAGCTATTTCAGAGATGCCTTATGAAGGTTTTGCACATTGATTTA; TGGAAGTGTTATCTTTCATATGTCCGAGAAACCAAGGGTAAACTACCAAGTTACAAAGAA; AAAATGGCTCAAGCATATGACTTTGCACTGGATAAAATTGGAATGGAAATTATGTCCTAT; CAGATTTGGGTGGATTACATCAATTTCCTAAAAGGCGTGGAAGCTGTAGGATCTTATGCA; GAAAATCAAAGAATAACAGCTGTCCGAAGAGTTTATCAACGAGGTTGTGTTAATCCGATG; ATCAACATTGAACAGCTCTGGAGAGACTATAACAAGTATGAAGAGGGTATCAATATTCAT; TTAGCTAAAAAAATGATTGAAGATCGGAGTAGAGATTATATGAATGCTAGACGTGTAGCA; AAGGAATATGAGACAGTAATGAAAGGCTTGGACCGTAATGCTCCCTCGGTGCCTCCTCAG; AATACTCCTCAAGAAGCTCAACAAGTAGATATGTGGAAGAAATATATACAGTGGGAAAAG; AGCAACCCTCTTCGTACAGAGGATCAGACCCTTATAACAAAAAGAGTTATGTTTGCTTAT; GAACAGTGCCTGCTTGTGCTGGGCCATCACCCTGATATTTGGTATGAAGCTGCCCAGTAT; CTTGAGCAGTCAAGTAAACTGCTCGCAGAAAAGGGAGATATGAATAATGCCAAATTATTT; AGTGATGAAGCTGCTAATATATATGAAAGAGCCATAAGCACTTTATTGAAGAAGAATATG; CTTTTTTATTTTGCATATGCAGATTATGAAGAGAGTCGCATGAAGTATGAAAAGGTTCAC; AGTATATATAACAGACTTCTGGCAATTGAGGATATTGACCCTACCTTGGTATATATCCAA; TATATGAAATTTGCACGGAGAGCAGAAGGCATCAAATCTGGAAGAATGATATTTAAAAAA; GCAAGAGAAGATACCAGAACCCGCCACCATGTCTATGTTACTGCAGCACTCATGGAATAT; TACTGTAGTAAGGACAAATCTGTTGCCTTTAAGATTTTTGAGCTGGGGCTAAAAAAATAT; GGAGACATTCCAGAGTATGTCCTGGCCTATATTGACTATCTTTCTCACCTCAATGAGGAC; AATAATACCCGAGTTTTGTTTGAACGAGTTTTAACATCTGGAAGCCTTCCTCCTGAGAAG; TCTGGAGAAATCTGGGCCCGATTTCTAGCATTTGAAAGTAATATTGGTGATCTAGCTAGT; ATACTCAAAGTGGAGAAAAGACGGTTTACAGCATTCAAAGAAGAGTATGAAGGGAAAGAA; ACGGCTTTACTAGTAGATAGATACAAGTTCATGGATTTATATCCTTGCTCTGCAAGTGAA; TTAAAAGCACTTGGTTATAAGGATGTCTCCCGTGCTAAGCTAGCAGCTATAATTCCGGAC; CCAGTTGTAGCTCCTTCTATAGTGCCTGTTCTGAAAGATGAAGTGGATAGAAAACCAGAA; TACCCTAAACCAGACACTCAGCAGATGATTCCATTTCAGCCACGACATTTAGCACCTCCA; GGTTTACACCCTGTACCTGGTGGAGTGTTCCCAGTCCCTCCTGCAGCTGTTGTTTTAATG; AAACTTCTCCCTCCTCCTATCTGTTTCCAGGGTCCTTTTGTACAAGTGGATGAACTGATG; GAAATTTTCCGAAGATGCAAGATACCAAATACTGTTGAGGAAGCTGTGAGGATCATTACT; GGTGGGGCCCCAGAGCTAGCTGTAGAAGGCAACGGCCCCGTGGAAAGTAATGCAGTACTC; ACCAAGGCCGTCAAAAGGCCCAACGAGGATTCAGATGAAGATGAAGAAAAGGGAGCCGTT; GTCCCCCCTGTTCATGACATTTACAGAGCACGGCAGCAGAAGCGGATTCGGTAG; ; Clone variation with respect to NM_001326.2; 1024 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001326 unedited; NGTCGGATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGCGATTGGTG; TTGGCGGTCTGGCTCAGCTGGGCAGGGGGTAACTTTACTGATTTGGGGGTGGTTTTTAGT; TTAATTTTTCTTTTCTAGCTTCCCATCGACGGTCAGTGCGCACGTTGTAATCAGCTGAGG; CCATGTCAGGAGACGGAGCCACGGAGCAGGCAGCTGAGTATGTCCCAGAGAAGGTGAAGA; AAGCGGAAAAGAAATTAGAAGAGAATCCATATGACCTTGATGCTTGGAGCATTCTCATTC; GAGAGGCACAGAATCAACCTATAGACAAAGCACGGAAGACTTATGAACGCCTTGTTGCCC; AGTTCCCCAGTTCTGGCAGATTCTGGAAACTGTACATTGAAGCAGAGATTAAAGCTAAAA; ATTATGACAAGGTTGAAAAGCTATTTCAGAGATGCCTTATGAAGGTTTTGCACATTGATT; TATGGAAGTGTTATCTTTCATATGTCCGAGAAACCAAGGGTAAACTACCAAGTTACAAAG; AAAAAATGGCTCAAGCATATGACTTTGCACTGGATAAAATTGGAATGGAAATTATGTCCT; ATCAGATTTGGGTGGATTACATCAATTTCCTAAAAGGCGTGGAAGCTGTAGGATCTTATG; CAGAAAATCAAAGAATAACAGCTGTCCGAAGAGTTTATCAACGAGGTTGTGTTAATCCGA; TGATCAACATTGAACAGCTCTGGAGAGACTATAACAAGTATGAAGAGGGTATCAATATTC; ATTTAGCTAAAAAATGATTGAAGATCGCAGTAGAGATTATATGAATGCTAGACGTGTAGC; ANAGGGATATGAGACAGNNTATGAAAGGCTGGNACCCGTAATGCTCCTCCGTGCCTCCTC; AGAATACTCCTCAGAGCTCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001326 unedited; GTGGATCCTTTTTTTTTCTAAAGGACTTTCTAAAATTATGAAACTACAAAACGGATTTCA; TACATTCTGCCATAAATAAAAACAAACAATAAGGCAGGACTCTACATAAGCAAACCAAGA; ACAATTGTTTTCAGAAAATGTGATAAAATGCTTTACAATCATAAGCCATCCAGTTTTTAA; AATAAAACTGTTGAAAAACTCACAAATCTTCAAGCGGCACATACAAGACACCCTTGAAGA; GGGAACAAAATGTGGTTCTGCCACTTTGTACTGTTCTCACATTTTTGAAAACCTAATTTT; GCTTATAGCATAGGTCTGTTCCGTATTCTAAAATTCACACAGGTTGGTTTGTTTTTTCTC; AAGAACCATGTGATAGAGGCACCAATGACAATACAACTTTGTTTCCAAGAACCTTGTAAC; AAAGCGTTGTCTCTTTTAAACATACCACTTGAGGCAAAAGGAATCCTGGACAGGAGTTTT; CTGTAGAGGCGTTTAAAACCCTACCGAATCCGCTTCTGCTGCCGTGCTCTGTAAATGTCA; TGAACAGGGGGGACAACGGCTCCCTTTTCTTCATCTTCATCTGAATCCTCGTTGGGCCTT; TTGACGGCCTTGGTGAGTACTGCATTACTTTCCACGGGGCCGTTGCCTTCTACAGCTAGC; TCTGGGGCCCCACCAGTAATGATCCTCACAGCTTCCTCAACAGTATTTGGTATCTTGCAT; CTTCGGAAAATTTCCATCAGTTCATCCACTTGTACAAAAAGACCCTGNAAACAGATAGGA; TGATGGAGATAGTTCATTAAACACAGCTGCNAGAGGACTGGGACACTCCACCAGTACATG; GTGTAACCTGGAGTGCTAATGTCGGGCTGAATGGATCTCTGCTGATGTCTGTTTAAGGTA; TCTAGTTTTCATCCACTCATCTTTANAACAGCCTATAAATGAGCTCACTGGGTCCGATAT; AGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001326
• Insert Size: 3250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001326.1, NP_001317.1
• RefSeq Size: 2766 bp
• RefSeq ORF: 2154 bp
• Locus ID: 1479
• UniProt ID: Q12996
• Cytogenetics: 11p13
• Domains: HAT
• Summary: The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).