cleavage stimulation factor (CSTF1) (NM_001324) Human Untagged Clone

SKU SC119305

CSTF1 (untagged)-Human cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa (CSTF1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: cleavage stimulation factor
• Synonyms: CstF-50; CstFp50
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119305 sequence for NM_001324 edited (data generated by NextGen Sequencing); ATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGATCATTAGC; CAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAATCAAGCCT; CAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAATGGAAAAC; GATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTGGCACAGGG; ATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTGAGTACGAA; ACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTAGAGATGGA; CAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAGAGAGGATG; TTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAACAAAATATG; GAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGTGCCTTGCT; TTCCACCCAACAGAACAGATCCTGGCTTCTGGTTCAAGGGATTATACTCTTAAATTATTT; GATTATTCCAAACCATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGAAATGTTA; CGTTCCATCTCTTTTCATCCTTCTGGAGACTTTATACTTGTCGGAACTCAGCATCCTACT; CTTCGCCTTTATGATATCAACACCTTTCAATGTTTTGTCTCTTGCAATCCTCAAGATCAA; CACACCGATGCTATATGTTCCGTTAATTACAATTCTAGTGCCAATATGTACGTAACTGGA; AGCAAGGACGGCTGCATCAAATTATGGGATGGTGTTTCAAATCGATGCATCACAACTTTT; GAGAAAGCACATGACGGTGCTGAAGTTTGTTCTGCCATTTTTTCCAAAAATTCTAAATAC; ATTCTCTCAAGTGGAAAAGACTCTGTAGCTAAACTTTGGGAAATATCAACGGGACGAACA; CTGGTCAGATACACGGGCGCGGGTTTAAGTGGACGCCAGGTGCACCGGACACAGGCTGTG; TTTAACCACACCGAGGACTATGTGTTGCTGCCCGACGAGAGGACGATCAGTCTTTGCTGC; TGGGACTCGAGGACAGCCGAGCGGAGAAACCTGCTGTCGTTGGGGCACAACAATATTGTA; CGCTGCATAGTGCACTCCCCCACCAACCCCGGGTTCATGACGTGCAGCGATGACTTCAGA; GCGCGGTTTTGGTACCGGAGATCGACCACTGACTGA; ; Clone variation with respect to NM_001324.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001324 unedited; TGTTCACATTTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGTTTTTCCA; GGAGAGAGCGGGATACCAAGAGAACCGGACCAGCTGCTGGCAGGGAAACTGTCTTCCTTT; TCTCCAAGATGTACAGAACCAAAGTGGGCTTGAAGGACCGCCAGCAGCTCTACAAGCTGA; TCATTAGCCAGCTGCTATATGACGGCTACATCAGCATCGCCAATGGCCTCATCAATGAAA; TCAAGCCTCAGTCTGTGTGTGCACCCTCGGAGCAGCTCCTGCATCTCATCAAACTCGGAA; TGGAAAACGATGACACCGCAGTTCAGTATGCAATTGGTCGTTCAGATACTGTTGCCCCTG; GCACAGGGATTGACCTGGAATTTGATGCAGATGTTCAGACTATGTCCCCAGAGGCTTCTG; AGTACGAAACATGCTATGTCACATCACATAAAGGACCATGCCGTGTAGCTACCTATAGTA; GAGATGGACAGTTAATAGCTACTGGGTCTGCTGATGCTTCGATAAAGATACTTGACACAG; AGAGGATGTTGGCCAAAAGTGCCATGCCAATAGAGGTCATGATGAATGAGACCGCACAAC; AAAATATGGAAAACCACCCAGTGATTCGAACTCTTTATGACCATGTGGATGAAGTCACGT; GCCTTGCTTTCCACCCAACAGAACAGATCCTGGCTTCTGGGTCAAGGGATTATACTCTTA; AAATATNTGATTATTCCAAACATCAGCAAAAAGAGCCTTCAAATACATTCAGGAAGCTGA; AATGTTACGGTCCATCTCTTTTCATCCCTCTGGAGACTTTATACTTGGTGGGACTCAGCA; TCCTACTCTTCGCCTTTAGAAATCAACACCCTTTAAAGTTTTGTCCTCTTGCAATCTCCA; GATCAACACAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001324
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001324.1, NP_001315.1
• RefSeq Size: 1801 bp
• RefSeq ORF: 1296 bp
• Locus ID: 1477
• UniProt ID: Q05048
• Cytogenetics: 20q13.2-q13.31
• Domains: WD40
• Summary: This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.