ECH1 (NM_001398) Human Untagged Clone
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | ECH1 |
| Synonyms | HPXEL |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_001398 |
| Insert Size | 1290 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001398.2, NP_001389.2 |
| RefSeq Size | 1276 bp |
| RefSeq ORF | 987 bp |
| Locus ID | 1891 |
| UniProt ID | Q13011 |
| Cytogenetics | 19q13.2 |
| Domains | ECH |
| Summary | This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001398" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205622 | ECH1 (Myc-DDK-tagged)-Human enoyl CoA hydratase 1, peroxisomal (ECH1) | 10 ug |
$300.00
|
|
| RC205622L3 | Lenti ORF clone of Human enoyl CoA hydratase 1, peroxisomal (ECH1), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC205622L4 | Lenti ORF clone of Human enoyl CoA hydratase 1, peroxisomal (ECH1), mGFP tagged | 10 ug |
$600.00
|
|
| RG205622 | ECH1 (tGFP-tagged) - Human enoyl CoA hydratase 1, peroxisomal (ECH1) | 10 ug |
$489.00
$500.00
|
|
Citations
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