PRMT2 (NM_001535) Human Untagged Clone

SKU SC119192

PRMT2 (untagged)-Human protein arginine methyltransferase 2 (PRMT2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: PRMT2
• Synonyms: HRMT1L1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119192 sequence for NM_001535 edited (data generated by NextGen Sequencing); ATGGCAACATCAGGTGACTGTCCCAGAAGTGAATCGCAGGGAGAAGAGCCTGCTGAGTGC; AGTGAGGCCGGTCTCCTGCAGGAGGGAGTACAGCCAGAGGAGTTTGTGGCCATCGCGGAC; TACGCTGCCACCGATGAGACCCAGCTCAGTTTTTTGAGAGGAGAAAAAATTCTTATCCTG; AGACAAACCACTGCAGATTGGTGGTGGGGTGAGCGTGCGGGCTGCTGTGGGTACATTCCG; GCAAACCATGTGGGGAAGCACGTGGATGAGTACGACCCCGAGGACACGTGGCAGGATGAA; GAGTACTTCGGCAGCTATGGAACTCTGAAACTCCACTTGGAGATGTTGGCAGACCAGCCA; CGAACAACTAAATACCACAGTGTCATCCTGCAGAATAAAGAATCCCTGACGGATAAAGTC; ATCCTGGACGTGGGCTGTGGGACTGGGATCATCAGTCTCTTCTGTGCACACTATGCGCGG; CCTAGAGCGGTGTACGCGGTGGAGGCCAGTGAGATGGCACAGCACACGGGGCAGCTGGTC; CTGCAGAACGGCTTTGCTGACATCATCACCGTGTACCAGCAGAAGGTGGAGGATGTGGTG; CTGCCCGAGAAGGTGGACGTGCTGGTGTCTGAGTGGATGGGGACCTGCCTGCTGTTTGAG; TTCATGATCGAGTCCATCCTGTATGCCCGGGATGCCTGGCTGAAGGAGGACGGGGTCATT; TGGCCCACCATGGCTGCGTTGCACCTTGTGCCCTGCAGTGCTGATAAGGATTATCGTAGC; AAGGTGCTCTTCTGGGACAACGCGTACGAGTTCAACCTCAGCGCTCTGAAATCTTTAGCA; GTTAAGGAGTTTTTTTCAAAGCCCAAGTATAACCACATTTTGAAACCAGAAGACTGTCTC; TCTGAACCGTGCACTATATTGCAGTTGGACATGAGAACCGTGCAAATTTCTGATCTAGAG; ACCCTGAGGGGCGAGCTGCGCTTCGACATCAGGAAGGCGGGGACCCTGCACGGCTTCACG; GCCTGGTTTAGCGTCCACTTCCAGAGCCTGCAGGAGGGGCAGCCGCCGCAGGTGCTCAGC; ACCGGGCCCTTCCACCCCACCACACACTGGAAGCAGACGCTGTTCATGATGGACGACCCA; GTCCCTGTCCATACAGGAGACGTGGTCACGGGTTCAGTTGTGTTGCAGAGAAACCCAGTG; TGGAGAAGGCACATGTCTGTGGCTCTGAGCTGGGCTGTCACTTCCAGACAAGACCCCACA; TCTCAAAAAGTTGGAGAAAAAGTCTTCCCCATCTGGAGATGA; ; Clone variation with respect to NM_001535.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001535 unedited; GTGGATTTTGAAACCGACTTCCTATAGGGCGGCCGCGAATTCGGCACGAGGGGCTCCTGT; AAAGGACCGTCCACCCCTCCGCGCTGGCGGTGTGGACGCGGAACTCAGCGGAGAAACGCG; ATTGAGAAATGGAAAAGAAAATGAAATAAATCAGCAGTTATGAGGCAGAGCCTAAGAGAA; CTATGGCAACATCAGGTGACTGTCCCAGAATTGTTTCGCAGGGAGAAGAGCCTGCTGAGT; GCAGTGAGGCCGGTCTCCTGCAGGAGGGAGTACAGCCAGAGGAGTTTGTGGCCATCGCGG; ACTACGCTGCCACCGATGAGACCCAGCTCAGTTTTTTGAGAGGAGAAAAAATTCTTATCC; TGAGACAAACCACTGCAGATTGGTGGTGGGGTGAGCGTGCGGGCTGCTGTGGGTACATTC; CGGCAAACCATGTGGGGAAGCACGTGGATGAGTACGACCCCGAGGACACGTGGCAGGATG; AAGAGTACTTCGGCAGCTATGGAACTCTGAAACTCCACTTGGAGATGTTGGCAGACCAGC; CACGAACAACTAAATACCACAGTGTCATCCTGCAGAATAAAGAATCCCTGACGGATAAAG; TCATCCTGGACGTGGGCTGTGGGACTGGGATCATCAGTCTCTTCTGTGCACACTATGCGC; GGCCTAGAGCGGTGTACGCGGTGGAGGCCAGTGAGATGGCACAGCACACGGNGCAGCTGG; TCCTGCAGAACGGCTNTGCTGACATCATCACCGTGTACCAGCANAAGGTGGAGGATGTGG; TGCTGCCCGAGAAGTGGACGTGCNTGTGTCTGAGTGGATGGNGACCTGCCCTGCTGTTGA; GTTCATGATCGAGNTCATCCTGTATGCCCCGNATGCCTGGCTGANNGAGGACCGGGTCAT; TTGCCCACCATGGCTGCGNTGCACCTTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001535 unedited; ATGGAAACTTCCATTTTCCAGNANAGCACTGGGCGAGGGGTCACAGGGCTGCCACCCGGG; ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT; TTTCTCACTCATTTAAAAATATTTATTGAGCATGTGCGATGGACCAACACATAATTTATT; TGGAAGACGTCTACACAACTGTACTATCCCATGCATAGTCCCATCGCATACATCACCACC; TTAGCTCGTACGCTGAGCCAGGGCAGTGTTCCCTACACGCCACTGGCACAGCCCTGTGCA; ATCCTACGTCCCACACGAGTGGATGAGCGACGGCAGTAAGGGCACCACTAACATAGGAGG; TGGGGACAGTCACTGTCACAGGGTACACGGGCTGATATGCGTGCGTCCTAGGAGTGTAGA; CCTAGACCTAGCTAAGGAACATGGAGCTCCGACCCAGCCACGGGTCGGCACTGTGGGCAC; CACGGGTGAACACCTGAAAACACAGCCTACTTCTAGAGGGCAAACACTGGGCAGCGGGGG; TGGCCAACGGAAGTTTCTGCCGAGCCCAACGTGTTTGTTTGGGAAGGACCCTGCCTCCCC; TGCCAGGCTGGGGAGGGGTAAATGGGGAGTGTAAGTTCCACCGACTTCGCGAGAGTGTGC; AAAAGCCACGTGCACTTGGTTCGCTTGTTTCATTCCCCCCCAAGATTGCCCCCTGCTTTC; CAAAAAGCTTCCCCTGCATTCCCCAAAGGGGAGACATTTCCTCCACTTTTGAAACGGGGG; TTTTGCCGGGAGTGAAACCCAATTTAAAACCCAAAACGGGCCTTCCCAACTGGGTTTCTC; GGAAAACATCGAACCCTTGACCCCTCTTTGTATGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001535
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001535.2, NP_001526.2
• RefSeq Size: 2140 bp
• RefSeq ORF: 1302 bp
• Locus ID: 3275
• UniProt ID: P55345
• Cytogenetics: 21q22.3
• Domains: SH3
• Protein Families: Druggable Genome
• Summary: Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1).