PARP1 (NM_001618) Human Untagged Clone
$1,325.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | PARP1 |
| Synonyms | ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_001618 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | TrueClone. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001618.2, NP_001609.1 |
| RefSeq Size | 3859 bp |
| RefSeq ORF | 3045 bp |
| Locus ID | 142 |
| UniProt ID | P09874 |
| Cytogenetics | 1q42.12 |
| Domains | BRCT, PARP, PARP_reg, zf-PARP |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transcription Factors |
| Protein Pathways | Base excision repair |
| Summary | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001618" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207085 | PARP1 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase 1 (PARP1) | 10 ug |
$1,324.00
|
|
| RC207085L1 | Lenti ORF clone of Human poly (ADP-ribose) polymerase 1 (PARP1), Myc-DDK-tagged | 10 ug |
$1,624.00
|
|
| RC207085L2 | Lenti ORF clone of Human poly (ADP-ribose) polymerase 1 (PARP1), mGFP tagged | 10 ug |
$1,624.00
|
|
| RC207085L3 | Lenti ORF clone of Human poly (ADP-ribose) polymerase 1 (PARP1), Myc-DDK-tagged | 10 ug |
$1,624.00
|
|
| RC207085L4 | Lenti ORF clone of Human poly (ADP-ribose) polymerase 1 (PARP1), mGFP tagged | 10 ug |
$1,624.00
|
|
| RG207085 | PARP1 (tGFP-tagged) - Human poly (ADP-ribose) polymerase 1 (PARP1) | 10 ug |
$1,524.00
|
|
Citations
2,3,7,8-Tetrachlorodibenzo-p-dioxin poly(ADP-ribose) polymerase (TiPARP, ARTD14) is a mono-ADP-ribosyltransferase and repressor of aryl hydrocarbon receptor transactivation
MacPherson, Tamblyn, Rajendra et al
Nucleic Acids Res. (2013) 41 (3), 1604-21 DOI: 10.1093/nar/gks1337
MacPherson, Tamblyn, Rajendra et al
Nucleic Acids Res. (2013) 41 (3), 1604-21 DOI: 10.1093/nar/gks1337
Cited by 180
AI Summary: OriGene’s pCMV-XL-PARP-1 and pCMV-XL-PARP-12 expression plasmids were used in this study to investigate the catalytic functions of PARP family members, helping to elucidate TiPARP’s role as a mono-ADP-ribosyltransferase and repressor of aryl hydrocarbon receptor transactivation.
Snippet: Gardner) or 200 ng pcDNA-mTiparp, pcDNA-chTiPARP, 200–1500 ng pCMV-XL-PARP-1 or pCMV-XL-PARP-12 (Origene) or 200 ng ... Membranes were incubated with anti-AHR (Enzo Life Sciences Inc., SA-210) overnight and then incubated for 1 h with anti-rabbit horseradish
BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8
Yan, Xu, Zhu et al
Mol. Cell. Biol. (2013) 33 (4), 845-57 DOI: 10.1128/MCB.00990-12
Yan, Xu, Zhu et al
Mol. Cell. Biol. (2013) 33 (4), 845-57 DOI: 10.1128/MCB.00990-12
Cited by 1
AI Summary: OriGene’s BAL1 antibody (SC119157) was used in this study to detect BAL1 protein, facilitating the investigation of its role in DNA damage response and repair mechanisms.
Snippet: SC119157) was purchased from OriGene Technologies (Rockville, MD). ... Billerica, MA), BBAP (mouse monoclonal antibody [14] or rabbit polyclonal antibody, A300-833A [Bethyl Laboratories]), FK2 (multiubiquitin monoclonal antibody, SPA-205;Enzo Life Sciences International
SIRT6 overexpression induces massive apoptosis in cancer cells but not in normal cells
Van Meter, Mao, Gorbunova et al
Cell Cycle (2011) 10 (18), 3153-8
Van Meter, Mao, Gorbunova et al
Cell Cycle (2011) 10 (18), 3153-8
Cited by 26
Cooperative role of NF-{kappa}B and poly(ADP-ribose) polymerase 1 (PARP-1) in the TNF-induced inhibition of PHEX expression in osteoblasts
Majewski, Thurston, Ramalingam et al
J Biol Chem (2010) 285 (45), 34828-38 DOI: 10.1074/jbc.M110.152868
Majewski, Thurston, Ramalingam et al
J Biol Chem (2010) 285 (45), 34828-38 DOI: 10.1074/jbc.M110.152868
AI Summary: In this study, OriGene products including the RelA (NM_009045) cDNA clone and PARP-1 (NM_001618) cDNA clone were used for overexpression experiments to investigate the cooperative roles of NF-κB and PARP-1 in the TNF-induced inhibition of PHEX expression in osteoblasts.
Poly(ADP-ribose) polymerase-1 regulates vimentin expression in lung cancer cells
Chu, Xu, Ferro et al
Am J Physiol Lung Cell Mol Physiol (2007) 293 (5), L1127-34 DOI: 10.1152/ajplung.00197.2007
Chu, Xu, Ferro et al
Am J Physiol Lung Cell Mol Physiol (2007) 293 (5), L1127-34 DOI: 10.1152/ajplung.00197.2007
Cited by 23
AI Summary: In this study, OriGene products—specifically the human vimentin cDNA clone (SC119499) and anti-vimentin antibody (TA309184)—were used to analyze vimentin expression and regulation in lung cancer cells, enabling the authors to investigate how poly(ADP-ribose) polymerase-1 (PARP-1) influences vimentin promoter activity and protein levels.
2,3,7,8-TETRACHLORODIBENZO-p-DIOXIN-INDUCIBLE POLY(ADP-RIBOSE) POLYMERASE IS A MONO-ADPRIBOSYLTRANSFERASE AND A LIGAND-INDUCED REPRESSOR OF AHR TRANSACTIVATION
MacPherson
Thesis (2014)
MacPherson
Thesis (2014)
Snippet: All full-length human TiPARP expression constructs, TiPARP N-terminal truncation expression constructs and the C-terminal truncation 1-234 were created by PCR amplification of TiPARP cDNA from pCMV6-XL4-hTiPARP (Origene, Rockwood, MD). Two-hundred micrograms of pCMV6-XL4-hTiPARP was added to a reaction mixture containing 0.2 μM of desired forward primer containing Kozak sequence and/or EcoRI r...
Showing 1-6 of 6 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
