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BLK (NM_001715) Human Untagged Clone

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SKU
SC119056
BLK (untagged)-Human B lymphoid tyrosine kinase (BLK)
  $471.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol BLK
Synonyms MODY11
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_001715 edited
GTTGGAAGTTGCTCGTTGTCGCTAGGAGCCTGCTCCACTGTAAGGGTGTCGGGATCTGAA
GAGCTATGGTGAAACACCACTGAAGCATTGCCAAGGATGGGGCTGGTAAGTAGCAAAAAG
CCGGACAAGGAAAAGCCGATCAAAGAGAAGGACAAGGGCCAATGGAGCCCCCTGAAGGTC
AGCGCCCAAGACAAGGACGCCCCGCCACTGCCGCCCCTGGTTGTCTTCAACCACCTTACT
CCTCCACCGCCCGATGAACACCTGGATGAAGACAAGCATTTCGTGGTGGCTCTGTATGAC
TACACCGCTATGAATGATCGGGACCTGCAGATGCTGAAGGGGGAGAAGCTACAGGTCCTG
AAGGGAACTGGAGACTGGTGGCTGGCCAGGTCACTCGTCACAGGAAGAGAAGGCTATGTG
CCCAGTAACTTTGTGGCCCGAGTGGAGAGCCTGGAAATGGAAAGGTGGTTCTTTAGATCA
CAGGGTCGGAAGGAGGCTGAGAGGCAGCTTCTTGCTCCAATCAACAAGGCCGGCTCCTTT
CTTATCAGAGAGAGTGAAACCAACAAAGGTGCCTTCTCCCTGTCTGTGAAGGATGTCACC
ACCCAGGGGGAGCTGATCAAGCACTATAAGATCCGCTGCCTGGATGAAGGGGGCTACTAC
ATCTCCCCCCGGATCACCTTCCCCTCGCTCCAGGCCCTGGTGCAGCACTATTCTAAGAAG
GGGGATGGTCTATGCCAGAGGCTGACCCTGCCCTGTGTGCGCCCGGCCCCGCAGAATCCC
TGGGCCCAGGATGAATGGGAGATCCCCCGGCAGTCTCTCAGGCTGGTCAGGAAACTCGGG
TCTGGACAATTCGGCGAAGTCTGGATGGGTTACTACAAAAACAACATGAAGGTGGCCATT
AAGACGCTGAAGGAGGGAACCATGTCTCCAGAAGCCTTCCTGGGTGAGGCCAACGTGATG
AAGGCTCTGCAGCACGAGCGGCTGGTCCGACTCTACGCAGTGGTCACCAAGGAGCCCATC
TACATTGTCACCGAGTACATGGCCAGAGGATGCCTGCTGGATTTCCTGAAGACAGATGAA
GGGAGCAGATTGTCACTCCCAAGGCTGATTGACATGTCGGCGCAGATTGCTGAAGGGATG
GCATACATTGAGCGCATGAATTCCATCCACCGCGACCTGCGGGCGGCCAACATCCTGGTG
TCTGAGGCCTTGTGCTGCAAAATTGCTGATTTTGGCTTGGCTCGAATCATCGACAGTGAA
TACACGGCCCAAGAGGGGGCCAAGTTCCCCATCAAGTGGACAGCCCCGGAAGCCATCCAC
TTCGGGGTCTTCACCATCAAAGCAGACGTGTGGTCGTTTGGAGTCCTCCTGATGGAAGTT
GTCACTTATGGGCGGGTGCCATACCCAGGGATGAGCAACCCCGAGGTCATCCGCAACCTG
GAGCGCGGCTACCGCATGCCGCGCCCCGACACCTGCCCGCCCGAGCTGTACCGCGGCGTC
ATCGCCGAGTGCTGGCGCAGCCGGCCCGAGGAGCGGCCCACCTTCGAGTTCCTGCAGTCG
GTGCTGGAGGACTTCTACACGGCCACCGAGCGGCAGTACGAGCTGCAGCCCTAGCCGGCC
GCGCCCGCCTGCGCCCCGTGCCCACCTCTGCGCGGACGACCCCGACTTCCGTGCCATCCC
AGACGGGCCGCGAAGGCGGGGTGTCGCCTGTGCCCTTTTCTCAGACCCGGAATCCAGTGG
GCAGAGGCAGCTTCGCAGGGGGTCCCCGGACGGACTCCTTCACCGACTGTACCCCCGGGC
GAGTTACGCGGCCTCTCTGTGCCGCTTCATTTGTAGAGGGCTGTAACAGTGACCTCGCAC
GGTCATCCGGAGTACTAAGCCCCAGTAAGGTGTTCAGGACTGGTAAGCGACTGTCATCAA
GTAAGGCCCCCGTGCTGGGCACCCCCCGTGCTGGCCGCGTCCCCGCCTCTGCGCCCTGCG
TGGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001715 unedited
CAGGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAGGCTCT
GATCGCAGACCGGGGGTGCTGCTACCTCTGTCTGCTGCCGGCAGAAAGCCACAAGCCATG
AAAACTGATTGAGATGAGAAGAATTCATCTGGGACTGGCTTTTGCTTTAGGATGGTGTTG
GAAGTTGCTCGTTGTCGCTAGGAGCCTGCTCCACTGTAAGGGTGTCGGGATCTGAAGAGC
TATGGTGAAACACCACTGAAGCATTGCCAAGGATGGGGCTGGTAAGTAGCAAAAAGCCGG
ACAAGGAAAAGCCGATCAAAGAGAAGGACAAGGGCCAATGGAGCCCCCTGAAGGTCAGCG
CCCAAGACAAGGACGCCCCGCCACTGCCGCCCCTGGTTGTCTTCAACCACCTTACTCCTC
CACCGCCCGATGAACACCTGGATGAAGACAAGCATTTCGTGGTGGCTCTGTATGACTACA
CCGCTATGAATGATCGGGACCTGCAGATGCTGAAGGGGGAGAAGCTACAGGTCCTGAAGG
GAACTGGAGACTGGTGGCTGGCCAGGTCACTCGTCACAGGAAGAGAAGGCTATGTGCCCA
GTAACTTTGTGGCCCGAGTGGAGAGCCTGGAAATGGAAAGGTGGTTCTTTAGATCACAGG
GTCGGAAGGAGGCTGAGAGGCAGCTTCTTGCTCCAATCAACAAGGCCGGCTCCTTTCTTA
TCAGAGAGAGTGAANACCAACAAGGTGCCTTCTCCCTGTCTGTGAAGGATGTCACCACCC
CNGGGGGAGCTGATCAAGCACTATNAGATCCGCTGCCTGGATGAAGGGGGCTACTACATC
TCCCCCCGGATCACCTTNCCTCGCTNNCAGCCCTGGTGCAGCACTATTTCTAGAAGGGGG
ATGGTCTATGCCAGAGCTGACCTGCCCTGTGTGCGCCCGGCCCGCAAATCCCTGGCCCAG
ATGAATGGGGATCCCCGGCAGTCTTTCAGCTGGTAC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001715 unedited
NNAAAATACTTGNACCGCGCCGCATTCTAGNGACTAGTCTCGATTTTTTTTTTTTTTTTT
TTTTTTTCGAACGCTCGTGACTTTATTGCAAAAAAGCCCCGCAGTGCTGGAGCGCAGCCT
AGGCTGGGGCTGCCCCTGCTGGCGTCCGCGGGACCCAGTCTGGCTTCTGTAGCGGGGCAG
GGCGGGGTCCACGCAGGGCGCAAAGGCGGGGACGCGGCCAGCACGGGGGGTGCCCAGCAC
GGGGGCCTTACTTGATGACAGTCGCTTACCAGTCCTGAACACCTTACTGGGGCTTAGTAC
TCCGGATGACCGTGCGAGGTCACTGTTACAGCCCTCTACAAATGAAGCGGCACAGAGAGG
CCGCGTAACTCGCCCGGGGGTACAGTCGGTGAAGGAGTCCGTCCGGGGACCCCCTGCGAA
GCTGCCTCTGCCCACTGGATTCCGGGTCTGAGAAAAGGGCACAGGCGACACCCCGCCTTC
GCGGCCCGTCTGGGATGGCACGGAAGTCGGGGTCGTCCGCGCAAAGGTGGGCACGGGGCG
CAGGCGGGCGCGGCCGGCTAGGGCTGCAGCTCGTACTGCCGCTCGGTGGCCGTGTAGAAG
TCCTCCAGCACCGACTGCAGGAACTCGAAGGTGGGCCGCTCCTCGGGCCGGCTGCGCCAG
CACTCGGCGATGACGCCGCGGTACAGCTCGGGCGGGCAGGNTGTCGGGGCGCGGCATGCG
GTAGCCGCGCTCCAGGTTGCGGATGACCTCGGGGTTGCTCATCCCTGGGTATGGCACCCG
CCCATAAGTGACAACTTCCATCAGGAGGACTCCAACGACCACACGTCTGCTTTGATGGTG
AAGACCCCCGAGTGGATGGCTTCCGGGGCTGTCCACTTGATGGGGAAACTTGGCCCCCTC
TGGGGCCGTGTATTCACTGTCGATGATTCN
Restriction Sites NotI-NotI |
ACCN NM_001715
Insert Size 2500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001715.2, NP_001706.2
RefSeq Size 2642 bp
RefSeq ORF 1518 bp
Locus ID 640
UniProt ID P51451
Cytogenetics 8p23.1
Domains pkinase, SH2, SH3, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
Summary This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. provided by RefSeq, Aug 2010
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Documents
Resources
"NM_001715" in other vectors (6)
SKU Description Size Price
RC207123 BLK (Myc-DDK-tagged)-Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized 10 ug
$289.00 $470.00
RC207123L3 Lenti-ORF clone of BLK (Myc-DDK-tagged)-Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized 10 ug
$770.00
RC207123L4 Lenti-ORF clone of BLK (mGFP-tagged)-Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized 10 ug
$770.00
RG207123 BLK (tGFP-tagged) - Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized 10 ug
$670.00
SC323504 BLK (untagged)-Kinase deficient mutant (K269M) of Human B lymphoid tyrosine kinase (BLK) 10 ug
$471.00
SC323517 BLK (untagged)-Kinase deficient mutant (K269M) of Human B lymphoid tyrosine kinase (BLK) 10 ug
$471.00

file-alt Citations

Functional rare and low frequency variants in BLK and BANK1 contribute to human lupus
Jiang, Athanasopoulos, Ellyard et al
Nat Commun (2019) 10 (1), 2201 DOI: 10.1038/s41467-019-10242-9
AI Summary: OriGene products, specifically the untagged BLK and untagged IRF5 expression vectors, were used in this study to investigate the functional impact of rare and low-frequency variants in BLK and BANK1 on lupus risk.
Snippet: …Expression vectors and mutagenesis The following expression vectors were obtained: untagged BLK (OriGene Technologies), myc-LYN (GeneCopoeia, Inc.), untagged IRF5 (OriGene Technologies). …Expression vectors and mutagenesis The following expression vectors were obtained: untagged BLK (OriGene Technologies), myc-LYN (GeneCopoeia, Inc.), untagged IRF5 (OriGene Technologies). …
FigureFigureFigure
Showing 1-1 of 1 papers.
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