BLK (NM_001715) Human Tagged ORF Clone

SKU RG207123

BLK (tGFP-tagged) - Human B lymphoid tyrosine kinase (BLK). Note: ORF is codon optimized

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: BLK
• Synonyms: MODY11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207123 ORF sequence, codon optimized.; Due to the complexity of NM_001715, the ORF clone is codon optimized for mammalian Expression. ; The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.; ; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCCTGGTATCCTCTAAGAAACCCGACAAGGAAAAACCCATTAAGGAAAAGGACAAGGGACAGTGGA; GCCCCCTTAAGGTGTCAGCGCAGGATAAAGACGCACCTCCCCTGCCCCCTCTGGTTGTGTTTAACCACTT; GACCCCTCCTCCACCCGACGAGCACCTGGATGAGGACAAACACTTCGTGGTCGCCCTCTACGATTACACA; GCTATGAATGATCGCGATCTCCAGATGCTGAAGGGGGAGAAACTTCAAGTCCTCAAAGGCACTGGCGACT; GGTGGCTGGCCCGATCCCTGGTAACGGGCCGCGAGGGCTACGTTCCCAGCAATTTTGTCGCTCGCGTGGA; GTCTTTGGAGATGGAAAGGTGGTTCTTTAGATCTCAAGGCAGGAAAGAAGCTGAAAGACAGCTCCTGGCG; CCAATTAATAAAGCAGGCAGTTTCCTGATCAGAGAGAGTGAAACGAATAAGGGAGCGTTTTCTCTCTCAG; TCAAAGACGTGACTACCCAGGGAGAACTGATTAAGCACTACAAAATCCGCTGCCTTGATGAAGGTGGCTA; CTATATTTCCCCCAGAATTACTTTTCCTTCACTGCAGGCCCTGGTCCAGCACTACAGCAAGAAGGGAGAT; GGTTTGTGTCAGCGCCTGACACTCCCATGTGTTCGGCCAGCCCCCCAGAATCCATGGGCTCAAGACGAAT; GGGAAATCCCCCGGCAAAGTCTCAGACTTGTGAGGAAGCTGGGGAGTGGACAATTTGGGGAGGTCTGGAT; GGGCTACTACAAGAATAACATGAAGGTGGCAATTAAAACCCTGAAAGAAGGGACTATGAGTCCGGAGGCA; TTTCTGGGCGAGGCAAATGTGATGAAGGCCTTGCAGCACGAGCGCCTGGTAAGGTTGTATGCCGTGGTGA; CTAAGGAGCCCATCTACATCGTAACCGAATACATGGCAAGGGGGTGCCTGCTTGACTTCTTGAAAACAGA; CGAGGGCTCCAGACTGTCCCTGCCCCGGCTGATCGATATGTCAGCACAGATCGCCGAGGGCATGGCTTAC; ATCGAAAGGATGAACTCCATACACCGAGATCTTCGCGCAGCCAACATCTTGGTGTCTGAAGCTCTGTGTT; GCAAGATCGCCGACTTCGGGCTCGCTCGGATCATCGATAGCGAATATACTGCCCAAGAGGGTGCCAAGTT; CCCGATCAAATGGACCGCCCCCGAGGCCATCCACTTCGGCGTCTTCACAATTAAGGCTGACGTGTGGTCC; TTTGGGGTCCTGCTTATGGAGGTGGTTACATACGGTCGGGTTCCCTACCCTGGTATGTCCAACCCCGAGG; TGATTCGAAACCTTGAGAGGGGCTATCGGATGCCCCGGCCCGACACCTGCCCACCCGAGCTGTATAGAGG; CGTTATTGCTGAATGCTGGCGGTCCCGGCCTGAGGAACGCCCAACATTCGAGTTTTTGCAATCAGTGCTG; GAAGATTTCTATACCGCCACTGAACGGCAGTACGAACTGCAGCCC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207123 representing NM_001715; Red=Cloning site Green=Tags(s); ; MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYT; AMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLA; PINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGD; GLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEA; FLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAY; IERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWS; FGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVL; EDFYTATERQYELQP; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001715
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001715.2, NP_001706.2
• RefSeq Size: 2215 bp
• RefSeq ORF: 1518 bp
• Locus ID: 640
• UniProt ID: P51451
• Cytogenetics: 8p23.1
• Domains: pkinase, SH2, SH3, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Summary: This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]