Complement factor B (CFB) (NM_001710) Human Untagged Clone

SKU SC119051

CFB (untagged)-Human complement factor B (CFB)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Complement factor B
• Synonyms: AHUS4; ARMD14; BF; BFD; CFAB; CFBD; FB; FBI12; GBG; H2-Bf; PBF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119051 sequence for NM_001710 edited (data generated by NextGen Sequencing); ATGGGGAGCAATCTCAGCCCCCAACTCTGCCTGATGCCCTTTATCTTGGGCCTCTTGTCT; GGAGGTGTGACCACCACTCCATGGTCTTTGGCCCGGCCCCAGGGATCCTGCTCTCTGGAG; GGGGTAGAGATCAAAGGCGGCTCCTTCCGACTTCTCCAAGAGGGCCAGGCACTGGAGTAC; GTGTGTCCTTCTGGCTTCTACCCGTACCCTGTGCAGACACGTACCTGCAGATCTACGGGG; TCCTGGAGCACCCTGAAGACTCAAGACCAAAAGACTGTCAGGAAGGCAGAGTGCAGAGCA; ATCCACTGTCCAAGACCACACGACTTCGAGAACGGGGAATACTGGCCCCGGTCTCCCTAC; TACAATGTGAGTGATGAGATCTCTTTCCACTGCTATGACGGTTACACTCTCCGGGGCTCT; GCCAATCGCACCTGCCAAGTGAATGGCCGGTGGAGTGGGCAGACAGCGATCTGTGACAAC; GGAGCGGGGTACTGCTCCAACCCGGGCATCCCCATTGGCACAAGGAAGGTGGGCAGCCAG; TACCGCCTTGAAGACAGCGTCACCTACCACTGCAGCCGGGGGCTTACCCTGCGTGGCTCC; CAGCGGCGAACGTGTCAGGAAGGTGGCTCTTGGAGCGGGACGGAGCCTTCCTGCCAAGAC; TCCTTCATGTACGACACCCCTCAAGAGGTGGCCGAAGCTTTCCTGTCTTCCCTGACAGAG; ACCATAGAAGGAGTCGATGCTGAGGATGGGCACGGCCCAGGGGAACAACAGAAGCGGAAG; ATCGTCCTGGACCCTTCAGGCTCCATGAACATCTACCTGGTGCTAGATGGATCAGACAGC; ATTGGGGCCAGCAACTTCACAGGAGCCAAAAAGTGTCTAGTCAACTTAATTGAGAAGGTG; GCAAGTTATGGTGTGAAGCCAAGATATGGTCTAGTGACATATGCCACATACCCCAAAATT; TGGGTCAAAGTGTCTGAAGCAGACAGCAGTAATGCAGACTGGGTCACGAAGCAGCTCAAT; GAAATCAATTATGAAGACCACAAGTTGAAGTCAGGGACTAACACCAAGAAGGCCCTCCAG; GCAGTGTACAGCATGATGAGCTGGCCAGATGACGTCCCTCCTGAAGGCTGGAACCGCACC; CGCCATGTCATCATCCTCATGACTGATGGATTGCACAACATGGGCGGGGACCCAATTACT; GTCATTGATGAGATCCGGGACTTGCTATACATTGGCAAGGATCGCAAAAACCCAAGGGAG; GATTATCTGGATGTCTATGTGTTTGGGGTCGGGCCTTTGGTGAACCAAGTGAACATCAAT; GCTTTGGCTTCCAAGAAAGACAATGAGCAACATGCGTTCAAAGTCAAGGATATGGAAAAC; CTGGAAGATGTTTTCTACCAAATGATCGATGAAAGCCAGTCTCTGAGTCTCTGTGGCATG; GTTTGGGAACACAGGAAGGGTACCGATTACCACAAGCAACCATGGCAGGCCAAGATCTCA; GTCATTCGCCCTTCAAAGGGACACGAGAGCTGTATGGGGGCTGTGGTGTCTGAGTACTTT; GTGCTGACAGCAGCACATTGTTTCACTGTGGATGACAAGGAACACTCAATCAAGGTCAGC; GTAGGAGGGGAGAAGCGGGACCTGGAGATAGAAGTAGTCCTATTTCACCCCAACTACAAC; ATTAATGGGAAAAAAGAAGCAGGAATTCCTGAATTTTATGACTATGACGTTGCCCTGATC; AAGCTCAAGAATAAGCTGAAATATGGCCAGACTATCAGGCCCATTTGTCTCCCCTGCACC; GAGGGAACAACTCGAGCTTTGAGGCTTCCTCCAACTACCACTTGCCAGCAACAAAAGGAA; GAGCTGCTCCCTGCACAGGATATCAAAGCTCTGTTTGTGTCTGAGGAGGAGAAAAAGCTG; ACTCGGAAGGAGGTCTACATCAAGAATGGGGATAAGAAAGGCAGCTGTGAGAGAGATGCT; CAATATGCCCCAGGCTATGACAAAGTCAAGGACATCTCAGAGGTGGTCACCCCTCGGTTC; CTTTGTACTGGAGGAGTGAGTCCCTATGCTGACCCCAATACTTGCAGAGGTGATTCTGGC; GGCCCCTTGATAGTTCACAAGAGAAGTCGTTTCATTCAAGTTGGTGTAATCAGCTGGGGA; GTAGTGGATGTCTGCAAAAACCAGAAGCGGCAAAAGCAGGTACCTGCTCACGCCCGAGAC; TTTCACATCAACCTCTTTCAAGTGCTGCCCTGGCTGAAGGAGAAACTCCAAGATGAGGAT; TTGGGTTTTCTATAA; ; Clone variation with respect to NM_001710.5; 450 a=>g;1355 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001710 unedited; AACCTCGAGATTTGTAAACCCACCTCCTATAGGCGGAAAAACACTTCGCACGAGGTCTAG; GTCTGGAGTTTCAGCTTGGACACTGAGCCAAGCAGACAAGCAAAGCAAGCCAGGACACAC; CATCCTGCCCCAGGCCCAGCTTCTCTCCTGCCTTCCAACGCCATGGGGAGCAATCTCAGC; CCCCAACTCTGCCTGATGCCCTTTATCTTGGGCCTTCTGTTTGGAGGTGTGACCACCACT; CCATGGTCTTTGGCCCGGCCCCAGGGATCCTGCTCTCTGGAGGGGGTAGAGATCAAAGGC; GGCTCCTTCCGACTTCTCCAAGAGGGCCAGGCACTGGAGTACGTGTGTCCTTCTGGCTTC; TACCCGTACCCTGTGCAGACACGTACCTGCAGATCTACGGGGTCCTGGAGCACCCTGAAG; ACTCAAGACCAAAAGACTGTCAGGAAGGCAGAGTGCAGAGCAATCCACTGTCCAAGACCA; CACGACTTCGAGAACGGGGAATACTGGCCCCGGTCTCCCTACTACAATGTGAGTGATGAG; ATCTCTTTCCACTGCTATGACGGTTACACTCTCCGGGGCTCTGCCAATCGCACCTGCCAA; GTGAATGGCCGGTGGAGTGGGCAGACAGCGATCTGTGACAACGGAGCGGGGTACTGCTCC; AACCCGGGCATCCCCATTGGCACAAGGAAAGTGGGCAGCCAGTACCGCCTTGAAGACAGC; GTCACCTACCACTGCAGCCGGAGGCTTACCCTGCGTGGCTCCCCAGCGCGAACGTGTCAA; GAAGGTGGCTCTTGNAGCGGGACAGAGCCTTCCTGCCAAGACTCCTTCATGTACGACACC; CCTCAAGAGTGGCCGAAACTTTTCTGTCTTCCCTGACAGAGACATT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001710 unedited; TTGGACCGCGNGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTTGTCGCAGCT; GTTTTAATTCAATCCCACGCCCCTGTCCAGCAGGAAACCCCTTATAGAAAACCCAAATCC; TCATCTTGGAGTTTCTCCTTCAGCCAGGGCAGCACTTGAAAGAGGTTGATGTGAAAGTCT; CGGGCGTGAGCAGGTACCTGCTTTTGCCGCTTCTGGTTTTTGCAGACATCCACTACTCCC; CAGCTGATTACACCAACTTGAATGAAACGACTTCTCTTGTGAACTATCAAGGGGCCGCCA; GAATCACCTCTGCAAGTATTGGGGTCAGCATAGGGACTCACTCCTCCAGTACAAAGGAAC; CGAGGGGTGACCACCTCTGAGATGTCCTTGACTTTGTCATAGCCTGGGGCATATTGAGCA; TCTCTCTCACAGCTGCCTTTCTTATCCCCATTCTTGATGTAGACCTCCTTCCGAGTCAGC; TTTTTCTCCTCCTCAGACACAAACAGAGCTTTGATATCCTGTGCAGGGAGCAGCTCTTCC; TTTTGTTGCTGGCAAGTGGTAGTTGGAGGAAGCCTCAAAGCTCGAGTTGTTCCCTCGGTG; CAGGGGAGACAAATGGGCCTGATAGTCTGGCCATATTTCAGCTTATTCTTGAGCTTGATC; AGGGCAACGTCATAGTCATAAAATTCAGGAATTCCTGCTTCTTTTTTCCCATTAATGNTG; TAGTTGGGGGTGAAATAGGACTACTTCTATCTNCAGGTCCCGCTTCTCCCCTCCTACGCT; GACCTTTGATGAGTGTTCCTTGTCATCACAGTGAAACAATGTGCTGCTGTCAGCACAAGT; ACTCAGACACCACANCCCCATACAGCTCTCGTGTCCTTTTGAGGGCGATGACTGANATCT; CGCCTGCCATGGTGCCTGNGGTAATCGNACCTTCCTTGGTTCCAAACATGCCCAAAGACT; TAAAACTGCTTCATGATCATTGTAGAAAAATCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001710
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001710.4, NP_001701.2
• RefSeq Size: 2574 bp
• RefSeq ORF: 2295 bp
• Locus ID: 629
• UniProt ID: P00751
• Cytogenetics: 6p21.33
• Domains: CCP, Tryp_SPc, VWA
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Summary: This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008]