BCL7B (NM_001707) Human Untagged Clone

SKU SC119050

BCL7B (untagged)-Human B-cell CLL/lymphoma 7B (BCL7B), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: BCL7B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119050 sequence for NM_001707 edited (data generated by NextGen Sequencing); ATGTCGGGCCGGTCGGTCCGGGCGGAGACCCGCAGCCGGGCCAAGGACGACATCAAGAAG; GTGATGGCGGCCATCGAGAAAGTGCGGAAATGGGAGAAGAAGTGGGTGACTGTGGGTGAC; ACGTCCCTGAGGATATTTAAGTGGGTTCCTGTGACAGACAGCAAGGAGAAAGAAAAGTCA; AAATCGAACAGTTCAGCAGCCCGAGAACCTAATGGCTTTCCTTCTGATGCCTCAGCCAAT; TCCTCTCTCCTTCTTGAATTCCAGGACGAAAACAGCAACCAGAGTTCCGTGTCTGACGTC; TATCAGCTTAAGGTGGACAGCAGCACCAACTCAAGCCCCAGCCCCCAGCAGAGTGAGTCC; CTGAGCCCAGCACACACCTCCGACTTCCGCACGGATGACTCCCAGCCCCCAACGCTGGGC; CAGGAGATCCTGGAGGAGCCCTCCCTGCCCTCCTCGGAAGTTGCTGATGAACCTCCTACC; CTCACCAAGGAAGAACCAGTTCCACTAGAGACACAGGTCGTTGAGGAAGAGGAAGACTCA; GGTGCCCCGCCCCTGAAGCGCTTCTGTGTGGACCAACCCACAGTGCCGCAGACGGCGTCA; GAAAGCTAG; ; Clone variation with respect to NM_001707.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001707 unedited; TTCAGATTTGTATACGACTCCTATAGGCGGCCGCGCAATTCGCACGAGGTGCCCCGGCCC; CCGCCGCTCCCGCCGCCGCCATGTCGGGCCGGTCGGTCCGGGCGGAGACCCGCAGCCGGG; CCAAGGACGACATCAAGAAGGTGATGGCGGCCATCGAGAAAGTGCGGAAATGGGAGAAGA; AGTGGGTGACTGTGGGTGACACGTCCCTGAGGATATTTAAGTGGGTTCCTGTGACAGACA; GCAAGGAGAAAGAAAAGTCAAAATCGAACAGTTCAGCAGCCCGAGAACCTAATGGCTTTC; CTTCTGATGCCTCAGCCAATTCCTCTCTCCTTCTTGAATTCCAGGACGAAAACAGCAACC; AGAGTTCCGTGTCTGACGTCTATCAGCTTAAGGTGGACAGCAGCACCAACTCAAGCCCCA; GCCCCCAGCAGAGTGAGTCCCTGAGCCCAGCACACACCTCCGACTTCCGCACGGATGACT; CCCAGCCCCCAACGCTGGGCCAGGAGATCCTGGAGGAGCCCTCCCTGCCCTCCTCGGAAG; TTGCTGATGAACCTCCTACCCTCACCAAGGAAGAACCAGTTCCACTAGAGACACAGGTCG; TTGAGGAAGAGGAAGACTCANGTGCCCCGCCCCTGAAGCGCTTCTGTGTGGACCAACCCA; CAGTGCCGCAGACGGCGTCAGAAAGCTAGCACCATCCCCGCCCTCCGCCTCCTGGCCCTG; CCTCTATTTATTGCATTCTGGTTCTGCCCGCGCCGCGTTGCTGGGGTAAGGCCAGCACCT; GGGTCAAGAGCCTGCACCATGACCCTTCCGGCCTGNAANGCTTGCGTTAGACTTGGGGCT; GTACCATCATCTTCCTGACCCTGGCACCTGGTCTACTTGCTCCCGAAAGAGAGCCCTCAT; GTTTTTTTGACCCCCAGATG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001707 unedited; CCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACATATTTCAAACAGTT; TAATTTTAAAAATATTCTATTCAGTGCTAAAATATGTCTTCACTCACTGTTGCCCATTTC; CATTTTCTTAAACTTTTTTGAAACAAAAAATAAAATCACAAAGTTCAATTCAACATGCAG; ATTTCAAAGAAAAGGGAGCTTTCTAGAAACTGAGCTGAAGGGGTACTGTGGGGGATGGGT; ACTGTTGTCAGAACCTCAAATTCTTGCTGGGGCAGTTTGTGACAAGAGGCAGGGATGCTG; GAATGACAGGTAGAGGTGAGAACAAAGCTGCGTGTGTGCAGATGTGTGTGCTGAGGGCAG; GGGATGGGGACGGCGGCCACAAAGGGCTGTCAGCAGCTAGGGTGCCCGTGAGAGGTAGGA; CCACCTCAGGCATGGGCAGAGCTGGCAGGACAGCAAGAAATCTAGACGGACTCTCTGCCC; TTGTGTGGAGAAAAGCCCTTCCCTTCCCTCCAATGTCTCTACCCAGTCCTCCAACAGCAG; CCTACAGGGCCCCCAGTCCTAGGGGAAAGTCCGGGAGTCTACAGTCTCCGTTCAAGTTCA; AGTAACCTGAGGCACGCTCTCTTCTAGAAGGTGCCTTCCACATCTCTCTCTTCTCGCAGA; AGCTGCCAGCAGCTCCGTCCAGAGATTCTGAGCAGAGACTGCTGCCTGGAGGTCACAGAT; GAATCTTGGGGTGGCCGATGCTCCAGCCAACTTGGGTGCAAAAAGACATGAGCGCTCCTC; TTCTCGGAGCAGTANACACAGTGCAGGTCAAGAANATGATCTACACCCCAGTCTACGCAG; CCTCCACCCGAAGCTAATGGTGCAGCTCTTGACCAGGGTTGCCTTAACCCAAACGCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001707
• Insert Size: 1710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001707.2, NP_001698.2
• RefSeq Size: 1690 bp
• RefSeq ORF: 609 bp
• Locus ID: 9275
• UniProt ID: Q9BQE9
• Cytogenetics: 7q11.23
• Domains: BCL_N
• Summary: This gene encodes a member of the BCL7 family including BCL7A, BCL7B and BCL7C proteins. This member is BCL7B, which contains a region that is highly similar to the N-terminal segment of BCL7A or BCL7C proteins. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. This gene is located at a chromosomal region commonly deleted in Williams syndrome. This gene is highly conserved from C. elegans to human. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2010]; Transcript Variant: This variant (1) encodes isoform 1.