EEF1A2 (NM_001958) Human Untagged Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | EEF1A2 |
| Synonyms | DEE33; EEF1AL; EF-1-alpha-2; EF1A; EIEE33; HS1; MRD38; STN; STNL |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_001958 |
| Insert Size | 1890 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001958.2, NP_001949.1 |
| RefSeq Size | 1841 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 1917 |
| UniProt ID | Q05639 |
| Cytogenetics | 20q13.33 |
| Domains | GTP_EFTU, GTP_EFTU_D2, GTP_EFTU_D3 |
| Protein Families | Druggable Genome |
| Summary | This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. provided by RefSeq, Mar 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001958" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210716 | EEF1A2 (Myc-DDK-tagged)-Human eukaryotic translation elongation factor 1 alpha 2 (EEF1A2) | 10 ug |
$686.00
|
|
| RC210716L1 | Lenti ORF clone of Human eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC210716L2 | Lenti ORF clone of Human eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mGFP tagged | 10 ug |
$986.00
|
|
| RC210716L3 | Lenti ORF clone of Human eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC210716L4 | Lenti ORF clone of Human eukaryotic translation elongation factor 1 alpha 2 (EEF1A2), mGFP tagged | 10 ug |
$986.00
|
|
| RG210716 | EEF1A2 (tGFP-tagged) - Human eukaryotic translation elongation factor 1 alpha 2 (EEF1A2) | 10 ug |
$886.00
|
|
Citations
Protumorigenic role of Timeless in hepatocellular carcinoma
Elgohary, Pellegrino, Neumann et al
Int. J. Oncol. (2014)
Elgohary, Pellegrino, Neumann et al
Int. J. Oncol. (2014)
Cited by 46
AI Summary: OriGene’s EEF1A2 cDNA in the pcmV6-XL5 vector was used for transient transfection experiments to study the role of EEF1A2 in hepatocellular carcinoma cell migration and its regulation by Timeless.
Snippet: transient transfec- tion experiments of hep3B cells with EEF1A2 cDNA in pcmV6-Xl5 vector (origene technologies, Rockville, mD, USA) was performed following the manufacturer's protocol using Fugene hD transfection reagent (Promega, mannheim, Germany). ... Cite SaveSaving
EEF1A2 inactivates p53 via PI3K/AKT/mTOR-dependent stabilization of MDM4 in hepatocellular carcinoma
Pellegrino, Calvisi, Neumann et al
Hepatology (2013)
Pellegrino, Calvisi, Neumann et al
Hepatology (2013)
Cited by 104
Showing 1-2 of 2 papers.
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