FGF2 (NM_002006) Human Untagged Clone

SKU SC118884

FGF2 (untagged)-Human fibroblast growth factor 2 (basic) (FGF2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FGF2
• Synonyms: BFGF; FGF-2; FGFB; HBGF-2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002006 edited; GAATTCGGCACGAGGGGAGGGAGGAGAACTGGGGGCGCGGGAGGCTGGTGGGTGTGGGGG; GTGGAGATGTAGAAGATGTGACGCCGCGGCCCGGCGGGTGCCAGATTAGCGGACGCGGTG; CCCGCGGTTGCAACGGGATCCCGGGCGCTGCAGCTTGGGAGGCGGCTCTCCCCAGGCGGC; GTCCGCGGAGACACCCATCCGTGAACCCCAGGTCCCGGGCCGCCGGCTCGCCGCGCACCA; GGGGCCGGCGGACAGAAGAGCGGCCGAGCGGCTCGAGGCTGGGGGACCGCGGGCGCGGCC; GCGCGCTGCCGGGCGGGAGGCTGGGGGGCCGGGGCCGGGGCCGTGCCCCGGAGCGGGTCG; GAGGCCGGGGCCGGGGCCGGGGGACGGCGGCTCCCCGCGCGGCTCCAGCGGCTCGGGGAT; CCCGGCCGGGCCCCGCAGGGACCATGGCAGCCGGGAGCATCACCACGCTGCCCGCCTTGC; CCGAGGATGGCGGCAGCGGCGCCTTCCCGCCCGGCCACTTCAAGGACCCCAAGCGGCTGT; ACTGCAAAAACGGGGGCTTCTTCCTGCGCATCCACCCCGACGGCCGAGTTGACGGGGTCC; GGGAGAAGAGCGACCCTCACATCAAGCTACAACTTCAAGCAGAAGAGAGAGGAGTTGTGT; CTATCAAAGGAGTGTGTGCTAACCGTTACCTGGCTATGAAGGAAGATGGAAGATTACTGG; CTTCTAAATGTGTTACGGATGAGTGTTTCTTTTTTGAACGATTGGAATCTAATAACTACA; ATACTTACCGGTCAAGGAAATACACCAGTTGGTATGTGGCACTGAAACGAACTGGGCAGT; ATAAACTTGGATCCAAAACAGGACCTGGGCAGAAAGCTATACTTTTTCTTCCAATGTCTG; CTAAGAGCTGATTTTAATGGCCACATCTAATCTCATTTCACATGAAAGAAGAAGTATATT; TTAGAAATTTGTTAATGAGAGTAAAAGAAAATAAATGTGTATAGCTCAGTTTGGATAATT; GGTCAAACAATTTTTTATCCAGTAGTAAAATATGTAACCATTGTCCCAGTAAAGAAAAAT; AACAAAAGTTGTAAAATGTATATTCTCCCTTTTATATTGCATCTGCTGTTACCCAGTGAA; GCTTACCTAGAGCAATGATCTTTTTCACGCATTTGCTTTATTCGAAAAGAGGCTTTTAAA; ATGTGCATGTTTAGAAACAAAATTTCTTCATGGAAATCATATACATTAGAAAATCACAGT; CAGATGTTTAATCAATCCAAAATGTCCACTATTTCTTATGTCATTCGTTAGTCTACATGT; TTCTAAACATATAAATGTGAATTTAATCAATTCCTTTCATAGTTTTATAATTCTCTGGCA; GTTCCTTATGATAGAGTTTATAAAACAGTCCTGTGTAAACTGCTGGAAGTTCTTCCACAG; TCAGGTCAATTTTGTCAAACCCTTCTCTGTACCCATACAGCAGCAGCCTAGCAACTCTGC; TGGTGATGGGAGTTGTATTTTCAGTCTTCGCCAGGTCATTGAGATCCATCCACTCACATC; TTAAGCATTCTTCCTGGCAAAAATTTATGGTGAATGAATATGGCTTTAGGCGGCAGATGA; TATACATATCTGACTTCCCAAAAGCTCCAGGATTTGTGTGCTGTTGCCGAATACTCAGGA; CGGACCTGAATTCTGATTTTATACCAGTCTCTTCAAAAACTTCTCGAACCGCTGTGTCTC; CTACGTAAAAAAAGAGATGTACAAATCAATAATAATTACACTTTTAGAAACTGTATCATC; AAAGATTTTCAGTTAAAGTAGCATTATGTAAAGGCTCAAAACATTACCCTAACAAAGTAA; AGTTTTCAATACAAATTCTTTGCCTTGTGGATATCAAGAAATCCCAAAATATTTTCTTAC; CACTGTAAATTCAAGAAGCTTTTGAAATGCTGAATATTTCTTTGGCTGCTACTTGGAGGC; TTATCTACCTGTACATTTTTGGGGTCAGCTCTTTTTAACTTCTTGCTGCTCTTTTTCCCA; AAAGGTAAAAATATAGATTGAAAAGTTAAAACATTTTGCATGGCTGCAGTTCCTTTGTTT; CTTGAGATAAGATTCCAAAGAACTTAGATTCATTTCTTCAACACCGAAATGCTGGAGGTG; TTTGATCAGTTTTCAAGAAACTTGGAATATAAATAATTTTATAATTCAACAAAGGTTTTC; ACATTTTATAAGGTTGATTTTTCAATTAAATGCAAATTTATGTGGCAGGATTTTTATTGC; CATTAACATATTTTTGTGGCTGCTTTTTCTACACATCCAGATGGTCCCTCTAACTGGGCT; TTCTCTAATTTTGTGATGTTCTGTCATTGTCTCCCAAAGTATTTAGGAGAAGCCCTTTAA; AAAGCTGCCTTCCTCTACCACTTTGCTGGAAAGCTTCACAATTGTCACAGACAAAGATTT; TTGTTCCAATACTCGTTTTGCCTCTATTTTTCTTGTTTGTCAAATAGTAAATGATATTTG; CCCTTGCAGTAATTCTACTGGTGAAAAACATGCAAAGAAGAGGAAGTCACAGAAACATGT; CTCAATTCCCATGTGCTGTGACTGTAGACTGTCTTACCATAGACTGTCTTACCCATCCCC; TGGATATGCTCTTGTTTTTTCCCTCTAATAGCTATGGAAAGATGCATAGAAAGAGTATAA; TGTTTTAAAACATAAGGCATTCGTCTGCCATTTTTCAATTACATGCTGACTTCCCTTACA; ATTGAGATTTGCCCATAGGTTAAACATGGTTAGAAACAACTGAAAGCATAAAAGAAAAAT; CTAGGCCGGGTGCAGTGGCTCATGCCTATATTCCCTGCACTTTGGGAGGCCAAAGCAGGA; GGATCGCTTGAGCCCAGGAGTTCAAGACCAACCTGGTGAAACCCCGTCTCTACAAAAAAA; CACAAAAAATAGCCAGGCATGGTGGCGTGTACATGTGGTCTCAGATACTTGGGAGGCTGA; GGTGGGAGGGTTGATCACTTGAGGCTGAGAGGTCAAGGTTGCAGTGAGCCATAATCGTGC; CACTGCAGTCCAGCCTAGGCAACAGAGTGAGACTTTGTCTCAAAAAAAGAGAAATTTTCC; TTAATAAGAAAAGTAATTTTTACTCTGATGTGCAATACATTTGTTATTAAATTTATTATT; TAAGATGGTAGCACTAGTCTTAAATTGTATAAAATATCCCCTAACATGTTTAAATGTCCA; TTTTTATTCATTATGCTTTGAAAAATAATTATGGGGAAATACATGTTTGTTATTAAATTT; ATTATTAAAGATAGTAGCACTAGTCTTAAATTTGATATAACATCTCCTAACTTGTTTAAA; TGTCCATTTTTATTCTTTATGTTTGAAAATAAATTATGGGGATCCTATTTAGCTCTTAGT; ACCACTAATCAAAAGTTCGGCATGTAGCTCATGATCTATGCTGTTTCTATGTCGTGGAAG; CACCGGATGGGGGTAGTGAGCAAATCTGCCCTGCTCAGCAGTCACCATAGCAGCTGACTG; AAAATCAGCACTGCCTGAGTAGTTTTGATCAGTTTAACTTGAATCACTAACTGACTGAAA; ATTGAATGGGCAAATAAGTGCTTTTGTCTCCAGAGTATGCGGGAGACCCTTCCACCTCAA; GATGGATATTTCTTCCCCAAGGATTTCAAGATGAATTGAAATTTTTAATCAAGATAGTGT; GCTTTATTCTGTTGTATTTTTTATTATTTTAATATACTGTAAGCCAAACTGAAATAACAT; TTGCTGTTTTATAGGTTTGAAGAACATAGGAAAAACTAAGAGGTTTTGTTTTTATTTXXX; XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002006 unedited; ACATTTTGTATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGGAGGGAGGAG; AACTGGGGGCGCGGGAGGCTGGTGGGTGTGGGGGGTGGAGATGTAGAAGATGTGACGCCG; CGGCCCGGCGGGTGCCAGATTAGCGGACGCGGTGCCCGCGGTTGCAACGGGATCCCGGGC; GCTGCAGCTTGGGAGGCGGCTCTCCCCAGGCGGCGTCCGCGGAGACACCCATCCGTGAAC; CCCAGGTCCCGGGCCGCCGGCTCGCCGCGCACCAGGGGCCGGCGGACAGAAGAGCGGCCG; AGCGGCTCGAGGCTGGGGGACCGCGGGCGCGGCCGCGCGCTGCCGGGCGGGAGGCTGGGG; GGCCGGGGCCGGGGCCGTGCCCCGGAGCGGGTCGGAGGCCGGGGCCGGGGCCGGGGGACG; GCGGCTCCCCGCGCGGCTCCAGCGGCTCGGGGATCCCGGCCGGGCCCCGCAGGGACCATG; GCAGCCGGGAGCATCACCACGCTGCCCGCCTTGCCCGAGGATGGCGGCAGCGGCGCCTTC; CCGCCCGGCCACTTCAAGGACCCCAAGCGGCTGTACTGCAAAAACGGNGGCTTCTTCCTG; CGCATCCACCCCGACGGCCGAGTTGACTGGGTCCGGGAGAAGAGCGACCCTCACATCAAG; CTACAACTTTAAGCAGAAGAGAGAGGAGTTGTGTCTATCAAATGAGTGTGTGCTAACCGT; TACCTGGCTATGAACGAAGAATGGAAGATTACTGGCTTCTAAAGTGTTACCGATGAGTGT; TTCTCTTTTCACCGATCGGAATCTATTACTACAATCTTTCCCGTCAAGGAATACACCATT; TGGTTTGTGGCACTGAACAAACTGCGCGCTTAACTCGGATCCAAAAGGAACTGGGCAGAA; AGCTTTCTTTTTCTTCCAAGTTGCTAAAACCTATTTAATGGCCCCCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002006 unedited; TGTTGTTTTTCATCCTAATTCTATTATTAAATATAACAAAATAGAAATAACAAATATAAA; CTCCATTTCATTATTGTCCTGTAACTAAACAAAACAATACAACATATTTAAACATATCTC; TTCATCAGCAAAAATAAAAACAAAACCTCTTAGTTTTTCCTATGTTCTTCAAACCTATAA; AACAGCAAATGTTATTTCAGTTTGGCTTACAGTATATTAAAATAATAAAAAATACAACAG; AATAAAGCACACTATCTTGATTAAAAATTTCAATTCATCTTGAAATCCTTGGGGAAGAAA; TATCCATCTTGAGGTGGAAGGGTCTCCCGCATACTCTGGAGACAAAAGCACTTATTTGCC; CATTCAATTTTCAGTCAGTTAGTGATTCAAGTTAAACTGATCAAAACTACTCAGGCAGTG; CTGATTTTCAGTCAGCTGCTATGGTGACTGCTGAGCAGGGCAGATTTGCTCACTACCCCC; ATCCGGTGCTTCCACGACATAGAAACAGCATAGATCATGAGCTACATGCCGAACTTTTGA; TTAGTGGTACTAAGAGCTAAATAGGATCCCCATAATTTATTTTCAAACATAAAGAATAAA; AATGGACATTTAAACAAGTTAGGAGATGTTATATCAAATTTAAGACTAGTGCTACTATCT; CTAATAATAAATTTAATAACAAACATGTATCTCCCCATAATTATCTTTCACAGCATAATG; AATAAAAATGGACATTTAAACATGTTAGGGGATATTTTATACAATTTAAGACTAGTGCTA; CCATCTTAAATATAAATTTATAACACTGTATTGCACATCAGAGTAAAAATACTTTTCTAT; AAGGGAAATTTCTCTTTTTTGAGACAAGTCTACTCTGTGGCTAGCCTGACTGCAATGCAC; GATATTGCTCACTGCACTCTGACTCTCAGCCCCAGGATTACCCTTCCACTCACGCTCCAA; GGATTGNAACACATGCCCNCCACT;
• Restriction Sites: Please inquire
• ACCN: NM_002006
• Insert Size: 867 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002006.3, NP_001997.4
• RefSeq Size: 6803 bp
• RefSeq ORF: 867 bp
• Locus ID: 2247
• UniProt ID: P09038
• Cytogenetics: 4q28.1
• Domains: FGF
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton
• Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]; Transcript Variant: This transcript may encode multiple distinct isoforms through the use of alternative translation start codons (PMID: 17131363). This record represents use of the 5' most non-AUG start codon and encodes the longest (34-kDa) isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.