FES (NM_002005) Human Untagged Clone

SKU SC118883

FES (untagged)-Human feline sarcoma oncogene (FES), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: FES
• Synonyms: FPS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118883 sequence for NM_002005 edited (data generated by NextGen Sequencing); ATGGGCTTCTCTTCCGAGCTGTGCAGCCCCCAGGGCCACGGGGTCCTGCAGCAAATGCAG; GAGGCCGAGCTTCGTCTACTGGAGGGCATGAGAAAGTGGATGGCCCAGCGGGTCAAGAGT; GACAGGGAGTATGCAGGACTGCTTCACCACATGTCCCTGCAGGACAGTGGGGGCCAGAGC; CGGGCCATCAGCCCTGACAGCCCCATCAGTCAGTCCTGGGCTGAGATCACCAGCCAAACT; GAGGGCCTGAGCCGCTTGCTGCGGCAGCACGCAGAGGATCTGAACTCAGGGCCCCTGAGC; AAGCTGAGCCTGCTCATCCGGGAACGGCAGCAGCTTCGCAAGACCTACAGCGAGCAGTGG; CAGCAGCTGCAGCAGGAGCTCACCAAGACCCACAGCCAGGACATTGAGAAGCTGAAGAGC; CAGTACCGAGCTCTGGCACGGGACAGTGCCCAAGCCAAGCGCAAGTACCAGGAGGCCAGC; AAAGACAAGGACCGTGACAAGGCTAAGGACAAGTATGTGCGCAGCCTGTGGAAGCTCTTT; GCTCACCACAACCGCTATGTGCTGGGCGTGCGGGCTGCGCAGCTACACCACCAGCACCAC; CACCAGCTCCTGCTGCCCGGCCTGCTGCGGTCACTGCAGGACCTGCACGAGGAGATGGCT; TGCATCCTGAAGGAGATCCTGCAGGAATACCTGGAGATTAGCAGCCTGGTGCAGGATGAG; GTGGTGGCCATTCACCGGGAGATGGCTGCAGCTGCTGCCCGCATCCAGCCTGAGGCTGAG; TACCAAGGCTTCCTGCGACAGTATGGGTCCGCACCTGACGTCCCACCCTGTGTCACGTTC; GATGAGTCACTGCTTGAGGAGGGTGAACCGCTGGAGCCTGGGGAGCTCCAGCTGAACGAG; CTGACTGTGGAGAGCGTGCAGCACACGCTGACCTCAGTGACAGATGAGCTGGCTGTGGCC; ACCGAGATGGTGTTCAGGCGGCAGGAGATGGTTACGCAGCTGCAACAGGAGCTCCGGAAT; GAAGAGGAGAACACCCACCCCCGGGAGCGGGTGCAGCTGCTGGGCAAGAGGCAAGTGCTG; CAAGAAGCACTGCAGGGGCTGCAGGTAGCGCTGTGCAGCCAGGCCAAGCTGCAGGCCCAG; CAGGAGTTGCTGCAGACCAAGCTGGAGCACCTGGGCCCCGGCGAGCCCCCGCCTGTGCTG; CTCCTGCAGGATGACCGCCACTCCACGTCGTCCTCGGAGCAGGAGCGAGAGGGGGGAAGG; ACACCCACGCTGGAGATCCTTAAGAGCCACATCTCAGGAATCTTCCGCCCCAAGTTCTCG; CTCCCTCCACCGCTGCAGCTCATTCCGGAGGTGCAGAAGCCCCTGCATGAGCAGCTGTGG; TACCACGGGGCCATCCCGAGGGCAGAGGTGGCTGAGCTGCTGGTGCACTCTGGGGACTTC; CTGGTGCGGGAGAGCCAGGGCAAGCAGGAGTACGTGCTGTCGGTGCTGTGGGATGGTCTG; CCCCGGCACTTCATCATCCAGTCCTTGGATAACCTGTACCGACTGGAAGGGGAAGGCTTT; CCTAGCATTCCTTTGCTCATCGACCACCTACTGAGCACCCAGCAGCCCCTCACCAAGAAG; AGTGGTGTTGTCCTGCACAGGGCTGTGCCCAAGGACAAGTGGGTGCTGAACCATGAGGAC; CTGGTGTTGGGTGAGCAGATTGGACGGGGGAACTTTGGCGAAGTGTTCAGCGGACGCCTG; CGAGCCGACAACACCCTGGTGGCGGTGAAGTCTTGTCGAGAGACGCTCCCACCTGACCTC; AAGGCCAAGTTTCTACAGGAAGCGAGGATCCTGAAGCAGTACAGCCACCCCAACATCGTG; CGTCTCATTGGTGTCTGCACCCAGAAGCAGCCCATCTACATCGTCATGGAGCTTGTGCAG; GGGGGCGACTTCCTGACCTTCCTCCGCACGGAGGGGGCCCGCCTGCGGGTGAAGACTCTG; CTGCAGATGGTGGGGGATGCAGCTGCTGGCATGGAGTACCTGGAGAGCAAGTGCTGCATC; CACCGGGACCTGGCTGCTCGGAACTGCCTGGTGACAGAGAAGAATGTCCTGAAGATCAGT; GACTTTGGGATGTCCCGAGAGGAAGCCGATGGGGTCTATGCAGCCTCAGGGGGCCTCAGA; CAAGTCCCCGTGAAGTGGACCGCACCTGAGGCCCTTAACTACGGCCGCTACTCCTCCGAA; AGCGACGTGTGGAGCTTTGGCATCTTGCTCTGGGAGACCTTCAGCCTGGGGGCCTCCCCC; TATCCCAACCTCAGCAATCAGCAGACACGGGAGTTTGTGGAGAAGGGGGGCCGTCTGCCC; TGCCCAGAGCTGTGTCCTGATGCCGTGTTCAGGCTCATGGAGCAGTGCTGGGCCTATGAG; CCTGGGCAGCGGCCCAGCTTCAGCACCATCTACCAGGAGCTGCAGAGCATCCGAAAGCGG; CATCGGTGA; ; Clone variation with respect to NM_002005.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002005 unedited; TCATAATTTGTAATACACTACTATAGGCGGCCGCGAATTCGGCACCAGCCCAAGAGCAGC; TGCCCGTGCGGGTACCTCTAGCCCCGGGGCCTGGAGGAGCGGTGGGAGCTGGGGGCGCGG; CAGGCAGGGGCAGAGCAGGCGTTCCGAGGGCCAGAGACCCACCCAGAACAGCACTATGGG; CTTCTCTTCCGAGCTGTGCAGCCCCCAAGGCCACGGGGTCCTGCAGCAAATGCAGGAGGC; CGAGCTTCGTCTACTGGAGGGCATGAGAAAGTGGATGGCCCAGCGGGTCAAGAGTGACAA; GGGAGTATGCAGGACTGCTTCACCACATGTCCCTGCAGGACAGTGGGGGCCAGATCCGGG; CCATCAGCCCTGACAGCCCCATCAGTCAGTCCTGGGCTGAGATCAACCAGCCAAACTGAG; GGCCTGAGCCGCTTGCTGCGGCAGCACGCAGAAGATCTGAACTCAGGGCCCCTGAGCAAG; CTGAGCCTGCTCATCCGGGAACGGCAGCAGCTTCGCAAGAACTACAGCGAGCAGTGGCAG; CAGCTGCAGCAGGAGCTCACCAAGACCCACAGCCAGGACATTGAGAAGCTGAAGAACCAG; TACCGAGCTCTGGCACGGGACAGTGCCCAAGCCAAGCGCAAGTACCATGAGGCCAGCAAA; ACAANGACCGTGACAAGGCTAAGACAAGTATGTGCGCAGCCTGTGGAATCTCTTTGCTCA; CCACAACCGCTATGTGCTTGGGCGTGCGGGCTGCGCATCTACACCACCATCAAACCACCA; TCTCTGCTGCCGGNCTGCTGCGGTACTGCAAGACTGCAGAANGAGAATGCTGATNCTGAA; AGAAANCTGCAGAATACTGGAAATTACANCTGTGCAGAATAAGGGGTGCCATTCACCGGA; AAGGCTGAGCTGCTGCCGCATCAACTGAGCTGATACAAGCTCTGGAAATATGGTCGAACT; GACTCACTTGGTAGTTNA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002005 unedited; GGACCCTAAGGGTGATGGCACCTNCCTGCNAGGTANATCACTGGCGNAGGGTCACAGGGC; ATGCCACCCGGGCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGT; TTTTTTTTTTTTTTTTTTCAGTGGGCACAAGTGGTTTTATTGTTTCTGCCCGGAAGAGGA; AGCCCTGGCAGCAGCAGGGACACAGAGAGGACACGGCTCTTTTTCCTGCCGGCAGTGTGG; ATGCTGGTGGCAGGAGTCCAGGACTGTGAAGAGCTGTCAGCATATGAGCTGGAGCCGCTG; ATGAGCTGCACCTAGGCCTGCAGAGGCCACCAGCTTGAGAAGGGGGTCCCAGCCTCACCG; ATGCCGCTTTCGGATGCTCTGCAGCTCCTGGTATATGGTGCTGAAGCTGGGCCGCTGCCC; AGGCTCATAGGCCCAGCTCTGCTCCATGAGCCTGAACACGGCATCAGGACACAGCTCTGG; GCAGGGCAGACGGCCCCCCTTCTCCACAAACTCCCGTGTCTGCTGATTGCTGAGGTTGGG; ATAGGGGGAGGCCCCCAGGCTGAAGGTCTCCCTTAGCAAGATGCCTAAGCTCCACATTTC; GCTTTCGGAGGAGTAGCGGCCGTAGTTAAGGGCCTCAAGTGCGGTCCACTTCACGGGGGA; CTTGTCTGAGGCCCCCTGATGCTGCCTAGACCCTATGGGCTTCCTCTCGGGACATCCCAT; AGTCACTGATCTTCAGGACATTCTTCTCTGTCCCCGGGCTTTTTCCGATCACCCAGGTCC; CGGTGGGTGCAACTTTTGCTCTTCCGGTTCTCCTTGCCGCCGTTTGTTTCCCCCCCCCTC; TGGTCGGTATTCTCACCCGCGGTCGGCCCCCCTTCTTCCGGNGGAAGGTAAGGATTCCCC; ACCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_002005
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002005.2, NP_001996.1
• RefSeq Size: 2762 bp
• RefSeq ORF: 2469 bp
• Locus ID: 2242
• UniProt ID: P07332
• Cytogenetics: 15q26.1
• Domains: FCH, pkinase, SH2, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance
• Summary: This gene encodes the human cellular counterpart of a feline sarcoma retrovirus protein with transforming capabilities. The gene product has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia but it is also involved in normal hematopoiesis as well as growth factor and cytokine receptor signaling. Alternative splicing results in multiple variants encoding different isoforms.[provided by RefSeq, Jan 2009]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).