Glutaredoxin 1 (GLRX) (NM_002064) Human Untagged Clone

SKU SC118879

GLRX (untagged)-Human glutaredoxin (thioltransferase) (GLRX), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Glutaredoxin 1
• Synonyms: GRX; GRX1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002064 edited; ATGGCTCAAGAGTTTGTGAACTGCAAAATCCAGCCTGGGAAGGTGGTTGTGTTCATCAAG; CCCACCTGCCCGTACTGCAGGAGGGCCCAAGAGATCCTCAGTCAATTGCCCATCAAACAA; GGGCTTCTGGAATTTGTCGATATCACAGCCACCAACCACACTAACGAGATTCAAGATTAT; TTGCAACAGCTCACGGGAGCAAGAACGGTGCCTCGAGTCTTTATCGGTAAAGATTGTATA; GGCGGATGCAGTGATCTAGTCTCTTTGCAACAGAGTGGGGAACTGCTGACGCGGCTAAAG; CAGATTGGAGCTCTGCAGTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002064 unedited; TCACCATTTTGTAATACGACTTACTATTANNGGCGGCCGCGCAATTCGGCACGAGGGCAA; TACCTGCAACTGAGGATTCTTCCCGGGGAGACCGCAGCCCATCGGCATGGCTCAAGAGTT; TGTGAACTGCAAAATCCAGCCTGGGAAGGTGGTTGTGTTCATCAAGCCCACCTGCCCGTA; CTGCAGGAGGGCCCAAGAGATCCTCAGTCAATTGCCCATCAAACAAGGGCTTCTGGAATT; TGTCGATATCACAGCCACCAACCACACTAACGAGATTCAAGATTATTTGCAACAGCTCAC; GGGAGCAAGAACGGTGCCTCGAGTCTTTATCGGTAAAGATTGTATAGGCGGATGCAGTGA; TCTAGTCTCTTTGCAACAGAGTGGGGAACTGCTGACGCGGCTAAAGCAGATTGGAGCTCT; GCAGTAACCACAGGTGAGTGGCAGGTGAGGTGCTGGGCCAGGAACATGGGAGACCGTGGA; ATTCTTCTGTCGTGGTTTGTTATACTGTATGACAGCATTCTGGTTGGGGGAAGAAATGAT; TTGGGGTGAGTTGAGGAACGGTGATGTCAATCACTTATGAATTTCACACTTTACANAGAG; ATCGTGTCAATGAGAGAAATCCTTTCACTCAAGCTTCATTTAAGAAATATTTATTAAGCA; TGTGCCAGACACATGAGAATATAATGATGCATAAAATGATAACCCTGGACTTATATTTTA; GCAGAGTAGACCGATCAAAAACAAGCAAATTCTGCAATAGAATAGAATAATGTGACAGAA; TATGGCATGGGGTGGCGGGGAAAATTGAGCTAACATGGTAAAGGGAGGCCCCTTCCTCAC; GAAAGGGTTGAGCTTGACACCTGGTTTACCAAGGGGACTGGCTTTCCAAACATGAAGCAC; AAGAGCTTTTTCAGCCATAGGGAATAACCAGTGCGAAAGCTTCTGAGCTGCAACAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002064 unedited; CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATCTATAGGTTTTTTTTA; TTAAAAAAAAAATTAAGTGTTGTCACTAGAGATTGAAGGAAATTAACAATCTTACAATTC; TAGAACAGGCAAACTTCATGATAAGGGATTCTTAAGTTTTCAAAGTGTAGGAGCTCTTTC; ACAGATACACTCTTGGTGTAGGGGGCTGTAAGTTTTATCAGATGTTTTACATTTCATGCT; TTAATCTTTGCTGGTAGTCTAAATAAAATAAATACACAGTGTTCAGGATTATCTGGATAG; CCATCTTAAATAACAGAGTGTTCCGCATGACCACAGCCTCTGCACTTGTGCCTCTGATCC; ATATGACACCAAGACCAACTATGCTTTTCATATGATTTCTCCAATTGGGTCCTGTGACCT; TTCACAGAATTGTTGAACATTTCCTATGAGATCTGTGGAGGACAAGGTGGAGAACACTGG; TTCAGAGCAGTGATTTTCAAGGTGGGATGGGATGGGGTGGAGTTGAGTGGAGAGATGCAT; GTATTTACAAAAGACACATTCAAAATACCCACTGATTTCATAATACAAACTCCCTAAAGC; CCAACAGCAAAATGATTTTAGCTAATGGGCCAGAGCAAATAACCAACTGTGGACTCATCA; TTGTGGAAAAAGGACCCTGGATAAACCATATTGAAAAACCCTGTCCTAAAGGTAGAAACC; TGCCGACCCTGGCAGAACAGTAGAAACCCCTGCCCAAGAAGAACATTGGAGGTCACGGAG; CCGATCCTGTGCCAACACTGTCCAGTCTTTTGGAAACCAAGTCCCCTCCCCAATAACACC; AAGTCGGCCCCCCACCTTTTGCCCCTCGCAAAAAAAAACCCGCCCCCTTTTAAACCTGTC; CAACAAGGAAAACGCCGTTTGCCCCCTATTCTTCTCCCAAAGGTCTTTCCTTTCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_002064
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002064.1, NP_002055.1
• RefSeq Size: 1328 bp
• RefSeq ORF: 321 bp
• Locus ID: 2745
• UniProt ID: P35754
• Cytogenetics: 5q15
• Domains: glutaredoxin
• Protein Families: Druggable Genome
• Summary: This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Aug 2011]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1-4 encode the same protein.