PARN (NM_002582) Human Untagged Clone

SKU SC118566

PARN (untagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PARN
• Synonyms: DAN; DKCB6; PFBMFT4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002582, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAGATAATCAGGAGCAATTTTAAGAGTAATCTTCACAAAGTGTACCAGGCCATAGAGGAGGCCGACT; TCTTCGCCATCGATGGGGAGTTTTCAGGAATCAGTGATGGACCTTCAGTCTCTGCATTAACAAATGGTTT; TGACACTCCAGAAGAGAGGTATCAGAAGCTTAAAAAGCATTCCATGGACTTTTTGCTATTTCAGTTTGGC; CTTTGCACTTTTAAGTATGACTACACAGATTCAAAGTATATAACGAAGTCATTTAACTTCTATGTTTTCC; CGAAACCCTTCAATAGATCCTCACCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCATTGACTTTCTAGC; AAGCCAGGGATTTGATTTTAATAAAGTTTTTCGAAATGGAATTCCATATTTAAATCAGGAAGAAGAAAGA; CAGTTAAGAGAGCAGTATGATGAAAAACGTTCACAGGCGAATGGTGCAGGAGCTCTGTCCTATGTATCTC; CTAACACTTCAAAATGTCCTGTCACGATTCCTGAGGATCAAAAGAAGTTTATTGACCAAGTGGTAGAGAA; AATAGAGGATTTATTACAAAGTGAAGAAAACAAGAACTTGGATTTAGAGCCATGTACCGGGTTCCAAAGA; AAACTAATTTATCAGACTTTGAGCTGGAAGTATCCGAAAGGCATTCATGTTGAGACTTTAGAAACTGAAA; AGAAGGAGCGATATATAGTTATCAGCAAAGTAGATGAAGAAGAACGCAAAAGAAGAGAGCAGCAGAAACA; TGCCAAAGAACAGGAGGAGCTGAATGATGCTGTGGGATTTTCTAGAGTCATTCACGCCATTGCTAATTCG; GGAAAACTTGTTATTGGACACAATATGCTCTTGGACGTCATGCACACAGTTCATCAGTTCTACTGCCCTC; TGCCTGCGGACTTAAGTGAGTTTAAAGAGATGACAACATGTGTTTTCCCCAGACTCTTGGATACTAAATT; GATGGCCAGCACACAACCTTTTAAGGATATCATTAACAACACATCCCTTGCGGAATTGGAAAAGCGGTTA; AAAGAGACACCTTTCAACCCTCCTAAAGTTGAAAGTGCCGAAGGTTTTCCAAGTTATGACACAGCCTCTG; AACAACTCCACGAGGCAGGCTACGATGCCTACATCACAGGGCTGTGCTTCATCTCCATGGCCAATTACCT; AGGTTCTTTTCTCAGCCCTCCAAAAATTCATGTGTCTGCCAGATCAAAACTCATTGAACCTTTTTTTAAC; AAGTTATTTCTTATGAGGGTCATGGATATCCCCTATCTAAACTTGGAAGGACCAGACTTGCAGCCTAAAC; GTGATCATGTTCTCCATGTGACATTCCCCAAAGAATGGAAAACCAGCGACCTTTACCAGCTTTTCAGTGC; CTTTGGTAACATTCAGATATCCTGGATTGATGACACATCAGCATTTGTTTCCCTTAGCCAGCCCGAGCAA; GTAAAGATTGCTGTCAATACCAGCAAATATGCAGAAAGCTATCGGATCCAAACCTATGCTGAATATATGG; GGAGAAAACAGGAAGAGAAGCAGATCAAAAGAAAGTGGACTGAAGATAGCTGGAAGGAGGCTGACAGCAA; ACGGTTAAACCCCCAGTGCATACCCTACACCCTGCAGAATCACTATTACCGCAACAATAGTTTTACAGCT; CCCAGCACAGTAGGAAAGAGAAATTTGAGTCCTAGTCAAGAGGAAGCTGGCCTGGAGGACGGAGTGTCAG; GGGAGATTTCCGACACTGAGCTTGAGCAGACCGATTCCTGTGCAGAGCCCCTCTCAGAGGGAAGGAAAAA; GGCCAAGAAATTAAAAAGAATGAAGAAGGAGCTTTCTCCAGCAGGAAGCATCTCGAAGAACAGCCCTGCC; ACACTCTTTGAAGTTCCTGACACATGGTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002582 unedited; CCTGTCGCATACGNATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGGGTGGGGC; CAAGGTTCCGGCCACTCTGCAGAATGGAGATAATCAGGAGCAATTTTAAGAGTAATCTTC; ACAAAGTGTACCAGGCCATAGAGGAGGCCGACTTCTTCGCCATCGATGGGGAGTTTTCAG; GAATCAGTGATGGACCTTCAGTCTCTGCATTAACAAATGGTTTTGACACTCCAGAAGAGA; GGTATCAGAAGCTTAAAAAGCATTCCATGGACTTTTTGCTATTTCAGTTTGGCCTTTGCA; CTTTTAAGTATGACTACACAGATTCAAAGTATATAACGAAGTCATTTAACTTCTATGTTT; TCCCGAAACCCTTCAATAGATCCTCACCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCA; TTGACTTTCTAGCAAGCCAGGGATTTGATTTTAATAAAGTTTTTCGAAATGGAATTCCAT; ATTTAAATCAGGAAGAAGAAAGACAGTTAAGAGAGCAGTATGATGAAAAACGTTCACAGG; CGAATGGTGCAGGAGCTCTGTCCTATGTATCTCCTAACACTTCAAAATGTCCTGTCACGA; TTCCTGAGGATCAAAAGAAGTTTATTGACCAAGTGGTAGAGAAAATAGAGGATTTATTAC; AAAGTGAAGAAAACAAGAACTTGGATTTAGAGCCATGTACCGGGTTCCAAAGAAAACTAA; TTTATCAGACTNTGAGCTACTAGTATCCGAAAGGCATTCATGTTGAGACTTTAGAAACTG; TAAAGAGGAGCGATATATAGTTATCAGCAAAGTAGATGAAGAAAGACGCCAAAGAAGAGA; GCAGCAGAAACATGCCAAAGAACAGGAGGAGCTGAATGATGCCTGNGGGATTTNCTAGAG; TCATTCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002582 unedited; CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTATGAAAATGTTTTTAA; TTTTCATCTTTTGGAAATACATTTTTCATTTTTATTTCCACCATACAAAAATGTGAAATA; TCTAACAATGATCTATCTGAAGCGGGTGGAGCAAAGCAGCGCCATGAGCGTTTGTCGTTG; CTGTGATCTGTTTCAACGGAGAATGGGCTGGGACATGTTGTAGATTTGCACGATTTCACA; CACACACACACACACACACACACACACACACACACACAGACACGTACGCACACACGCTGC; CGTACCCCGAGACCGCCATCCAAACAAACGAACAGAGACTCTGGAAAGTGAACACAGCGC; CACGCATAAGAACAGAAGTTAACCTTTTTACTCGTACATCCCCCATGAGAAACTCACGTC; TTAGGAGAAAGGAACTCTACATAAATATGCCCAAAGGCCAGGGCATACGGCAAGCCCTCT; CATGGGTGGGCATGAGTGGACATCTTCCTGAAGGAAGGACAAGCTTGAGAGCGTCATGTT; TTCAGGCAGCTATGGTGGGAAGCAGGGAGGCACAACCGATTTTTCCACTGGGAGACTAAC; CGAGGGCTGCAACTGCCTCAAAATCTCAGTGCGAGAAGATGCACAGGCAGGACAGTACAC; CTCCAAATTCATGATCACAGCAGCTGGAATGTCAGCTTTTTTTGCAGATTTCACAGCCGA; CACTCCCCATCAAGCAGGGTTCTTAAGCACACTGGGTCATGACAGGAAAGGCCTCACCAG; AGCAACACGGTATTCACTGGTTAGCCCGTACACATTCTTGACACCCTACACCGGGATGAG; TGTCATGTCACCAGCCGTTTAATTTAAAGGGGAAAAACCCGCCCCCAAATAAAACTGTTT; TTCCCCCCCAGGAACACTGGGTTCCACCCCTCCATACCACATTGATA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002582
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002582.1, NP_002573.1
• RefSeq Size: 2984 bp
• RefSeq ORF: 1920 bp
• Locus ID: 5073
• UniProt ID: O95453
• Cytogenetics: 16p13.12
• Domains: CAF1, R3H
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• Summary: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).