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PMM1 (NM_002676) Human Untagged Clone

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SKU
SC118505
PMM1 (untagged)-Human phosphomannomutase 1 (PMM1)
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PMM1
Synonyms PMM 1; PMMH-22; Sec53
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118505 sequence for NM_002676 edited (data generated by NextGen Sequencing)
ATGGCAGTCACCGCCCAGGCAGCCCGCAGGAAGGAGCGCGTCCTCTGCCTGTTTGACGTG
GACGGGACCCTCACGCCGGCTCGCCAGAAAATTGACCCTGAGGTGGCCGCCTTCCTGCAG
AAGCTACGAAGTAGAGTGCAGATCGGTGTGGTGGGCGGCTCTGACTACTGTAAGATCGCT
GAGCAGCTGGGTGACGGGGATGAAGTCATTGAGAAGTTTGATTATGTGTTTGCCGAGAAC
GGGACGGTGCAGTATAAGCACGGACGACTGCTCTCCAAGCAGACCATCCAGAACCACCTG
GGGGAGGAGCTGCTGCAGGACTTGATCAACTTCTGCCTCAGCTACATGGCCCTGCTCAGG
CTGCCCAAGAAGCGTGGAACCTTCATCGAGTTCCGGAATGGCATGCTGAACATCTCGCCC
ATCGGCCGGAGCTGCACCCTGGAGGAGAGGATCGAGTTCTCCGAACTGGACAAGAAAGAG
AAGATCCGGGAGAAGTTCGTGGAAGCCCTGAAAACAGAGTTTGCTGGCAAAGGGCTGAGG
TTCTCTCGAGGAGGCATGATCAGCTTTGACGTCTTCCCCGAGGGCTGGGACAAGCGCTAC
TGCCTGGATAGCCTGGACCAGGACAGCTTCGACACCATCCACTTCTTTGGGAACGAGACT
AGCCCTGGTGGGAACGACTTTGAGATCTTTGCCGACCCCCGGACTGTTGGCCACAGCGTG
GTGTCTCCTCAGGACACGGTGCAGCGATGCCGGGAGATTTTCTTCCCAGAGACAGCTCAT
GAGGCGTGA

Clone variation with respect to NM_002676.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002676 unedited
CTCTGCATATTTGTCAATACGTACTCCACTATCAGGGGCGCGCCCGCGCAATTCGGCACG
AGGGCGGTACCTGCAGCCATGGTAGTCACCGCCCAGGCAGCCCGCAGGAAGGAGCGCGTC
CTCTGCCTGTTTGACGTGGACGGGACCCTCACGCCGGCTCGCCAGAAAATTGACCCTGAG
GTGGCCGCCTTCCTGCAGAAGCTACGAAGTAGAGTGCAGATCGGTGTGGTGGGCGGCTCT
GACTACTGTAAGATCGCTGAGCAGCTGGGTGACGGGGATGAAGTCATTGAGAAGTTTGAT
TATGTGTTTGCCGAGAACGGGACGGTGCAGTATAAGCACGGACGACTGCTCTCCAAGCAG
ACCATCCAGAACCACCTGGGGGACGAGCTGCTGCAGGACTTGATCAACTCCTGCCTCAAT
TACATGGCCCTGTTAAGGCTGCCAAAAAACGCGGAACCTTCATCGAGTCCCGAAAGGCTG
GCTTAAAATTTTCCCTACGGGCGGGGTTGCCCCTGGGGGAAAAGGATCAAGTTTTCCAAC
CGGCCAAAAAAAAAAAACCCGGCAAAAATCGGGAAACCCTAAAAAAAAAATTTTCGCGAA
AGGGCGGGGCTTTTCCCGGGGGGCATAAACCCCTTCTACCCCCCCCGGGAGGGGCGAAAA
CCCTCTTTTGTGTAATATGCCAACAAAAAAATCTCCACCCCCTCCCTCTTTGTGGAAAAA
ACACCCCCTGGGGAGACAAACTTAATATTTTCCCCCCCCCGCCGTGTGGACACCGGGGGT
TTTTCTCCTCCAATGCCCCCCCCGGGAACTCTCTTCCCTAAAACCACCCAGGAGGGGGCG
CCCCCCCCGTGGCCGCCCCTTTCAAAAACTCCCCCCCAAAACCGGGGGTGCGCAAACCGG
CCCCCCTCTCCCGTGACGTCCCCCCACCCCAGCGAGCGGGGCCCGCN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002676 unedited
CTCTCTTTTTTCTTTTTTCTTTTCCTTTGTTCCTGGGAAACTTTAATACTGTGACAAAGT
TCTCTAAAATAGGCACCTTCCCCACCGTACCTCATCGCCCAGGGCAGGCAGGCAGGGCAG
GCTAAATCTCGTACCGATACTTGAGCACGCCTCCTCCTGGTGCAAAAAGAAACCTCTTCT
GTACCGAAATACAAGCAGCAGCTGTGGCCTGGGCCACCAGGTGGAGCATGGGGAACACTC
TGGGCCCTGGGAGGACGAAGCCAGTGCCACTAGGAGCAGACTGGCTGGGGACGGTTGTCC
ACACAGACTCTGGCCCCATCTGGGTGGGCTTGCAGCAGGCGTCCTGGGCCAGAGGAGGGG
CCCTGGCATCTATCCAACACCAGGACCTCTCTTTAGGCCTAGGCCAAACTCTTCAGAAGT
CACGACACACAGATGTGGGCCCCGGTCACGCCTCATGAGCTGTCTCTGGGAAGAAAATCT
CCCGGCATCGCTGCACCGTGTCCTGAGGAGACACCACGCTGTGGCCAACAGTCCGGGGGT
CGGCAAAGATCTCAAAGTCGTTCCCACCAGGGCTAGTCTCGTTCCCAAAGAAGTGGATGG
TGTCGAAGCTGTCCTGGTCCAGGCTATCCAGGCAGTAGCGCTTGTCCCAGCCCTCGGGGA
AGACGTCAAAGCTGATCATGCCTCCTCGAGAAACCTCAGCCCTTTGCCAGCAAACTCTGT
TTTCAGGGCTTCCACGAACTTCTCCCGGATCTTCTCTTTCTTGTCCAGTTCGGAGAACTC
GATCCTCTCCTCCAGGTGCANCTCCGGCCGATGGNCGAGATGTTCANCATGCCCTTCCCG
GACTCGATGAAAGT
Restriction Sites NotI-NotI |
ACCN NM_002676
Insert Size 1350 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002676.1, NP_002667.1
RefSeq Size 1210 bp
RefSeq ORF 789 bp
Locus ID 5372
UniProt ID Q92871
Cytogenetics 22q13.2
Domains PMM
Protein Pathways Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
Summary Phosphomannomutase catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002676" in other vectors (5)
SKU Description Size Price
RC202004 PMM1 (Myc-DDK-tagged)-Human phosphomannomutase 1 (PMM1) 10 ug
$300.00
RC202004L3 Lenti ORF clone of Human phosphomannomutase 1 (PMM1), Myc-DDK-tagged 10 ug
$600.00
RC202004L4 Lenti ORF clone of Human phosphomannomutase 1 (PMM1), mGFP tagged 10 ug
$600.00
RG202004 PMM1 (tGFP-tagged) - Human phosphomannomutase 1 (PMM1) 10 ug
$489.00 $500.00
SC322342 PMM1 (untagged)-Human phosphomannomutase 1 (PMM1) 10 ug
$300.00

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