MEK6 (MAP2K6) (NM_002758) Human Untagged Clone

SKU SC118432

MAP2K6 (untagged)-Human mitogen-activated protein kinase kinase 6 (MAP2K6)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MEK6
• Synonyms: MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3; SAPKK3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002758 edited; ATGTCTCAGTCGAAAGGCAAGAAGCGAAACCCTGGCCTTAAAATTCCAAAAGAAGCATTT; GAACAACCTCAGACCAGTTCCACACCACCTAGAGATTTAGACTCCAAGGCTTGCATTTCT; ATTGGAAATCAGAACTTTGAGGTGAAGGCAGATGACCTGGAGCCTATAATGGAACTGGGA; CGAGGTGCGTACGGGGTGGTGGAGAAGATGCGGCACGTGCCCAGCGGGCAGATCATGGCA; GTGAAGCGGATCCGAGCCACAGTAAATAGCCAGGAACAGAAACGGCTACTGATGGATTTG; GATATTTCCATGAGGACGGTGGACTGTCCATTCACTGTCACCTTTTATGGCGCACTGTTT; CGGGAGGGTGATGTGTGGATCTGCATGGAGCTCATGGATACATCACTAGATAAATTCTAC; AAACAAGTTATTGATAAAGGCCAGACAATTCCAGAGGACATCTTAGGGAAAATAGCAGTT; TCTATTGTAAAAGCATTAGAACATTTACATAGTAAGCTGTCTGTCATTCACAGAGACGTC; AAGCCTTCTAATGTACTCATCAATGCTCTCGGTCAAGTGAAGATGTGCGATTTTGGAATC; AGTGGCTACTTGGTGGACTCTGTTGCTAAAACAATTGATGCAGGTTGCAAACCATACATG; GCCCCTGAAAGAATAAACCCAGAGCTCAACCAGAAGGGATACAGTGTGAAGTCTGACATT; TGGAGTCTGGGCATCACGATGATTGAGTTGGCCATCCTTCGATTTCCCTATGATTCATGG; GGAACTCCATTTCAGCAGCTCAAACAGGTGGTAGAGGAGCCATCGCCACAACTCCCAGCA; GACAAGTTCTCTGCAGAGTTTGTTGACTTTACCTCACAGTGCTTAAAGAAGAATTCCAAA; GAACGGCCTACATACCCAGAGCTAATGCAACATCCATTTTTCACCCTACATGAATCCAAA; GGAACAGATGTGGCATCTTTTGTAAAACTGATTCTTGGAGACTAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002758 unedited; GGTTGCACATTTTGTATACGACTCACTCATAGGGCGGCCGCGATTCGGCACGAGGTGTAG; CTGCAGCACAGCCTTCCCTAACGTTGCAACTGGGGNGAAAAATCACTTTCCAGTCTGTTT; TGCAAGGTGTGCATTTCCATCTTGATTCCCTGAAAGTCCATCTGCTGCATCGGTCAAGAG; AAACTCCACTTGCATGAAGATTGCACGCCTGCAGCTTGCATCTTTGTTGCAAAACTAGCT; ACAGAAGAGAAGCAAGGCAAAGTCTTTTGTGCTCCCCTCCCCCATCAAAGGAAAGGGGAA; AATGTCTCAGTCGAAAGGCAAGAAGCGAAACCCTGGCCTTAAAATTCCAAAAGAAGCATT; TGAACAACCTCAGACCAGTTCCACACCACCTAGAGATTTAGACTCCAAGGCTTGCATTTC; TATTGGAAATCAGAACTTTGAGGTGAAGGCAGATGACCTGGAGCCTATAATGGAACTGGG; ACGAGGTGCGTACGGGGTGGTGGAGAAGATGCGGCACGTGCCCAGCGGGCAGATCATGGC; AGTGAAGCGGATCCGAGCCACAGTAAATAGCCAGGAACAGAAACGGCTACTGATGGATTT; GGATATTTCCATGAGGACGGTGGACTGTCCATTCACTGTCACCTTTTATGGCGCACTGTT; TCGGGAGGGTGATGTGTGGATCTGCATGGAGCTCATGGATACATCACTAGATAAATTCTA; CAAACAAGTTATTGATAAAGGCCACGACATTCCAGAGGACATCTTAGGNAAAAATAGCAG; TTCTATTGTAAAAGCATTAGAACATTTACATAGTAAGCTGTCTGTCATTCACAGAGACGT; CAAGCCTTCTTATGTACTCATCAATGCTCTCCGGTCAGTGAAGATGTGCGATTNTGGAAT; CAGTGGCTCCTTGTGGGACCTGTTGCTAAAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_002758
• Insert Size: 1850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002758.2, NP_002749.2
• RefSeq Size: 2924 bp
• RefSeq ORF: 1005 bp
• Locus ID: 5608
• UniProt ID: P52564
• Cytogenetics: 17q24.3
• Domains: pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway
• Summary: This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) differs from variant 2 in its 5' terminus which contains an upstream in-frame translational start codon. Thus, this variant encodes isoform 1 that is 56 aa longer at N-terminus than isoform 2.