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HTRA1 (NM_002775) Human Untagged Clone

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SKU
SC118403
HTRA1 (untagged)-Human HtrA serine peptidase 1 (HTRA1)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol HTRA1
Synonyms ARMD7; CADASIL2; CARASIL; HtrA; L56; ORF480; PRSS11
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_002775 edited
GAATTCGGCACGAGGCTCCGGCCCTCGCCCTGTCCGCCGCCACCGCCGCCGCCGCCAGAG
TCGCCATGCAGATCCCGCGCGCCGCTCTTCTCCCGCTGCTGCTGCTGCTGCTGGCGGCGC
CCGCCTCGGCGCAGCTGTCCCGGGCCGGCCGCTCGGCGCCTTTGGCTGCCGGTTGCCCAG
ACCGCTGCGAGCCGGCGCGCTGCCCGCCGCAGCCGGAGCACTGCGAGGGCGGCCGGGCCC
GGGACGCGTGCGGCTGCTGCGAGGTGTGCGGCGCGCCCGAGGGCGCCGCGTGCGGCCTGC
AGGAGGGCCCGTGCGGCGAGGGGCTGCAGTGCGTGGTGCCCTTCGGGGTGCCAGCCTCGG
CCACGGTGCGGCGGCGCGCGCAGGCCGGCCTCTGTGTGTGCGCCAGCAGCGAGCCGGTGT
GCGGCAGCGACGCCAACACCTACGCCAACCTGTGCCAGCTGCGCGCCGCCAGCCGCCGCT
CCGAGAGGCTGCACCGGCCGCCGGTCATCGTCCTGCAGCGCGGAGCCTGCGGCCAAGGGC
AGGAAGATCCCAACAGTTTGCGCCATAAATATAACTTTATCGCGGACGTGGTGGAGAAGA
TCGCCCCTGCCGTGGTTCATATCGAATTGTTTCGCAAGCTTCCGTTTTCTAAACGAGAGG
TGCCGGTGGCTAGTGGGTCTGGGTTTATTGTGTCGGAAGATGGACTGATCGTGACAAATG
CCCACGTGGTGACCAACAAGCACCGGGTCAAAGTTGAGCTGAAGAACGGTGCCACTTACG
AAGCCAAAATCAAGGATGTGGATGAGAAAGCAGACATCGCACTCATCAAAATTGACCACC
AGGGCAAGCTGCCTGTCCTGCTGCTTGGCCGCTCCTCAGAGCTGCGGCCGGGAGAGTTCG
TGGTCGCCATCGGAAGCCCGTTTTCCCTTCAAAACACAGTCACCACCGGGATCGTGAGCA
CCACCCAGCGAGGCGGCAAAGAGCTGGGGCTCCGCAACTCAGACATGGACTACATCCAGA
CCGACGCCATCATCAACTATGGAAACTCGGGAGGCCCGTTAGTAAACCTGGACGGTGAAG
TGATTGGAATTAACACTTTGAAAGTGACAGCTGGAATCTCCTTTGCAATCCCATCTGATA
AGATTAAAAAGTTCCTCACGGAGTCCCATGACCGACAGGCCAAAGGAAAAGCCATCACCA
AGAAGAAGTATATTGGTATCCGAATGATGTCACTCACGTCCAGCAAAGCCAAAGAGCTGA
AGGACCGGCACCGGGACTTCCCAGACGTGATCTCAGGAGCGTATATAATTGAAGTAATTC
CTGATACCCCAGCAGAAGCTGGTGGTCTCAAGGAAAACGACGTCATAATCAGCATCAATG
GACAGTCCGTGGTCTCCGCCAATGATGTCAGCGACGTCATTAAAAGGGAAAGCACCCTGA
ACATGGTGGTCCGCAGGGGTAATGAAGATATCATGATCACAGTGATTCCCGAAGAAATTG
ACCCATAGGCAGAGGCATGAGCTGGACTTCATGTTTCCCTCAAAGACTCTCCCGTGGATG
ACGGATGAGGACTCTGGGCTGCTGGAATAGGACACTCAAGACTTTTGACTGCCATTTTGT
TTGTTCAGTGGAGACTCCCTGGCCAACAGAATCCTTCTTGATAGTTTGCAGGCAAAACAA
ATGTAATGTTGCAGATCCGCAGGCAGAAGCTCTGCCCTTCTGTATCCTATGTATGCAGTG
TGCTTTTTCTTGCCAGCTTGGGCCATTCTTGCTTAGACAGTCAGCATTTGTCTCCTCCTT
TAACTGAGTCATCATCTTAGTCCAACTAATGCAGTCGATACAATGCGTAGATAGAAGAAG
CCCCACGGGAGCCAGGATGGGACTGGTCGTGTTTGTGCTTTTCTCCAAGTCAGCACCCAA
AGGTCAATGCACAGAGACCCCGGGTGGGTGAGCGCTGGCTTCTCAAACGGCCGAAGTTGC
CTCTTTTAGGAATCTCTTTGGAATTGGGAGCACGATGACTCTGAGTTTGAGCTATTAAAG
TACTTCTTACACATTGAAAAAAAAAAAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002775 unedited
CAGATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATCGGCACGAGGCTCCGGCCC
TCGCCCTGTCCGCCGCCACCGCCGCCGCCGCCAGAGTCGCCATGCAGATCCCGCGCGCCG
CTCTTCTCCCGCTGCTGCTGCTGCTGCTGGCGGCGCCCGCCTCGGCGCAGCTGTCCCGGG
CCGGCCGCTCGGCGCCTTTGGCTGCCGGTTGCCCAGACCGCTGCGAGCCGGCGCGCTGCC
CGCCGCAGCCGGAGCACTGCGAGGGCGGCCGGGCCCGGGACGCGTGCGGCTGCTGCGAGG
TGTGCGGCGCGCCCGAGGGCGCCGCGTGCGGCCTGCAGGAGGGCCCGTGCGGCGAGGGGC
TGCAGTGCGTGGTGCCCTTCGGGGTGCCAGCCTCGGCCACGGTGCGGCGGCGCGCGCAGG
CCGGCCTCTGTGTGTGCGCCAGCAGCGAGCCGGTGTGCGGCAGCGACGCCAACACCTACG
CCAACCTGTGCCAGCTGCGCGCCGCCAGCCGCCGCTCCGAGAGGCTGCACCGGCCGCCGG
TCATCGTCCTGCAGCGCGGAGCCTGCGGCCAAGGGCAGGAAGATCCCAACAGTTTGCGCC
ATAAATATAACTTTATCGCGGACGTGGTGGAGAAGATCGCCCCTGCCGTGGTTCATATCG
AATTGTTTCGCAAGCTTCCGTTTTCTAAACGAGAGGTGCCGGTGGCTAGTGGGTCTGGGT
TTATTGTGTCGGAAGATGGACTGATCGTGACANATGCCCACGTGGTGACCAACAAGCACC
GGGTCAAAAGTGAGCTGAAGAACGGTGCCACTTACGAAGCCAAATCAAGGATGGGGATGA
GAAAGCAGACATCGCACTCATCAAAATTGACCACCCGGGGCAGCCTGCCTGTCCTGCTGC
TTGGCCGCTCCTCAGAGCTGCGCCGGGAAGAATTCGTGTCGCCATCGGAAGACCCTT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002775 unedited
CTTTGGACCGGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTAAATGTGTAA
GAAGTACTTTAATAGCTCAAACTCAGAGTCATCGTGCTCCCAATTCCAAAAAGATTCCTA
AAAGAGGCAACTTCGGCCGTTTGAGAAGCCAGCGCTCACCCACCCGGGGTCTCTGTGCAT
TGACCTTTGGGTGCTGACTTGGAGAAAAGCACAAACACGACCAGTCCCATCCTGGCTCCC
GTGGGGCTTCTTCTATCTACGCATTGTATCGACTGCATTAGTTGGACTAAGATGATGACT
CAGTTAAAGGAGGAGACAAATGCTGACTGTCTAAGCAAGAATGGCCCAAGCTGGCAAGAA
AAAGCACACTGCATACATAGGATACAGAAGGGCAGAGCTTCTGCCTGCGGATCTGCAACA
TTACATTTGTTTTGCCTGCAAACTATCAAGAAGGATTCTGTTGGCCAGGGAGTCTCCACT
GAACAAACAAAATGGCAGTCAAAAGTCTTGAGTGTCCTATTCCAGCAGCCCAGAGTCCTC
ATCCGTCATCCACGGAAGAGTCTTTGAGGGAAACATGAAGTCCAGCTCATGCCTCTGCCT
ATGGGTCAATTTCTTCGGGAATCACTGGGATCATGATATCTTTATTACCCCCTGCGACCA
CCATGTTCAGGGGTGCTTTCCCTTTTTAATGACGTCGCTTGACATTCATTGCCGGAAGAC
CACGGGACTGTTCCATTGGATGCCTGATTATGACCGTCGTTTTCCCTTGGAAACCCCCAC
TTTTTGCTTGGGGTATCAGGAATTTACTTCAATAATTTACCGCTTCCTGGAGATCCCGNT
CGGGGAAAGTCCCCGTTGGCCCGGCCCTTTCAGCTTTTTTGCTTTTGCTTGGACCGGAAG
GGACTTCATTTGGGATACCAAAAAACCTTTTTTTTGGGGAAAGCTTTTCCCTTGGCGCCG
TGCGG
Restriction Sites NotI-NotI |
ACCN NM_002775
Insert Size 2210 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002775.3, NP_002766.1
RefSeq Size 2133 bp
RefSeq ORF 1443 bp
Locus ID 5654
UniProt ID Q92743
Cytogenetics 10q26.13
Domains IB, kazal, PDZ, Tryp_SPc
Protein Families Druggable Genome, Protease, Secreted Protein
Summary This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002775" in other vectors (6)
SKU Description Size Price
RC222362 HTRA1 (Myc-DDK-tagged)-Human HtrA serine peptidase 1 (HTRA1) 10 ug
$457.00
RC222362L1 Lenti ORF clone of Human HtrA serine peptidase 1 (HTRA1), Myc-DDK-tagged 10 ug
$757.00
RC222362L2 Lenti ORF clone of Human HtrA serine peptidase 1 (HTRA1), mGFP tagged 10 ug
$757.00
RC222362L3 Lenti ORF clone of Human HtrA serine peptidase 1 (HTRA1), Myc-DDK-tagged 10 ug
$757.00
RC222362L4 Lenti ORF clone of Human HtrA serine peptidase 1 (HTRA1), mGFP tagged 10 ug
$757.00
RG222362 HTRA1 (tGFP-tagged) - Human HtrA serine peptidase 1 (HTRA1) 10 ug
$489.00 $657.00

file-alt Citations

The serine protease HtrA1 cleaves misfolded transforming growth factor β-induced protein (TGFBIp) and induces amyloid formation
Poulsen, Nielsen, Scavenius et al
J. Biol. Chem. (2019) 294 (31), 11817-11828 DOI: 10.1074/jbc.RA119.009050
AI Summary: OriGene contributed to this study by providing the HtrA1 cDNA (NM_002775) in the pCMV6-XL4 vector, which was used for recombinant expression and purification of the HtrA1 protein to investigate its role in TGFBIp cleavage and amyloid formation.
Snippet: storage at -80 ˚C. Recombinant expression and purification of HtrA1 HtrA1 cDNA inserted into the pCMV6-XL4 vector using NotI-NotI restriction sites was obtained from Origene (NM_002775). The translational and secreted
Protoporphyrins enhance oligomerization and enzymatic activity of HtrA1 serine protease
Jo, Patterson, Stoessel et al
PLoS One (2014) 9 (12), e115362 DOI: 10.1371/journal.pone.0115362
Cited by 7
AI Summary: In this study, OriGene’s HtrA1 shRNA lentiviral particles (TL313337V) and HtrA1 cDNA clone (RC209965) were used to manipulate HtrA1 expression in cell-based assays, enabling the authors to investigate the effects of protoporphyrins on HtrA1 oligomerization and enzymatic activity.
FigureFigureFigure
Showing 1-3 of 3 papers.
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