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PSMD9 (NM_002813) Human Untagged Clone

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SKU
SC118384
PSMD9 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 (PSMD9)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PSMD9
Synonyms p27; Rpn4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118384 sequence for NM_002813 edited (data generated by NextGen Sequencing)
ATGTCCGACGAGGAAGCGAGGCAGAGCGGAGGCTCCTCGCAGGCCGGCGMCGTGACTGTC
AGCGACGTCCAGGAGCTGATGCGGCGCAAGGAGGAGATAGAAGCGCAGATCAAGGCCAAC
TATGACGTGCTGGAAAGCCAAAAAGGCATTGGGATGAACGAGCCGCTGGTGGACTGTGAG
GGCTACCCCCGGTCAGACGTGGACCTGTACCAAGTCCGCACCGCCAGGCACAACATCATA
TGCCTGCAGAATGATCACAAGGCAGTGATGAAGCAGGTGGAGGAGGCCCTGCACCAGCTG
CACGCTCGCGACAAGGAGAAGCAGGCCCGGGACATGGCTGAGGCCCACAAAGAGGCCATG
AGCCGCAAACTGGGTCAGAGTGAGAGCCAGGGCCCTCCACGGGCCTTCGCCAAAGTGAAC
AGCATCAGCCCCGGCTCCCCAGCCAGCATCGCGGGTCTGCAAGTGGATGATGAGATTGTG
GAGTTCGGCTCTGTGAACACCCAGAACTTCCAGTCACTGCATAACATTGGCAGTGTGGTG
CAGCACAGTGAGGGGAAGCCCCTGAATGTGACAGTGATCCGCAGGGGGGAAAAACACCAG
CTTAGACTTGTTCCAACACGCTGGGCAGGAAAAGGACTGCTGGGCTGCAACATTATTCCT
CTGCAAAGATGA

Clone variation with respect to NM_002813.4
50 t=>m
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002813 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCCCTAGCCCG
GGAGCCGGGTCTCTGGAGTCGCGGCCCGGGGTTCACGATGTCCGACGAGGAAGCGAGGCA
GAGCGGAGGCTCCTCGCAGGCCGGCGCCGTGACTGTCAGCGACGTCCAGGAGCTGATGCG
GCGCAAGGAGGAGATAGAAGCGCAGATCAAGGCCAACTATGACGTGCTGGAAAGCCAAAA
AGGCATTGGGATGAACGAGCCGCTGGTGGACTGTGAGGGCTACCCCCGGTCAGACGTGGA
CCTGTACCAAGTCCGCACCGCCAGGCACAACATCATATGCCTGCAGAATGATCACAAGGC
AGTGATGAAGCAGGTGGAGGAGGCCCTGCACCAGCTGCACGCTCGCGACAAGGAGAAGCA
GGCCCGGGACATGGCTGAGGCCCACAAAGAGGCCATGAGCCGCAAACTGGGTCAGAGTGA
GAGCCAGGGCCCTCCACGGGCCTTCGCCAAAGTGAACAGCATCAGCCCCGGCTCCCCAGC
CAGCATCGCGGGTCTGCAAGTGGATGATGAGATTGTGGAGTTCGGCTCTGTGAACACCCA
GAACTTCCAGTCACTGCATAACATTGGCAGTGTGGTGCAGCACAGTGAGGGGAAGCCCCT
GAATGTGACAGTGATCCGCAGGGGGGAAAAACACCAGCTTAGACTTGTTCCCACACGCTG
GGCANGAAAAGGACTGCTGGGCTGCAACATTATTCCTCTGCAAGATGATTGTCCCTGGGG
AAACAGTACAGGNAAGCATCTTTCCCTTGCCCTGGACTNGGGTCTAGGGGATTTCCACTT
GTCNTCTCTNCCCTGAGCAATAAGATCTGGNAAGAGGCCTTGTACCTGAACCTCTGN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002813 unedited
CCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACATATCAGCACTGTAAA
ACTCTTTATTAAAAATAATAATAATAATCACACCAGTACCTGCCAGATAACTTCCGCCCA
CTTGCCCACAATCCAGGGAATTCCTATTTAGGGCCGTGGCCTAATTTGTGAAAGAGGCCA
AGCCTAAGTTTTTAAGAATGCCTTAATCCAGGGAGATTACACTGGTGGGCCACAGTACTG
CCACCACACAGAAGTTCAGGTTACAAGCCTCTTCCAGATCCTTATGCTTCAGGGAGAGAA
GACAAGTTGGAAATCCCTAGACCCAAGTCCAGGGCAAGGGAAGATGCTTTCCTGTTACTG
TTCCCCAGGGACAATCATCTTTGCAGAGGAATAATGTTGCAGCCCAGCAGTCCTTTTCCT
GCCCAGCGTGTTGGAACAAGTCTAAGCTGGTGTTTTTCCCCCCTGCGGATCACTGTCACA
TTCAGGGGCTTCCCCTCACTGTGCTGCACCACACTGCCAATGTTATGCAGTGACTGGAAG
TTCTGGGTGTTCACAGAGCCGAACTCCACAATCTCATCATCCACTTGCANACCCGCGATG
CTGGCTGGGGAGCCGGGGCTGATGCTGTTCACTTTGGCGAAAGCCCGTGGAGGGCCCTGG
CTCTCACTTCTGACCCCATTTGCGGTTCATGGCCCTCTTTGTGGGCCTCAGCCATGTCCC
GGGGCCTGTTNTTTCTTGTCCCGAGCGTGCACCTGGTGCAAGGCCTTCTCCACCTGCCAA
ATAAATGCCTTGTGATCATTTTGCAGGCATATGAATGTTGGGCCTGGCGGGGCCGACTTG
GTACCATGTCCAACTTTTGACCGGGGGGTAGCCTTAACAACCAACACGGGGCTTGTTATT
TCCATGGCCTTTTTGCCTTTCCCACCGTCATATATGGCCTTGATCTGCGCTATTATT
Restriction Sites NotI-NotI |
ACCN NM_002813
Insert Size 1140 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002813.4, NP_002804.2
RefSeq Size 2360 bp
RefSeq ORF 672 bp
Locus ID 5715
UniProt ID O00233
Cytogenetics 12q24.31
Domains PDZ
Summary The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene. provided by RefSeq, May 2012
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_002813" in other vectors (6)
SKU Description Size Price
RC200903 PSMD9 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 (PSMD9) 10 ug
$300.00
RC200903L1 Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 (PSMD9), Myc-DDK-tagged 10 ug
$600.00
RC200903L2 Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 (PSMD9), mGFP tagged 10 ug
$600.00
RC200903L3 Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 (PSMD9), Myc-DDK-tagged 10 ug
$600.00
RC200903L4 Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 (PSMD9), mGFP tagged 10 ug
$600.00
RG200903 PSMD9 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 9 (PSMD9) 10 ug
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file-alt Citations

Discovery of novel interacting partners of PSMD9, a proteasomal chaperone: Role of an Atypical and versatile PDZ-domain motif interaction and identification of putative functional modules
Sangith, Srinivasaraghavan, Sahu et al
FEBS Open Bio (2014) 4, 571-83 DOI: 10.1016/j.fob.2014.05.005
Cited by 16
AI Summary: OriGene Technologies contributed to this study by providing the PSMD9 cDNA, which was used to express and analyze the protein's interactions with novel partners.
Snippet: 2. Material and methods 2.1. Plasmids PSMD9 cDNA (Origene Technologies) was amplified and ligated into pRSETA vector between BamHI and EcoRI sites. hnRNPA1 and S14 ribosomal protein cDNA was generated by RT-PCR from RNA extracted from HEK293 ... Cite SaveSaving
FigureFigureFigure
A novel role for the Proteasomal Chaperone PSMD9 and hnRNPA1 in enhancing IκBα degradation and NF-κB activation - Functional Relevance of predicted PDZ domain-motif interaction
Sahu, Sangith, Ramteke et al
FEBS J. (2014)
Cited by 38
AI Summary: OriGene’s PSMD9 cDNA was used as the template to amplify and clone PSMD9 for expression studies, enabling the authors to investigate PSMD9’s role in IκBα degradation and NF-κB activation.
Snippet: metabolism remains to be seen. Materials and Methods Plasmids PSMD9 was amplified from PSMD9 cDNA (Origene Technologies) and cloned within HindIII &EcoRI in p3xFLAG-CMV-10 mammalian expression vector (Sigma). For bacterial expression ... Cite SaveSaving
Showing 1-2 of 2 papers.
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