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PYGL (NM_002863) Human Untagged Clone

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SKU
SC118381
PYGL (untagged)-Human phosphorylase, glycogen, liver (PYGL), transcript variant 1
  $776.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PYGL
Synonyms GSD6
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118381 sequence for NM_002863 edited (data generated by NextGen Sequencing)
ATGGCGAAGCCCCTGACGGACCAGGAGAAGCGGCGGCAGATCAGCATCCGCGGCATCGTG
GGCGTGGAGAACGTGGCAGAGCTGAAGAAGAGTTTCAACCGGCACCTGCACTTCACGCTG
GTCAAGGACCGCAACGTGGCCACCACCCGCGACTACTACTTCGCGCTGGCGCACACGGTG
CGCGACCACCTGGTGGGGCGCTGGATCCGCACGCAGCAGCACTACTACGACAAGTGCCCC
AAGAGGGTATATTACCTCTCTCTGGAATTTTACATGGGCCGAACATTACAGAACACCATG
ATCAACCTCGGTCTGCAAAATGCCTGTGATGAGGCCATTTACCAGCTTGGATTGGATATA
GAAGAGTTAGAAGAAATTGAAGAAGATGCTGGACTTGGCAATGGTGGTCTTGGGAGACTT
GCTGCCTGCTTCTTGGATTCCATGGCAACCCTGGGACTTGCAGCCTATGGATACGGCATT
CGGTATGAATATGGGATTTTCAATCAGAAGATCCGAGATGGATGGCAGGTAGAAGAAGCA
GATGATTGGCTCAGATATGGAAACCCTTGGGAGAAGTCCCGCCCAGAATTCATGCTGCCT
GTGCACTTCTATGGAAAAGTAGAACACACCAACACCGGGACCAAGTGGATTGACACTCAA
GTGGTCCTGGCTCTGCCATATGACACCCCCGTGCCCGGCTACATGAATAACACTGTCAAC
ACCATGCGCCTCTGGTCTGCTCGGGCACCAAATGACTTTAACCTCAGAGACTTTAATGTT
GGAGACTACATTCAGGCTGTGCTGGACCGAAACCTGGCCGAGAACATCTCCCGGGTCCTC
TATCCCAATGACAATTTTTTTGAAGGGAAGGAGCTAAGATTGAAGCAGGAATACTTTGTG
GTGGCTGCAACCTTGCAAGATATCATCCGCCGTTTCAAAGCCTCCAAGTTTGGCTCCACC
CGTGGTGCAGGAACTGTGTTTGATGCCTTCCCGGATCAGGTGGCCATCCAGCTGAATGAT
ACTCACCCTGCACTCGCGATCCCTGAGCTGATGAGGATTTTTGTGGATATTGAAAAACTG
CCCTGGTCCAAGGCATGGGAGCTCACCCAGAAGACCTTCGCCTACACCAACCACACAGTG
CTCCCGGAAGCCCTGGAGCGCTGGCCCGTGGACCTGGTGGAGAAGCTGCTCCCTCGACAT
TTGGAAATCATTTATGAGATAAATCAGAAGCATTTAGATAGAATTGTGGCCTTGTTTCCT
AAAGATGTGGACCGTCTGAGAAGGATGTCTCTGATAGAAGAGGAAGGAAGCAAAAGGATC
AACATGGCCCATCTCTGCATTGTCGGTTCCCATGCTGTGAATGGCGTGGCTAAAATCCAC
TCAGACATCGTGAAGACTAAAGTATTCAAGGACTTCAGTGAGCTAGAACCTGACAAGTTT
CAGAATAAAACCAATGGGATCACTCCAAGGCGCTGGCTCCTACTCTGCAACCCAGGACTT
GCAGAGCTCATAGCAGAGAAAATTGGAGAAGACTATGTGAAAGACCTGAGCCAGCTGACG
AAGCTCCACAGCTTCCTGGGTGATGATGTCTTCCTCCGGGAACTCGCCAAGGTGAAGCAG
GAGAATAAGCTGAAGTTTTCTCAGTTCCTGGAGACGGAGTACAAAGTGAAGATCAACCCA
TCCTCCATGTTTGATGTCCAGGTGAAGAGGATACATGAGTACAAGCGACAGCTCTTGAAC
TGTCTGCATGTGATCACGATGTACAACCGCATTAAGAAAGACCCTAAGAAGTTATTCGTG
CCAAGGACAGTTATCATTGGTGGTAAAGCTGCCCCAGGATATCACATGGCCAAAATGATC
ATAAAGCTGATCACTTCAGTGGCAGATGTGGTGAACAATGACCCTATGGTTGGAAGCAAG
TTGAAAGTCATCTTCTTGGAGAACTACAGAGTATCTCTTGCTGAAAAAGTCATTCCAGCC
ACAGATCTGTCAGAGCAGATTTCCACTGCAGGCACCGAAGCCTCGGGGACAGGCAATATG
AAGTTCATGCTAAATGGGGCCCTAACTATCGGGACCATGGATGGGGCCAATGTGGAAATG
GCAGAAGAAGCTGGGGAAGAGAACCTGTTCATCTTTGGCATGAGGATAGATGATGTGGCT
GCTTTGGACAAGAAAGGGTACGAGGCAAAAGAATACTATGAGGCACTTCCAGAGCTGAAG
CTGGTCATTGATCAAATTGACAATGGCTTTTTTTCTCCCAAGCAGCCTGACCTCTTCAAA
GATATCATCAACATGCTATTTTATCATGACAGGTTTAAAGTCTTTGCAGACTACGAAGCC
TATGTCAAGTGTCAAGATAAAGTGAGTCAGCTGTACATGAATCCAAAGGCCTGGAACACA
ATGGTACTCAAAAACATAGCTGCCTCGGGGAAATTCTCCAGTGACCGAACAATTAAAGAA
TATGCCCAAAACATCTGGAACGTGGAACCTTCAGATCTAAAGATTTCTCTATCCAATGAA
TCTAACAAAGTCAATGGAAATTGA

Clone variation with respect to NM_002863.4
1020 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002863 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCAGCCCAGCGCCTCCG
GCCGCACTTCCAGCTCTCTGCGCAGCCCGCCGCGCAGCCCGCCGCCCCAGCCATGGCGAA
GCCCCTGACGGACCAGGAGAAGCGGCGGCAGATCAGCATCCGCGGCATCGTGGGCGTGGA
GAACGTGGCAGAGCTGAAGAAGAGTTTCAACCGGCACCTGCACTTCACGCTGGTCAAGGA
CCGCAACGTGGCCACCACCCGCGACTACTACTTCGCGCTGGCGCACACGGTGCGCGACCA
CCTGGTGGGGCGCTGGATCCGCACGCAGCAGCACTACTACGACAAGTGCCCCAAGAGGGT
ATATTACCTCTCTCTGGAATTTTACATGGGCCGAACATTACAGAACACCATGATCAACCT
CGGTCTGCAAAATGCCTGTGATGAGGCCATTTACCAGCTTGGATTGGATATAGAAGAGTT
AGAAGAAATTGAAGAAGATGCTGGACTTGGCAATGGTGGTCTTGGGAGACTTGCTGCCTG
CTTCTTGGATTCCATGGCAACCCTGNGACTTGCAGCCTATGGATACGGCATTCGGTATGA
ATATGGGATTTTCAATCAGAAGATCCGAGATGGATGGCAGGTAGAAGAAGCAGATGATTG
GCTCAGATATGGAAACCCTTGGGAGAAGTCCCGCCCAGAATTCATGCTGCCTGTGCACTT
CTNATGGAAAGTAGAACACACCAACACCGGACCAAGTGATTGACACTCAAGTGGTCCTGG
CTCTGCATATGACCCCCCGTGCCGGCTACATGATACACTGTCACACCTGCCCTCTGTCTG
CTCGGCACCAATGACTTTACCTANAACCTTATGTTGAGACTCATCCAGCTGGCTGACCGA
ACCTGCCGAGACACTNCCGGTCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002863 unedited
CTATGGACCGGCGGCCCGCATTCTNANGATNCGNAGTTTTCTTTCTTTTTTCTTTTTTCT
TTAGACCCTTGGAAAATTGACACTTTATTTTTAAGCTCTATTACATATAATTTCCCTCCC
CATTCCCAGAGATACTCAACTATTATAGATTATTAGCTAACAAAACAAAAACCAGTGAAT
GTTGTAAAAATGTTCAAGTTCAGTAAGAAGCTATGTTTTCTAGAGACATTGTAGAGTTCA
ATTTCCATTGACTTTGTTAGATTCATTGGATAGAGAAATCTTTAGATCTGAAGGTTCCAC
GTTCCAGATGTTTTGGGCATATTCTTTAATTGTTCGGTCACTGGAGAATTTCCCCGAGGC
AGCTATGTTTTTGAGTACCATTGTGTTCCAGGCCTTTGGATTCATGTACAGCTGACTCAC
TTTATCTTGACACTTGACATAGGCTTCGTAGTCTGCAAAGACTTTAAACCTGTCATGATA
AAATAGCATGTTGATGATATCTTTGAAGAGGTCAGGCTGCTTGGGAGAAAAAAAGCCATT
GTCAATTTGATCAATGACCAGCTTCAGCTCTGGAAGTGCCTCATAGTATTCTTTTGCCTC
GTACCCTTTCTTGTCCAAAGCAGCCACATCATCTATCCTCATGCCAAAGATGAACAGGTT
CTCTTCCCCAGCTTCTTCTGCCATTTCCACATTGGCCCCATCCATGGTCCCGATAGTTAG
GGCCCCATTTAGCATGAACTTCATATTGCCTGTCCCCGAGGCTTCGGTGCCTGCAGTGGA
AATCTGCTCTGACAGATCTGTGGCTGGAATGACCTTTTCAGCAAGAGAACTCTGTAGTTC
TCCAAGAGATGACTTTCAACTTGCTTCCACCATAGGGTCATTGNTACCACATCTGCCACT
GAGTGATAGCTTTAGATCATTTTGCCTGC
Restriction Sites NotI-NotI |
ACCN NM_002863
Insert Size 2950 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002863.3, NP_002854.3
RefSeq Size 2830 bp
RefSeq ORF 2544 bp
Locus ID 5836
UniProt ID P06737
Cytogenetics 14q22.1
Domains phosphorylase
Protein Families Druggable Genome
Protein Pathways Insulin signaling pathway, Starch and sucrose metabolism
Summary This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.provided by RefSeq, Feb 2011
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_002863" in other vectors (6)
SKU Description Size Price
RC210683 PYGL (Myc-DDK-tagged)-Human phosphorylase, glycogen, liver (PYGL), transcript variant 1 10 ug
$776.00
RC210683L1 Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, Myc-DDK-tagged 10 ug
$1,076.00
RC210683L2 Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, mGFP tagged 10 ug
$1,076.00
RC210683L3 Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, Myc-DDK-tagged 10 ug
$1,076.00
RC210683L4 Lenti ORF clone of Human phosphorylase, glycogen, liver (PYGL), transcript variant 1, mGFP tagged 10 ug
$1,076.00
RG210683 PYGL (tGFP-tagged) - Human phosphorylase, glycogen, liver (PYGL), transcript variant 1 10 ug
$976.00

file-alt Citations

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