RFC4 (NM_002916) Human Untagged Clone

SKU SC118346

RFC4 (untagged)-Human replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RFC4
• Synonyms: A1; RFC37
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118346 sequence for NM_002916 edited (data generated by NextGen Sequencing); ATGCAAGCATTTCTTAAAGGTACATCCATCAGTACTAAACCCCCGCTGACCAAGGATCGA; GGAGTAGCTGCCAGTGCGGGAAGTAGCGGAGAGAACAAGAAAGCCAAACCCGTTCCCTGG; GTGGAAAAATATCGCCCAAAATGTGTGGATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCA; GTGCTGAAAAAATCTTTAGAAGGAGCAGATCTTCCTAATCTCTTGTTTTACGGACCACCT; GGAACTGGAAAAACATCCACTATTTTGGCAGCAGCTAGAGAACTCTTTGGGCCTGAACTT; TTCCGATTAAGAGTTCTTGAGTTAAATGCATCTGATGAACGTGGAATACAAGTAGTTCGA; GAGAAAGTGAAAAATTTTGCTCAATTAACTGTGTCAGGAAGTCGCTCAGATGGGAAGCCG; TGTCCGCCTTTTAAGATTGTGATTCTGGATGAAGCAGATTCTATGACCTCAGCTGCTCAG; GCAGCTTTAAGACGTACCATGGAGAAGGAGTCGAAAACCACCCGATTCTGTCTTATCTGT; AACTATGTCAGTCGAATAATTGAACCCCTGACCTCTAGATGTTCAAAATTCCGCTTCAAG; CCTCTGTCAGATAAAATTCAACAGCAGCGATTACTAGACATTGCCAAGAAGGAAAATGTC; AAAATTAGTGATGAGGGAATAGCTTATCTTGTTAAAGTGTCAGAAGGAGACTTAAGAAAA; GCCATTACATTTCTTCAAAGCGCTACTCGATTAACAGGTGGAAAGGAGATCACAGAGAAA; GTGATTACAGACATTGCTGGGGTAATACCAGCTGAGAAAATTGATGGAGTATTTGCTGCC; TGTCAGAGTGGCTCTTTTGACAAACTAGAAGCTGTGGTCAAGGATTTAATAGATGAGGGT; CATGCAGCAACTCAGCTCGTCAATCAACTCCATGATGTGGTTGTAGAAAATAACTTATCT; GATAAACAGAAGTCTATTATCACAGAAAAACTTGCCGAAGTTGACAAATGCCTAGCAGAT; GGTGCTGATGAACATTTGCAACTCATCAGCCTTTGTGCAACTGTGATGCAGCAGTTATCT; CAGAATTGTTAA; ; Clone variation with respect to NM_002916.3; 798 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002916 unedited; TTTGTATACGACTCACTATGGGCGGCNCGCGAATTCGCACGAGGGACTGTGGGGACATCA; GTGATCGTAAGTCTCCTGGGCCCGTTATTCTCAGATTAGGTGACGGAGCTAAGACTTCGA; GACCATCTCGTCCTTTTTGTATCGCGGAAACCTGAGGAACGAGCCGGCGGCGGTGACCTG; CACGAGAAGCCAGGCTAACTGGGTGAAGTACCATGCAAGCATTTCTTAAAGGTACATCCA; TCAGTACTAAACCCCCGCTGACCAAGGATCGAGGAGTAGCTGCCAGTGCGGGAAGTAGCG; GAGAGAACAAGAAAGCCAAACCCGTTCCCTGGGTGGAAAAATATCGCCCAAAATGTGTGG; ATGAAGTTGCTTTCCAGGAAGAAGTGGTTGCAGTGCTGAAAAAATCTTTAGAAGGAGCAG; ATCTTCCTAATCTCTTGTTTTACGGACCACCTGGAACTGGAAAAACATCCACTATTTTGG; CAGCAGCTAGAGAACTCTTTGGGCCTGAACTTTTCCGATTAAGAGTTCTTGAGTTAAATG; CATCTGATGAACGTGGAATACAAGTAGTTCGAGAGAAAGTGAAAAATTTTGCTCAATTAA; CTGTGTCAGGAAGTCGCTCAGATGGGAAGCCGTGTCCGCCTTTTAAGATTGTGATTCTGG; ATGAAGCAGATTCTATGACCTCAGCTGCTCAGGCAGCTTTAAGACGTACCATGGAGAAGG; AGTCGAAAACCACCCCGATCTGTCTTATCTGTAACTATGTCAGTCGAATAATTGAACCCC; TGACCTCTAGATGTTCAAAATTCCGCCTTCAGGCTCTGTCAGATAAATTCAACAGCAGCG; ATTACTAGACATTGCCCAGAAGGAAATGTCAAAATAGGATGAGGGATAGCTATCTTGGTT; AGGGTCGAGAGACTTAGAAAGCCTTCATTCTTCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002916 unedited; TTTGGACGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTGGGCATTTTATTTTTATTACAACTTCATTATTTACAAAACCCCCCATCCAGA; TATATTCACGTTAACAATTCTGAGATAACTGCTGCATCACAGTTGCACAAAGGCTGATGA; GTTGCAAATGTTCATCAGCACCATCTGCTAGGCATTTGTCAACTTCCTACCAGAAAAATT; TAAGAAATTAGCATCCTTCAGGTAGTTAAATGTTCCATTGACATGTGCAAAGTACCTACT; TACGGCAAGTTTTTCTGGGATAATAAACTTCTGTTTATCAAATAAGTTATTTTCTACAAC; CACATCATGGAGTTGATTGACCAGCTGAGTTGCTGCATGACCCTCATCTATTAAATCCTA; TAATAAAAAAAACTTTTGGTATGATGACTTAATATTCCTTTCCCCAAAGTTAGTAAGCTG; ACTTTACCTTGACCACAGCTTCTAATTTGTCAAAAGAGCCACTCTGACAGGCAGCAAATA; CTCCATCAATTTTCTCAGCTGGTATTACCTAAGTAATTGAATGTTCGGTATTAAAGATGT; TTCTAGGTGAGACTAGACATACACTCTGCCAACTGGCCCCCGTGCCCCCAGTCATTTCTG; TTCCACACCTAAGTTCCATACTTTTCTGGGAAGGCTTTGGGAGAACGGAAGGGCCAGAGT; TACTCCAGCCACCACTAAGCAACCAAGTGCTAACAACTGTGCTTTTAAAGCCCTTTTATG; GCCTTGTGAACTTCCAAGTTTGGGCACACATGGATTATAGCCTGNTCTGGTCAGGGGATG; AAGATTAAGTTTTCCCCCTAGCGCTTTACGATTCCTCAAACAGTGGCCAGATCACCACAA; GACTGATTNCTTAAAGGGNAGTGCCTTCTCTCATCTCTCCTGTAAGATGCACTATGAGAT; GGNTTAAAGTCCCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002916
• Insert Size: 2580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002916.3, NP_002907.1
• RefSeq Size: 1427 bp
• RefSeq ORF: 1092 bp
• Locus ID: 5984
• UniProt ID: P35249
• Cytogenetics: 3q27.3
• Domains: AAA
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair
• Summary: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform.