RAF1 (NM_002880) Human Untagged Clone

SKU SC118322

RAF1 (untagged)-Human v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RAF1
• Synonyms: c-Raf; CMD1NN; CRAF; NS5; Raf-1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002880 edited; ATGGAGCACATACAGGGAGCTTGGAAGACGATCAGCAATGGTTTTGGATTCAAAGATGCC; GTGTTTGATGGCTCCAGCTGCATCTCTCCTACAATAGTTCAGCAGTTTGGCTATCAGCGC; CGGGCATCAGATGATGGCAAACTCACAGATCCTTCTAAGACAAGCAACACTATCCGTGTT; TTCTTGCCGAACAAGCAAAGAACAGTGGTCAATGTGCGAAATGGAATGAGCTTGCATGAC; TGCCTTATGAAAGCACTCAAGGTGAGGGGCCTGCAACCAGAGTGCTGTGCAGTGTTCAGA; CTTCTCCACGAACACAAAGGTAAAAAAGCACGCTTAGATTGGAATACTGATGCTGCGTCT; TTGATTGGAGAAGAACTTCAAGTAGATTTCCTGGATCATGTTCCCCTCACAACACACAAC; TTTGCTCGGAAGACGTTCCTGAAGCTTGCCTTCTGTGACATCTGTCAGAAATTCCTGCTC; AATGGATTTCGATGTCAGACTTGTGGCTACAAATTTCATGAGCACTGTAGCACCAAAGTA; CCTACTATGTGTGTGGACTGGAGTAACATCAGACAACTCTTATTGTTTCCAAATTCCACT; ATTGGTGATAGTGGAGTCCCAGCACTACCTTCTTTGACTATGCGTCGTATGCGAGAGTCT; GTTTCCAGGATGCCTGTTAGTTCTCAGCACAGATATTCTACACCTCACGCCTTCACCTTT; AACACCTCCAGTCCCTCATCTGAAGGTTCCCTCTCCCAGAGGCAGAGGTCGACATCCACA; CCTAATGTCCACATGGTCAGCACCACCCTGCCTGTGGACAGCAGGATGATTGAGGATGCA; ATTCGAAGTCACAGCGAATCAGCCTCACCTTCAGCCCTGTCCAGTAGCCCCAACAATCTG; AGCCCAACAGGCTGGTCACAGCCGAAAACCCCCGTGCCAGCACAAAGAGAGCGGGCACCA; GTATCTGGGACCCAGGAGAAAAACAAAATTAGGCCTCGTGGACAGAGAGATTCAAGCTAT; TATTGGGAAATAGAAGCCAGTGAAGTGATGCTGTCCACTCGGATTGGGTCAGGCTCTTTT; GGAACTGTTTATAAGGGTAAATGGCACGGAGATGTTGCAGTAAAGATCCTAAAGGTTGTC; GACCCAACCCCAGAGCAATTCCAGGCCTTCAGGAATGAGGTGGCTGTTCTGCGCAAAACA; CGGCATGTGAACATTCTGCTTTTCATGGGGTACATGACAAAGGACAACCTGGCAATTGTG; ACCCAGTGGTGCGAGGGCAGCAGCCTCTACAAACACCTGCATGTCCAGGAGACCAAGTTT; CAGATGTTCCAGCTAATTGACATTGCCCGGCAGACGGCTCAGGGAATGGACTATTTGCAT; GCAAAGAACATCATCCATAGAGACATGAAATCCAACAATATATTTCTCCATGAAGGCTTA; ACAGTGAAAATTGGAGATTTTGGTTTGGCAACAGTAAAGTCACGCTGGAGTGGTTCTCAG; CAGGTTGAACAACCTACTGGCTCTGTCCTCTGGATGGCCCCAGAGGTGATCCGAATGCAG; GATAACAACCCATTCAGTTTCCAGTCGGATGTCTACTCCTATGGCATCGTATTGTATGAA; CTGATGACGGGGGAGCTTCCTTATTCTCACATCAACAACCGAGATCAGATCATCTTCATG; GTGGGCCGAGGATATGCCTCCCCAGATCTTAGTAAGCTATATAAGAACTGCCCCAAAGCA; ATGAAGAGGCTGGTAGCTGACTGTGTGAAGAAAGTAAAGGAAGAGAGGCCTCTTTTTCCC; CAGATCCTGTCTTCCATTGAGCTGCTCCAACACTCTCTACCGAAGATCAACCGGAGCGCT; TCCGAGCCATCCTTGCATCGGGCAGCCCACACTGAGGATATCAATGCTTGCACGCTGACC; ACGTCCCCGAGGCTGCCTGTCTTCTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002880 unedited; TTGGTTTTGTATCCGACTTCTATAGGCGGCCGCGAATTCGGCACGAGCCGATGTGACCGC; CTCCCGCTCCCTCACCCGCCGCGGGGAGGAGGAGCGGGCGAGAAGCTGCCGCCGAACGAC; AGGACGTTGGGGCGGCCTGGCTCCCTCAGGTTTAAGAATTGTTTAAGCTGCATCAATGGA; GCACATACAGGGAGCTTGGAAGACGATCAGCAATGGTTTTGGATTCAAAGATGCCGTGTT; TGATGGCTCCAGCTGCATCTCTCCTACAATAGTTCAGCAGTTTGGCTATCAGCGCCGGGC; ATCAGATGATGGCAAACTCACAGATCCTTCTAAGACAAGCAACACTATCCGTGTTTTCTT; GCCGAACAAGCAAAGAACAGTGGTCAATGTGCGAAATGGAATGAGCTTGCATGACTGCCT; TATGAAAGCACTCAAGGTGAGGGGCCTGCAACCAGAGTGCTGTGCAGTGTTCAGACTTCT; CCACGAACACAAAGGTAAAAAAGCACGCTTAGATTAGGAATACTAGATGCTGCGTCTTTG; ATTGGNNAGAAGAACTTCAAGTAGATTTCCTGGATCATGTTCCCCTCACAACACACAACT; TTGCTCGGAAGACGTTCCTGAAGCTTGCCTTCTGTGACATCTGTCAGAAATTCCTGCTAA; TGGATTTTCGATGTCAGACTTGTGGCTACAAATTTCATGAGCACTGTAGCACCAAAGTAC; CTACTATGTGTGGTAGACCTTGGAGTAACCATCAGACAACTCTTATTGTTTCCAAATTCC; ACTATTGGTGATAGTGGGAGTCCCAGCCCTACCTTCTTAGACTTTGCGTCGTTTGCGAGA; GTCTGTTTCCAGATGCCTGTTAGTTTTCAGACAGATTTTCTACCCTCACGCCTTCACCTT; AACACCTCCGTCCCTCTTTGAAGGTCCTCTCCAGAGGCAGAGGTCGATTCCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002880 unedited; TGAGGAAGTGGGTCACAGTGGCATGCCCACTCCGGTGTATCTGTATCAGGAAAACAGTCT; ATGCACTCGCGCGCCCCGCAATACTAGTAGTACGAGCTTTTTCTTTTTTTTCTTTTTAAA; TTTAATTTATTTTATTAAAATAACATATTTGAGGGACCATCATATAACTGTTTTTTGTCA; GGTGCAATAAAAACAAAATTAATTTTTTTTTCATCAAGAAAACCTGTTTTCCTGGCTTCC; TTGTATACACATGATGTGACTAAGAAAAACAAGGCTGTTTGTTTGTTTGTTTGATAAAAA; AACAAGGCTGGCCCTGCGGCCCCGCCCCATAGGGGCAGCTCCTGGAAGACAAAATTCAGC; ATGATGGAAGACTGCTCCCTGAGAGGGCTGATATGCGGATTGGCCGAGTGCCTTGCCTGG; AAAACCATCCCAATGCACTGGACACCTTATAATCTGTGAAAGGAGGACGTGTCCCCTAAG; AAAAGTTCCATAGTACCAAAGCAGGCTCCTTCGGGCGGCCAGAGTCTCGGCAGTCCTGGG; CTGTTTGGTGCCTTATGTGCAAAATGTCTGGCGCTGCACCACTCTCTGAAGAAAGTCCCG; CCTGTGACATGCATTCCTCCAGAAGCTGATTTCCAAAATCCCATGTGTCTCCACATCATG; GCTGGACTGCCTGCTACCTTACTTCCTCTAAATACTCATGTACCCAACAGCTGGGGCTGG; GCCCCTGCTTTTTGTCTACCATCAACATCCACTTGCGCATCTACAGAAAGCTGGGCCCTT; GAGCATGGGGTATGTGGGGAGGGAGCAAGACACACCAGCACTGGATATGGCTTCCCTTTT; CCAGGGCCCATGGGNTTGAAAAAAAGGCAATATTATTTTATGCCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002880
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002880.2, NP_002871.1
• RefSeq Size: 3245 bp
• RefSeq ORF: 1947 bp
• Locus ID: 5894
• UniProt ID: P04049
• Cytogenetics: 3p25.2
• Domains: DAG_PE-bind, pkinase, RBD, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway
• Summary: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2), as well as variant 3, encodes isoform b.