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SATB1 (NM_002971) Human Untagged Clone

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SKU
SC118309
SATB1 (untagged)-Human SATB homeobox 1 (SATB1), transcript variant 1
  $700.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SATB1
Synonyms DEFDA; KTZSL
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118309 sequence for NM_002971 edited (data generated by NextGen Sequencing)
ATGGATCATTTGAACGAGGCAACTCAGGGGAAAGAACATTCAGAAATGTCTAACAATGTG
AGTGATCCGAAGGGTCCACCAGCCAAGATTGCCCGCCTGGAGCAGAACGGGAGCCCGCTA
GGAAGAGGAAGGCTTGGGAGTACAGGTGCAAAAATGCAGGGAGTGCCTTTAAAACACTCG
GGCCATCTGATGAAAACCAACCTTAGGAAAGGAACCATGCTGCCAGTTTTCTGTGTGGTG
GAACATTATGAAAACGCCATTGAATATGATTGCAAGGAGGAGCATGCAGAATTTGTGCTG
GTGAGAAAGGATATGCTTTTCAACCAGCTGATCGAAATGGCATTGCTGTCTCTAGGTTAT
TCACATAGCTCTGCTGCCCAGGCCAAAGGGCTAATCCAGGTTGGAAAGTGGAATCCAGTT
CCACTGTCTTACGTGACAGATGCCCCTGATGCTACAGTAGCAGATATGCTTCAAGATGTG
TATCATGTGGTCACATTGAAAATTCAGTTACACAGTTGCCCCAAACTAGAAGACTTGCCT
CCCGAACAATGGTCGCACACCACAGTGAGGAATGCTCTGAAGGACTTACTGAAAGATATG
AATCAGAGTTCATTGGCCAAGGAGTGCCCCCTTTCACAGAGTATGATTTCTTCCATTGTG
AACAGTACTTACTATGCAAATGTCTCAGCAGCAAAATGTCAAGAATTTGGAAGGTGGTAC
AAACATTTCAAGAAGACAAAAGATATGATGGTTGAAATGGATAGTCTTTCTGAGCTATCC
CAGCAAGGCGCCAATCATGTCAATTTTGGCCAGCAACCAGTTCCAGGGAACACAGCCGAG
CAGCCTCCATCCCCTGCGCAGCTCTCCCATGGCAGCCAGCCCTCTGTCCGGACACCTCTT
CCAAACCTGCACCCTGGGCTCGTATCAACACCTATCAGTCCTCAATTGGTCAACCAGCAG
CTGGTGATGGCTCAGCTGCTGAACCAGCAGTATGCAGTGAATAGACTTTTAGCCCAGCAG
TCCTTAAACCAACAATACTTGAACCACCCTCCCCCTGTCAGTAGATCTATGAATAAGCCT
TTGGAGCAACAGGTTTCGACCAACACAGAGGTGTCTTCCGAAATCTACCAGTGGGTACGC
GATGAACTGAAACGAGCAGGAATCTCCCAGGCGGTATTTGCACGTGTGGCTTTTAACAGA
ACTCAGGGCTTGCTTTCAGAAATCCTCCGAAAGGAAGAGGACCCCAAGACTGCATCCCAG
TCTTTGCTGGTAAACCTTCGGGCTATGCAGAATTTCTTGCAGTTACCGGAAGCTGAAAGA
GACCGAATATACCAGGACGAAAGGGAAAGGAGCTTGAATGCTGCCTCGGCCATGGGTCCT
GCCCCACTCATCAGCACACCACCCAGCCGTCCTCCCCAGGTGAAAACAGCTACTATTGCC
ACTGAAAGGAATGGGAAACCAGAGAACAATACCATGAACATTAATGCTTCCATTTATGAT
GAGATTCAGCAGGAAATGAAGCGTGCTAAAGTGTCTCAAGCACTGTTTGCAAAGGTTGCA
GCAACCAAAAGCCAGGGATGGTTGTGCGAGCTGTTACGCTGGAAAGAAGATCCTTCTCCA
GAAAACAGAACCCTGTGGGAGAACCTCTCCATGATCCGAAGGTTCCTCAGTCTTCCTCAG
CCAGAACGTGATGCCATTTATGAACAGGAGAGCAACGCGGTGCATCACCATGGCGACAGG
CCGCCCCACATTATCCATGTTCCAGCAGAGCAGATTCAGCAACAGCAGCAGCAACAGCAA
CAGCAGCAGCAGCAGCAGCAGGCACCGCCGCCTCCACAGCCACAGCAGCAGCCACAGACA
GGCCCTCGGCTCCCCCCACGGCAACCCACGGTGGCCTCTCCAGCAGAGTCAGATGAGGAA
AACCGACAGAAGACCCGGCCACGAACAAAAATTTCAGTGGAAGCCTTGGGAATCCTCCAG
AGTTTCATACAAGACGTGGGCCTGTACCCTGACGAAGAGGCCATCCAGACTCTGTCTGCC
CAGCTCGACCTTCCCAAGTACACCATCATCAAGTTCTTTCAGAACCAGCGGTACTATCTC
AAGCACCACGGCAAACTGAAGGACAATTCCGGTTTAGAGGTCGATGTGGCAGAATATAAA
GAAGAGGAGCTGCTGAAGGATTTGGAAGAGAGTGTCCAAGATAAAAATACTAACACCCTT
TTTTCAGTGAAACTAGAAGAAGAGCTGTCAGTGGAAGGAAACACAGACATTAATACTGAT
TTGAAAGACTGA

Clone variation with respect to NM_002971.4
1386 c=>a
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002971 unedited
NTTTAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGCCTTCAGG
TCTGCTGCTTTTTTATTTTTTTTTTTTAATTAAAAAAAAAAGGACATAGAAAACATCAGT
CTTGAACTTCTCTTCAAGAACCCGGGCTGCAAAGGAAATCTCCTTTGTTTTTGTTATTTA
TGTGCTGTCAAGTTTTGAAGTGGTGAGTTTCAGGTGATCTTTAGACAGTGACTGAGTATG
GATCATTTGAACGAGGCAACTCAGGGGAAAGAACATTCAGAAATGTCTAACAATGTGAGT
GATCCGAAGGGTCCACCAGCCAAGATTGCCCGCCTGGAGCAGAACGGGAGCCCGCTAGGA
AGAGGAAGGCTTGGGAGTACAGGTGCAAAAATGCAGGGAGTGCCTTTAAAACACTCGGGC
CATCTGATGAAAACCAACCTTAGGAAAGGAACCATGCTGCCAGTTTTCTGTGTGGTGGAA
CATTATGAAAACGCCATTGAATATGATTGCAAGGAGGAGCATGCAGAATTTGTGCTGGTG
AGAAAGGATATGCTTTTCAACCAGCTGATCGAAATGGCATTGCTGTCTCTAGGTTATTCA
CATAGCTCTGCTGCCCAGGCCAAAGGGCTAATCCAGGTTGGAAAGTGGAATCCAGTTCCA
CTGTCTTACGTGACAGATGCCCCTGATGCTACAGTAGCAGATATGCTTCAAGATGTGTAT
CATTGTGGTACATTGAAAATTTCAGTACACAGTTGCCCCAACTAGAAAGACTTGCCTNCC
GACCATGGTCCCACACCACATGAGGAATGCTTGAAGGGACTTACTGAAGAATGNTNCAAA
GTTCATGCCAAGGAATGGCCCCCTCACAAATATGATTNCTTCATTGGGGAACGGACTACT
TT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002971 unedited
GGTATGGCAACTTCCAGGGCCAGNANAGGCACTGGGGAGGGGTCACAGGGATGCCACCCG
GGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAGGGGCAAGTTTCCA
AAAATCAGGGGGGAGGGCTCCAAAAATAATTATGGGAGAGGAAATCATAATCCCAAAAGG
TTTAATGCTTGTTTGAGGCTCCGGAATAAAAACTAAAAAAAAAACAAAAAACACTGGTTT
CATGCTTACGGGGTACACACTTTGGGGCATCCCGGGAACCCAAATTTTAATACCAAACAA
TCCTTGATGCTTCACCTGGGGCTGCCAAGCAGTTTGTAAAACAAAGGAAAACATTTAGGG
CAGTCTGTATTATCCTTTTCCAACTTTTCTGTTTGGGCAAGTTTTTGAAAATTCATTGGC
CAAACAATGAACAACAAAGGTTTTCTGAAAAAAAACAAGGGGGACTTTTCATTTTGTTAA
AAAATACCAGGGGCACTGTTAAACAAAACAAATACTTTTATCTCAGTCTTTCAAAACAAT
ATTAAGGGCTGTGTTTCCTTCCACTGACAGCTCTTCTTTTAGTTTCACTGAAAAAGGGTG
TTAGTATTTTTATCTTGGACACTCTCTTCCAAATCCTTAACAGCTCCTCTTCTTTATATT
CTGCCACATTGACCTCTAAACGGGAATTGCCCTTCATTTTGCCCGGGGGCTTGAGAAATA
CCCCTTGGTTCTGAAAAAACTGATGATGGTGACTTGGGGAAGGTCAACTGGCAAAACAAA
TTGGAATGCCCTTTCTCAGGTACAGGCCC
Restriction Sites NotI-NotI |
ACCN NM_002971
Insert Size 2820 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002971.2, NP_002962.1
RefSeq Size 3782 bp
RefSeq ORF 2292 bp
Locus ID 6304
UniProt ID Q01826
Cytogenetics 3p24.3
Domains CUT, homeobox
Protein Families Druggable Genome, Transcription Factors
Summary This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. provided by RefSeq, Apr 2016
Transcript Variant: This variant (1) differs in the 5' UTR and lacks an alternate exon in the 3' coding region, compared to variant 3. Isoform 1 has a shorter C-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_002971" in other vectors (7)
SKU Description Size Price
RC200421 SATB1 (Myc-DDK-tagged)-Human SATB homeobox 1 (SATB1), transcript variant 1 10 ug
$699.00
RC200421L1 Lenti ORF clone of Human SATB homeobox 1 (SATB1), transcript variant 1, Myc-DDK-tagged 10 ug
$999.00
RC200421L2 Lenti ORF clone of Human SATB homeobox 1 (SATB1), transcript variant 1, mGFP tagged 10 ug
$999.00
RC200421L3 Lenti ORF clone of Human SATB homeobox 1 (SATB1), transcript variant 1, Myc-DDK-tagged 10 ug
$999.00
RC200421L4 Lenti ORF clone of Human SATB homeobox 1 (SATB1), transcript variant 1, mGFP tagged 10 ug
$999.00
RG200421 SATB1 (tGFP-tagged) - Human SATB homeobox 1 (SATB1), transcript variant 1 10 ug
$789.00 $899.00
SC320672 SATB1 (untagged)-Human SATB homeobox 1 (SATB1), transcript variant 1 10 ug
$700.00

file-alt Citations

The alterations in SATB1 and nuclear F-actin expression affect apoptotic response of the MCF-7 cells to geldanamycin
Grzanka, Izdebska, Klimaszewska-Wisniewska et al
Folia Histochem. Cytobiol. (2015) 53 (1), 79-87 DOI: 10.5603/FHC.a2015.0008
AI Summary: In this study, OriGene's human SATB1 cDNA cloned into the pCMV6-XL5 expression plasmid (NM_002971) was used to overexpress SATB1 in MCF-7 cells, enabling the researchers to investigate how altered SATB1 levels affect the apoptotic response to geldanamycin.
Snippet: Solution, together with 3 pmol siRNA against human SATB1 (Hs_SATB1_3;Qiagen, Hilden, Germany), 3 pmol siRNA against human cofilin-1 (Hs_CFL1_3), 2 µg human cDNA of SATB1 cloned into pCMV6-XL5 expression plasmid vector (NM_002971;OriGene, Rockville ... Cite SaveSaving
MicroRNA-191, an estrogen-responsive microRNA, functions as an oncogenic regulator in human breast cancer
Nagpal, Ahmad, Molparia et al
Carcinogenesis (2013) 34 (8), 1889-99 DOI: 10.1093/carcin/bgt107
Cited by 122
AI Summary: In this study, the SATB1 cDNA clone from OriGene was used to overexpress SATB1 in breast cancer cells, enabling the researchers to investigate the functional role of SATB1 in the estrogen/miR-191 regulatory pathway.
Snippet: The SATB1 is overexpressed in these stable overexpressing clones by transfecting them with the SATB1 complementary DNA (cDNA) clone (Origene) using lipofectamine ... The specific protein band was visualized by autoradiography using an ECL kit (Amersham ECL Prime).
Deficiency of SATB1 expression in Sezary cells causes apoptosis resistance by regulating FasL/CD95L transcription
Wang, Su, Zhou et al
Blood (2011) 117 (14), 3826-35 DOI: 10.1182/blood-2010-07-294819
Cited by 61
AI Summary: OriGene’s SATB1 cDNA clone (RC206307) was used to restore SATB1 expression in Sézary cells, enabling the study of its role in apoptosis resistance as described in the paper.
The interactions between SATB1 and F-actin are important for mechanisms of active cell death
Grzanka, Kowalczyk
Folia Histochemica et Cytobiologica (2015)
AI Summary: In this study, OriGene products—including human SATB1 cDNA cloned into the pCMV6-XL5 expression plasmid (NM_002971) and another cDNA clone (NM_005507)—were used to investigate the molecular interactions between SATB1 and F-actin during active cell death.
Snippet: siRNA against human cofilin-1 (Hs_CFL1_3), 2 µg human cDNA of SATB1 cloned into pCMV6-XL5 expression plasmid vector (NM_002971;OriGene, Rockville, MD, USA) or 2 ... (NM_005507;OriGene, Maryland, USA), respectively. Then, the mixture was transferred into ... Cite SaveSaving
Showing 1-4 of 4 papers.
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