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RIT2 (NM_002930) Human Untagged Clone

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SKU
SC118279
RIT2 (untagged)-Human Ras-like without CAAX 2 (RIT2)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RIT2
Synonyms RIBA; RIN; ROC2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_002930 edited
ATGGAGGTAGAAAATGAAGCCAGCTGCTCCCCGGGCAGCGCATCAGGCGGGTCCAGAGAG
TACAAGGTGGTAATGCTGGGAGCAGGGGGAGTTGGTAAAAGCGCAATGACAATGCAGTTT
ATTAGTCATCAGTTCCCTGATTATCATGACCCTACTATAGAAGATGCTTATAAGACCCAG
GTCAGGATTGACAATGAGCCAGCTTACTTGGACATCTTGGACACTGCTGGCCAGGCAGAA
TTCACAGCCATGCGGGAGCAGTACATGCGAGGTGGGGAAGGCTTCATCATCTGCTACTCC
GTCACTGACCGTCAATCATTTCAGGAGGCTGCCAAGTTTAAAGAGCTCATTTTTCAGGTC
CGCCACACCTATGAAATTCCCCTGGTGCTGGTGGGTAACAAAATTGATCTGGAACAGTTC
CGCCAGGTTTCTACAGAAGAAGGCTTGAGTCTTGCCCAAGAATATAATTGTGGTTTTTTT
GAGACCTCTGCAGCCCTCAGATTCTGTATTGATGATGCTTTTCATGGCTTAGTGAGGGAA
ATTCGCAAGAAGGAGTCCATGCCATCCTTGATGGAAAAGAAACTGAAGAGAAAAGACAGC
CTGTGGAAGAAGCTCAAAGGTTCTTTGAAGAAGAAGAGAGAAAATATGACATGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_002930 unedited
GTCAGAATTTGTATACGACTCCTATAGGGCGGCCGCGAATCGGCACGAGGGCCCGACGCT
GATGGTTCTTACCTCGTACTAAAACCTTTGCTTTGACACAGTTTTAGAGTTGCTTAATAT
TCGAGCAAGCACCTGACACGGGTGACTTTCTCCTTCTTTTTTTCCTCCGGTCCCTCGGGT
AAGATGGAGGTAGAAAATGAAGCCAGCTGCTCCCCGGGCAGCGCATCAGGCGGGTCCAGA
GAGTACAAGGTGGTAATGCTGGGAGCAGGGGGAGTTGGTAAAAGCGCAATGACAATGCAG
TTTATTAGTCATCAGTTCCCTGATTATCATGACCCTACTATAGAAGATGCTTATAAGACC
CAGGTCAGGATTGACAATGAGCCAGCTTACTTGGACATCTTGGACACTGCTGGCCAGGCA
GAATTCACAGCCATGCGGGAGCAGTACATGCGAGGTGGGGAAGGCTTCATCATCTGCTAC
TCCGTCACTGACCGTCAATCATTTCAGGAGGCTGCCAAGTTTAAAGAGCTCATTTTTCAG
GTCCGCCACACCTATGAAATTCCCCTGGTGCTGGTGGGTAACAAAATTGATCTGGAACAG
TTCCGCCAGGTTTCTACAGAAGAAGGCTTGAGTCTTGCCCAAGAAATAATTGTGGGTTTT
TTTGAGACCTCTGCAGCCCTCAGATTCTGTATTGATGATGCTTTTCATGGCTTAGTGAGG
GAAATTCGCAGGAAGGAGTCCATGCCATCCTTGATGGAAAAGAAACTGAAAGAGAAAGAC
AGCCTGTGGGAGAGCCTCAAAGGTTCTTTGAAGGAGAAGAGAGCAAATATGACATGATAT
NTTGGCTTTTTGGAGGTCCTCACGCTCTCTGAATTTAATATTAGTTGGACAATCCCATAT
TGTAGCATTCTGCTTCATATTATC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002930 unedited
TGGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGGCAATTGGAATG
TCAATTTTATTTAGTACTATGTTGTAAAAACTAAACAGCATATCCAGCTGATTGACAAAA
TCCATCCAACTGTTAAAGGCAAAACAAAACTATCTCAAGAGGTACAGATATGCAGAGCTT
GCTTTTACCTACAGGCAGATATTTAAAGAGAGAGAGACACATAGAGAGATAATATTGAAG
CAGAATGCTACATATGGAATTGTCCAACTAATAAAATTCAGAGAGCGTGAGGAACTCAAA
AGCAAAGATATCATGTCATATTTTCTCTCTTCTTCTTCAAAGAACCTTTGAGCTTCTTCC
ACAGGCTGTCTTTTCTCTTCAGTTTCTTTTCCATCAAGGATGGCATGGACTCCTTCTTGC
GAATTTCCCTCACTAAGCCATGAAAAGCATCATCAATACAGAATCTGAGGGCTGCAGAGG
TCTCAAAAAAACCACAATTATATTCTTGGGCAAGACTCAAGCCTTCTTCTGTAGAAACCT
GGCGGAACTGTTCCAGATCAATTTTGTTACCCACCAGCACCAGGGGAATTTCATAGGTGT
GGCGGACCTGAAAAATGAGCTCTTTAAACTTGGCAGCCTCCTGAAATGATTGACGGTCAG
TGACGGAGTAGCAGATGATGAAGCCTTCCCACCTCGCATGTACTGCTCCCGCATGGCTGT
GAATTCTGCCTGGCCAGCAGTGTCCAAGATGTCCAAGTAAGCTGGCTCATTGTCAATCCT
GACCTGNGTCTTATAAGCATCCTCTATAGTAGGGTCATGATAATCAGGGAACTGATGACT
AATAAACTGCATTGTCATTGCGCTTTTACCAACTNCCCCTGCTCCCANATTTACCACTTG
TACTCTCTGGACCCCCTGAGCGCTGCCCGGGAACACT
Restriction Sites NotI-NotI |
ACCN NM_002930
Insert Size 1090 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002930.1, NP_002921.1
RefSeq Size 1113 bp
RefSeq ORF 654 bp
Locus ID 6014
UniProt ID Q99578
Cytogenetics 18q12.3
Domains RAB, ras, RAS, RHO
Summary RIN belongs to the RAS (HRAS; MIM 190020) superfamily of small GTPases (Shao et al., 1999 PubMed 10545207).supplied by OMIM, Mar 2008
Transcript Variant: This variant (1) encodes the longer isoform (1).
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Documents
Resources
"NM_002930" in other vectors (6)
SKU Description Size Price
RC205367 RIT2 (Myc-DDK-tagged)-Human Ras-like without CAAX 2 (RIT2) 10 ug
$450.00
RC205367L1 Lenti ORF clone of Human Ras-like without CAAX 2 (RIT2), Myc-DDK-tagged 10 ug
$750.00
RC205367L2 Lenti ORF clone of Human Ras-like without CAAX 2 (RIT2), mGFP tagged 10 ug
$750.00
RC205367L3 Lenti ORF clone of Human Ras-like without CAAX 2 (RIT2), Myc-DDK-tagged 10 ug
$750.00
RC205367L4 Lenti ORF clone of Human Ras-like without CAAX 2 (RIT2), mGFP tagged 10 ug
$750.00
RG205367 RIT2 (tGFP-tagged) - Human Ras-like without CAAX 2 (RIT2) 10 ug
$650.00

file-alt Citations

The small GTPase Rit2 modulates LRRK2 kinase activity, is required for lysosomal function and protects against alpha-synuclein neuropathology
Obergasteiger, Castonguay, Pizzi et al
NPJ Parkinsons Dis (2023) 9 (1), 44 DOI: 10.1038/s41531-023-00484-2
Cited by 26
AI Summary: OriGene products contributed to this study by providing the eGFP plasmid (SC118279) for cell transfection and the rabbit anti-Rit2 antibody (TA501701) for co-immunoprecipitation experiments, enabling the investigation of Rit2’s role in LRRK2 kinase activity and lysosomal function.
Snippet: …Briefly, 200,000 cells were resuspended in the nucleofection solution containing plasmid DNA (SC118279, Origene, eGFP in pcDNA3.1, 800 ng) and analyzed after 72 h. For CO-IP experiments, 2 × 106 cells were resuspended in 100 μl of the nucleofection solution containing 2 μg of plasmid DNA;- After pre-clearing, 0.5 mg of protein extracts were incubated overnight at 4 C with 5 μg of rabbit anti-Rit2 antibody (Origene, TA501701), or with same species IgG (Life
FigureFigureFigure
IP
Évaluation du rôle neuroprotecteur de la petite GTPase Rin pour le traitement de la maladie de Parkinson
Castonguay
Thesis (2020)
Snippet: …Sc.) Quebec;En bref, 200 000 cellules ont été mises en suspension dans la solution de nucléofection contenant 800 ng d'ADN plasmidique encodant RIT2 humain (SC118279, Origene), et incubées pendant 10 minutes après le début la nucléofection. …
Showing 1-2 of 2 papers.
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