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SFRP4 (NM_003014) Human Untagged Clone

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SKU
SC118247
SFRP4 (untagged)-Human secreted frizzled-related protein 4 (SFRP4)
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SFRP4
Synonyms FRP-4; FRPHE; FRZB-2; PYL; sFRP-4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC118247 sequence for NM_003014 edited (data generated by NextGen Sequencing)
ATGTTCCTCTCCATCCTAGTGGCGCTGTGCCTGTGGCTGCACCTGGCGCTGGGCGTGCGC
GGCGCGCCCTGCGAGGCGGTGCGCATCCCTATGTGCCGGCACATGCCCTGGAACATCACG
CGGATGCCCAACCACCTGCACCACAGCACGCAGGAGAACGCCATCCTGGCCATCGAGCAG
TACGAGGAGCTGGTGGACGTGAACTGCAGCGCCGTGCTGCGCTTCTTCCTCTGTGCCATG
TACGCGCCCATTTGCACCCTGGAGTTCCTGCACGACCCTATCAAGCCGTGCAAGTCGGTG
TGCCAACGCGCGCGCGACGACTGCGAGCCCCTCATGAAGATGTACAACCACAGCTGGCCC
GAAAGCCTGGCCTGCGACGAGCTGCCTGTCTATGACCGTGGCGTGTGCATCTCGCCTGAA
GCCATCGTCACGGACCTCCCGGAGGATGTTAAGTGGATAGACATCACACCAGACATGATG
GTACAGGAAAGGCCTCTTGATGTTGACTGTAAACGCCTAAGCCCCGATCGGTGCAAGTGT
AAAAAGGTGAAGCCAACTTTGGCAACGTATCTCAGCAAAAACTACAGCTATGTTATTCAT
GCCAAAATAAAAGCTGTGCAGAGGAGTGGCTGCAATGAGGTCACAACGGTGGTGGATGTA
AAAGAGATCTTCAAGTCCTCATCACCCATCCCTCGAACTCAAGTCCCGCTCATTACAAAT
TCTTCTTGCCAGTGTCCACACATCCTGCCCCATCAAGATGTTCTCATCATGTGTTACGAG
TGGCGTTCAAGGATGATGCTTCTTGAAAATTGCTTAGTTGAAAAATGGAGAGATCAGCTT
AGTAAAAGATCCATACAGTGGGAAGAGAGGCTGCAGGAACAGCGGAGAACAGTTCAGGAC
AAGAAGAAAACAGCCGGGCGCACCAGTCGTAGTAATCCCCCCAAACCAAAGGGAAAGCCT
CCTGCTCCCAAACCAGCCAGTCCCAAGAAGAACATTAAAACTAGGAGTGCCCAGAAGAGA
ACAAACCCGAAAAGAGTGTGA

Clone variation with respect to NM_003014.3
786 c=>t
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003014 unedited
AGGTTACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGAGGATTTC
TTCCTCGGCGAAGGACAGCGAAAGAGAGGGTGGCAGGAAGAGAAGGGCGCTTTCTGTCTG
CCGGGGTCGCAGCGCGAGAGGGCAGTGCCATGTTCCTCTCCATCCTAGTGGCGCTGTGCC
TGTGGCTGCACCTGGCGCTGGGCGTGCGCGGCGCGCCCTGCGAGGCGGTGCGCATCCCTA
TGTGCCGGCACATGCCCTGGAACATCACGCGGATGCCCAACCACCTGCACCACAGCACGC
AGGAGAACGCCATCCTGGCCATCGAGCAGTACGAGGAGCTGGTGGACGTGAACTGCAGCG
CCGTGCTGCGCTTCTTCCTCTGTGCCATGTACGCGCCCATTTGCACCCTGGAGTTCCTGC
ACGACCCTATCAAGCCGTGCAAGTCGGTGTGCCAACGCGCGCGCGACGACTGCGAGCCCC
TCATGAAGATGTACAACCACAGCTGGCCCGAAAGCCTGGCCTGCGACGAGCTGCCTGTCT
ATGACCGTGGCGTGTGCATCTCGCCTGAAGCCATCGTCACGGACCTCCCGGAGGATGTTA
AGTGGATAGACATCACACCAGACATGATGGTACAGGAAAGGCCTCTTGATGTTGACTGTA
AACGCCTAAGCCCCGATCGGTGCAAGTGTAAAAAGGTGAAGCCAACTTTGGCAACGTATC
TCAGCAAAACTACAGCTATGTTATTCATGCAAAAATAAAGCTGTGCAGAGGAGTGGGCTG
CATGAAGTCACAACGGTGGTGGATGTAAAAGAGATCTTNCAGTNCTCATCACCCATCCCT
CGAACTTCAGTCCCGCTCATTACAATTCTTTCTGGCAGTGTCCACACATCCTGCCCCATC
AAGATGTTCTCATA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003014 unedited
GGCCGCATTTTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCCTCTCCTTTTTATTTATTTT
TCTTTATTATTATTTTTGTTTTTAGAGGTTCTAGCTAAAAACAGAGCTGAAGTCATTGTA
AAAAAGACACATTATAATTTTCCATATGCTACTTCTCTAGATTTTTCAAAAAATCCCCTT
TCATCACAAAAATCCTTCTGCAGACCATAAACACATAAAGATCTTTTAGACATTCATTCC
ACTACTGTTCCTTCTTAGAAGCATTATATTTTCGATCACATTTTAAAACATCACTAACAT
TGCAACAACAGATATCAAAAGGTAATAAAACATACTGTAAAAAAATTACGTGTTTTGTTT
GAAATATGGCATTTATTTTACAATGCACATATTAGGTCGAATTGTAACAAGCATTATTTT
CCCTACTCTTCTAGAGTATGCACACAGGTTACTCTGAATGCAATGCAATAAGCCTTTTCC
ACCAGCTTTAACTCACCTTCTGTACCAAAGGGCAAACCTACCACTATGGCTTGTGATGGC
TTACAAAGAAAAACTGAAGCATAGCCTTAAGAAAAATGCTGCAAGATGTGTCTATGAAGA
GCACTGCAGTGAGTTGTTAGGGCAAGGGGCACATGGCCTTACATAGGCTGTCCCAGGCAA
TGCCCAGCCTCATCCTGTAAGGAAGTCGGAAGTCTCCGCTTTGGAAACTAGTTAGCTCAC
ACTCTCTTCGGNTTTGTTCTCTTCTGGCACTCCTANGTCTATGGTCTTCTTGGGACTGGC
TGGNTTAGGAGCAGGAGGCTTTCCCTTTGTTTGGGGGGATACTACGACTGGNTGCGCCGG
CTGTTTTCTTTCTGTCCTGAACTGTNTACGCTGTTCCTGCAGCTCTCTTCCACTGTATGG
ATCGTTTACTAGCTGATCNTCCATTTTTAACTAGCAATTTCAAGAGCATCTCCCTGTACG
CACTCGTACCCATGAGAAACATCTGGG
Restriction Sites NotI-NotI |
ACCN NM_003014
Insert Size 1860 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003014.2, NP_003005.1
RefSeq Size 2820 bp
RefSeq ORF 1041 bp
Locus ID 6424
UniProt ID Q6FHJ7
Cytogenetics 7p14.1
Domains FRI, NTR
Protein Families Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
Protein Pathways Wnt signaling pathway
Summary Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_003014" in other vectors (6)
SKU Description Size Price
RC205365 SFRP4 (Myc-DDK-tagged)-Human secreted frizzled-related protein 4 (SFRP4) 10 ug
$457.00
RC205365L1 Lenti ORF clone of Human secreted frizzled-related protein 4 (SFRP4), Myc-DDK-tagged 10 ug
$757.00
RC205365L2 Lenti ORF clone of Human secreted frizzled-related protein 4 (SFRP4), mGFP tagged 10 ug
$757.00
RC205365L3 Lenti ORF clone of Human secreted frizzled-related protein 4 (SFRP4), Myc-DDK-tagged 10 ug
$757.00
RC205365L4 Lenti ORF clone of Human secreted frizzled-related protein 4 (SFRP4), mGFP tagged 10 ug
$757.00
RG205365 SFRP4 (tGFP-tagged) - Human secreted frizzled-related protein 4 (SFRP4) 10 ug
$489.00 $657.00

file-alt Citations

Pyle disease - Functional validation of disease-causing missense variants in SFRP4/sFRP4
Magnus, Hordyjewska-Kowalczyk, Sowińska-Seidler et al
Bone (2026)
AI Summary: OriGene’s human wild-type full-length SFRP4 expression construct (pCMV6-XL5-SFRP4, #SC118247) was used to generate and study the functional effects of SFRP4 missense variants implicated in Pyle disease in this research.
Snippet: A human wild-type full-length SFRP4 expression construct (NM_003014, pCMV6-XL5-SFRP4, #SC118247, OriGene Technologies) was used to introduce selected variants of interest. All variants (c.161CA, p.(Ala54Asp)
Showing 1-1 of 1 papers.
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