SMARCE1 (NM_003079) Human Untagged Clone
SKU
SC118214
SMARCE1 (untagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1)
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | SMARCE1 |
| Synonyms | BAF57; CSS5 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_003079 |
| Insert Size | 2550 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003079.4, NP_003070.3 |
| RefSeq Size | 2425 bp |
| RefSeq ORF | 1236 bp |
| Locus ID | 6605 |
| UniProt ID | Q969G3 |
| Cytogenetics | 17q21.2 |
| Domains | HMG |
| Protein Families | Transcription Factors |
| Summary | The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003079" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209444 | SMARCE1 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1) | 10 ug |
$686.00
|
|
| RC209444L1 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC209444L2 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), mGFP tagged | 10 ug |
$986.00
|
|
| RC209444L3 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), Myc-DDK-tagged | 10 ug |
$986.00
|
|
| RC209444L4 | Lenti ORF clone of Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1), mGFP tagged | 10 ug |
$986.00
|
|
| RG209444 | SMARCE1 (tGFP-tagged) - Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (SMARCE1) | 10 ug |
$489.00
$886.00
|
|
Citations
SWI/SNF regulates the cellular response to hypoxia
Kenneth, Mudie, van Uden et al
J Biol Chem (2009) 284 (7), 4123-31 DOI: 10.1074/jbc.M808491200
Kenneth, Mudie, van Uden et al
J Biol Chem (2009) 284 (7), 4123-31 DOI: 10.1074/jbc.M808491200
AI Summary: In this study, OriGene products—including the BRG1 (SMARCA4) and BRM (SMARCA2) expression plasmids—were used to modulate SWI/SNF complex components, enabling the authors to investigate their role in regulating hypoxia-inducible factor-1alpha expression and the cellular response to hypoxia.
Showing 1-1 of 1 papers.
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