TFF1 (NM_003225) Human Untagged Clone

SKU SC118099

TFF1 (untagged)-Human trefoil factor 1 (TFF1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TFF1
• Synonyms: BCEI; D21S21; HP1.A; HPS2; pNR-2; pS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003225 edited; ATGGCCACCATGGAGAACAAGGTGATCTGCGCCCTGGTCCTGGTGTCCATGCTGGCCCTC; GGCACCCTGGCCGAGGCCCAGACAGAGACGTGTACAGTGGCCCCCCGTGAAAGACAGAAT; TGTGGTTTTCCTGGTGTCACGCCCTCCCAGTGTGCAAATAAGGGCTGCTGTTTCGACGAC; ACCGTTCGTGGGGTCCCCTGGTGCTTCTATCCTAATACCATCGACGTCCCTCCAGAAGAG; GAGTGTGAATTTTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003225 unedited; NGGTCAGGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTTTGGAG; CAGAGAGGAGGCAATGGCCACCATGGAGAACAAGGTGATCTGCGCCCTGGTCCTGGTGTC; CATGCTGGCCCTCGGCACCCTGGCCGAGGCCCAGACAGAGACGTGTACAGTGGCCCCCCG; TGAAAGACAGAATTGTGGTTTTCCTGGTGTCACGCCCTCCCAGTGTGCAAATAAGGGCTG; CTGTTTCGACGACACCGTTCGTGGGGTCCCCTGGTGCTTCTATCCTAATACCATCGACGT; CCCTCCAGAAGAGGAGTGTGAATTTTAGACACTTCTGCAGGGATCTGCCTGCATCCTGAC; GCGGTGCCGTCCCCAGCACGGTGATTAGTCCCAGAGCTCGGCTGCCACCTCCACCGGACA; CCTCAGACACGCTTCTGCAGCTGTGCCTCGGCTCACAACACAGATTGACTGCTCTGACTT; TGACTACTCAAAATTGGCCTAAAAATTAAAAGAGATCGATATTAAAAAAAAAAAAAAAAA; ACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCAT; CCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGNAAGTTGCCACTCCAGTGCCCACC; AGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATA; TTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGGCAGTTGGGAAGACAACCTGTTAGG; CCTGCGGGGTCTATTGGGAACCAACCTGGAGTGCAGTGGCACAATCTTGGCTCACTTGCA; TCTCCGCCTCCTGGGTTCAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003225 unedited; ATGTACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTAATATCGATCTC; TTTTAATTTTTAGGCCAATTTTGAGTAGTCAAAGTCAGAGCAGTCAATCTGTGTTGTGAG; CCGAGGCACAGCTGCAGAAGCGTGTCTGAGGTGTCCGGTGGAGGTGGCAGCCGAGCTCTG; GGACTAATCACCGTGCTGGGGACGGCACCGCGTCAGGATGCAGGCAGATCCCTGCAGAAG; TGTCTAAAATTCACACTCCTCTTCTGGAGGGACGTCGATGGTATTAGGATAGAAGCACCA; GGGGACCCCACGAACGGTGTCGTCGAAACAGCAGCCCTTATTTGCACACTGGGAGGGCGT; GACACCAGGAAAACCACAATTCTGTCTTTCACGGGGGGCCACTGTACACGTCTCTGTCTG; GGCCTCGGCCAGGGTGCCGAGGGCCATCATGGACACCAGGACCAGGGCGCAGATCACCTT; GTTCTCCATGGTGGCCATTGCCTCCTCTCTGCTCCAAAGCCTCGTGCCGAATTCGCGGCC; GCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATAT; AGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGAGCGGAGTTATTACGAC; ATTTTGGAAAGTNCCGTTGATTTTGGTCGCAAAACAAACTCCCATTGACGTCAATGGGGG; TGGAGACTTGGAAATTCCCGGGAGTCAAACCGCTTTCCACGCCCCTTGGTGTACTGGCCA; AAACCGCTCACCATGGTAATAGCGATGACTCATACGTAAATGTACTGCCANGTAAGAATA; GTCCCGTAGGGCATTGTCTTGGCATAATTGCCAGCCGGCGCCTTTATCGCCTTTGGCGTA; ATTAGGGGCCGGGACTTGCCTTTGATACACTTGATGAACTGGCAAGTGGGCATTTTACCG; TAATTCTCCACCCTTTGCCCCATAGGAAAGTCCCTTTGGGGGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_003225
• Insert Size: 500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003225.2, NP_003216.1
• RefSeq Size: 508 bp
• RefSeq ORF: 255 bp
• Locus ID: 7031
• UniProt ID: P04155
• Cytogenetics: 21q22.3
• Domains: PD
• Protein Families: Druggable Genome, Secreted Protein
• Summary: Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]