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THBS4 (NM_003248) Human Untagged Clone

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SKU
SC118077
THBS4 (untagged)-Human thrombospondin 4 (THBS4)
  $1,321.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol THBS4
Synonyms TSP-4; TSP4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_003248, the custom clone sequence may differ by one or more nucleotides


ATGCTGGCCCCGCGCGGAGCCGCCGTCCTCCTGCTGCACCTGGTCCTGCAGCGGTGGCTAGCGGCAGGCG
CCCAGGCCACCCCCCAGGTCTTTGACCTTCTCCCATCTTCCAGTCAGAGGCTAAACCCAGGCGCTCTGCT
GCCAGTCCTGACAGACCCCGCCCTGAATGATCTCTATGTGATTTCCACCTTCAAGCTGCAGACTAAAAGT
TCAGCCACCATCTTCGGTCTTTACTCTTCAACTGACAACAGTAAATATTTTGAATTTACTGTGATGGGAC
GCTTAAACAAAGCCATCCTCCGTTACCTGAAGAACGATGGGAAGGTGCATTTGGTGGTTTTCAACAACCT
GCAGCTGGCAGACGGAAGGCGGCACAGGATCCTCCTGAGGCTGAGCAATTTGCAGCGAGGGGCCGGCTCC
CTAGAGCTCTACCTGGACTGCATCCAGGTGGATTCCGTTCACAATCTCCCCAGGGCCTTTGCTGGCCCCT
CCCAGAAACCTGAGACCATTGAATTGAGGACTTTCCAGAGGAAGCCACAGGACTTCTTGGAAGAGCTGAA
GCTGGTGGTGAGAGGCTCACTGTTCCAGGTGGCCAGCCTGCAAGACTGCTTCCTGCAGCAGAGTGAGCCA
CTGGCTGCCACAGGCACAGGGGACTTTAACCGGCAGTTCTTGGGTCAAATGACACAATTAAACCAACTCC
TGGGAGAGGTGAAGGACCTTCTGAGACAGCAGGTTAAGGAAACATCATTTTTGCGAAACACCATAGCTGA
ATGCCAGGCTTGCGGTCCTCTCAAGTTTCAGTCTCCGACCCCAAGCACGGTGGTGCCCCCGGCTCCCCCT
GCACCGCCAACACGCCCACCTCGTCGGTGTGACTCCAACCCATGTTTCCGAGGTGTCCAATGTACCGACA
GTAGAGATGGCTTCCAGTGTGGGCCCTGCCCCGAGGGCTACACAGGAAACGGGATCACCTGTATTGATGT
TGATGAGTGCAAATACCATCCCTGCTACCCGGGCGTGCACTGCATAAATTTGTCTCCTGGCTTCAGATGT
GACGCCTGCCCAGTGGGCTTCACAGGGCCCATGGTGCAGGGTGTTGGGATCAGTTTTGCCAAGTCAAACA
AGCAGGTCTGCACTGACATTGATGAGTGTCGAAATGGAGCGTGCGTTCCCAACTCGATCTGCGTTAATAC
TTTGGGATCTTACCGCTGTGGGCCTTGTAAGCCGGGGTATACTGGTGATCAGATAAGGGGATGCAAAGCG
GAAAGAAACTGCAGAAACCCAGAGCTGAACCCTTGCAGTGTGAATGCCCAGTGCATTGAAGAGAGGCAGG
GGGATGTGACATGTGTGTGTGGAGTCGGTTGGGCTGGAGATGGCTATATCTGTGGAAAGGATGTGGACAT
CGACAGTTACCCCGACGAAGAACTGCCATGCTCTGCCAGGAACTGTAAAAAGGACAACTGCAAATATGTG
CCAAATTCTGGCCAAGAAGATGCAGACAGAGATGGCATTGGCGACGCTTGTGACGAGGATGCTGACGGAG
ATGGGATCCTGAATGAGCAGGATAACTGTGTCCTGATTCATAATGTGGACCAAAGGAACAGCGATAAAGA
TATCTTTGGGGATGCCTGTGATAACTGCCTGAGTGTCTTAAATAACGACCAGAAAGACACCGATGGGGAT
GGAAGAGGAGATGCCTGTGATGATGACATGGATGGAGATGGAATAAAAAACATTCTGGACAACTGCCCAA
AATTTCCCAATCGTGACCAACGGGACAAGGATGGTGATGGTGTGGGGGATGCCTGTGACAGTTGTCCTGA
TGTCAGCAACCCTAACCAGTCTGATGTGGATAATGATCTGGTTGGGGACTCCTGTGACACCAATCAGGAC
AGTGATGGAGATGGGCACCAGGACAGCACAGACAACTGCCCCACCGTCATTAACAGTGCCCAGCTGGACA
CCGATAAGGATGGAATTGGTGACGAGTGTGATGATGATGATGACAATGATGGTATCCCAGACCTGGTGCC
CCCTGGACCAGACAACTGCCGGCTGGTCCCCAACCCAGCCCAGGAGGATAGCAACAGCGACGGAGTGGGA
GACATCTGTGAGTCTGACTTTGACCAGGACCAGGTCATCGATCGGATCGACGTCTGCCCAGAGAACGCAG
AGGTCACCCTGACCGACTTCAGGGCTTACCAGACCGTGGTCCTGGATCCTGAAGGGGATGCCCAGATCGA
TCCCAACTGGGTGGTCCTGAACCAGGGCATGGAGATTGTACAGACCATGAACAGTGATCCTGGCCTGGCA
GTGGGGTACACAGCTTTTAATGGAGTTGACTTCGAAGGGACCTTCCATGTGAATACCCAGACAGATGATG
ACTATGCAGGCTTTATCTTTGGCTACCAAGATAGCTCCAGCTTCTACGTGGTCATGTGGAAGCAGACGGA
GCAGACATATTGGCAAGCCACCCCATTCCGAGCAGTTGCAGAACCTGGCATTCAGCTCAAGGCTGTGAAG
TCTAAGACAGGTCCAGGGGAGCATCTCCGGAACTCCCTGTGGCACACGGGGGACACCAGTGACCAGGTCA
GGCTGCTGTGGAAGGACTCCAGGAATGTGGGCTGGAAGGACAAGGTGTCCTACCGCTGGTTCCTACAGCA
CAGGCCCCAGGTGGGCTACATCAGGGTACGATTTTATGAAGGCTCTGAGTTGGTGGCTGACTCTGGCGTC
ACCATAGACACCACAATGCGTGGAGGCCGACTTGGCGTTTTCTGCTTCTCTCAAGAAAACATCATCTGGT
CCAACCTCAAGTATCGCTGCAATGACACCATCCCTGAGGACTTCCAAGAGTTTCAAACCCAGAATTTCGA
CCGCTTCGATAATTAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_003248 unedited
GTTCGAATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCCCAGCACCG
CGCGGCGGGGACGCGAGCGCGCCCCCGACGGCAGCCCGGACGCCGAGCACGGGTCACCTG
CGGCGCCGGCCCGGGCGCCGACCGAGGTTCAACGCACGGCCCGGGGACCCCCAGGCGGGG
CCAACGCCGCCGTCGCCCCCGGCCTCGCGGGGAGCAGGAAGAGCCAACATGCTGGCCCCG
CGCGGAGCCGCCGTCCTCCTGCTGCACCTGGTCCTGCAGCGGTGGCTAGCGGCAGGCGCC
CAGGCCACCCCCCAGGTCTTTGACCTTCTCCCATCTTCCAGTCAGAGGCTAAACCCAGGC
GCTCTGCTGCCAGTCCTGACAGACCCCGCCCTGAATGATCTCTATGTGATTTCCACCTTC
AAGCTGCAGACTAAAAGTTCAGCCACCATCTTCGGTCTTTACTCTTCAACTGACAACAGT
AAATATTTTGAATTTACTGTGATGGGACGCTTAAACAAAGCCATCCTCCGTTACCTGAAG
AACGATGGGAAGGTGCATTTGGTGGTTTTCAACAACCTGCAGCTGGCAGACGGAAGGCGG
CACAGGATCCTCCTGAGGCTGAGCAATTTGCAGCGAGGGGCCGGCTCCCTAGAGCTCTAC
CTGGACTGCATCCAGGTGGATTCCGTTCACAATCTCCCCAGGGCCTTTGCTGGCCCCTCC
CAGAAACCTGAGACCATTTGAATTGAGGACTTTCCAGAGGAAGCCTCAGGACTTCTTGGN
AAGAGCTGAGCTGGTGGGTGAGAGGCTCACTGGTCNCCAGTGGCCAGCCTGGCAGACTGC
TTCTGCAGCAGAGTGAGCCACTGGCTTGCCAGCACAGGGGACTATACCGGCAGTTTTTGG
GGTCAATGACACATAACCATCTGG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003248 unedited
GCCGCTATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAATGATCTC
TTCTCCTTTATTGCCACATTCACATAAAACGTTTTGATATATTTGGGTTGGTAAAAGCTA
AAGAAAATTGAAGTTAAAATATATATGGTTTTAGTGTTCCGAAAAGCAGTTACAGATTGC
TTCCTTGGTTTAATTATCGAAGCGGTCGAAATTCTGGGTTTGAAACTCTTGGAAGTCCTC
AGGGATGGTGTCATTGCAGCGATACTTGAGGTTGGACCAAATGATGTTTTCTTGAGAGAA
GCAGAAAACGCCAAGTCGGCCTCCACGCATTGTGGTGTCTATGGTGACGCCAGAGTCAGC
CACCAACTCAGAGCCTTCATAAAATCGTACCCTGATGTAGCCCACCTGGGGCCTGTGCTG
TAGGAACCAGCGGTAGGACACCTTGTCCTTCCAGCCCACATTCCTGGAGTCCTTCCACAG
CAGCCTGACCTGGTCACTGGTGTCCCCCGTGTGCCACAGGGAGTTCCGGAGATGCTCCCC
TGGACCTGTCTTAAACTTCACAGCCTTGAGCTGAATGCCAGGTTCTGCAACTGCCTCGGA
ATGGGGTGGCCTTGCCAATATGTCTGCTCCGTCTGCTTCCAATGACCCACCTAAAACCGG
CAGCTATCTTGGGACCCAAAAATAAACCCGCATAAGTCTTATCTGTCTGGGTTTCACATG
GAAGGCCCTTTGAAGCCCCTCCTTAAAACCTGGACCCCCCTGCCAGCCACATCCTGTTCA
GGGCTGGCAATTCCATGCCCTGTTCAGGCCACCCATTGGGACCATCTGGCTTCCCTTTGG
ATCCACACCCAGTCTGTACTCCTTTATCGCTAAGGGACCTTCCGTTCTTGGTGAAACTGC
ATCTCCGAAACCTGGCCTCGTCAAATCCACCCATATGCTCCCTCTTTCGATTACCTCTCT
ACGCGTGGAT
Restriction Sites NotI-NotI |
ACCN NM_003248
Insert Size 3330 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003248.3, NP_003239.2
RefSeq Size 3233 bp
RefSeq ORF 2886 bp
Locus ID 7060
UniProt ID P35443
Cytogenetics 5q14.1
Domains EGF, EGF_CA, TSPN, tsp_3
Protein Families Druggable Genome
Protein Pathways ECM-receptor interaction, Focal adhesion, TGF-beta signaling pathway
Summary The protein encoded by this gene belongs to the thrombospondin protein family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. It is involved in local signaling in the developing and adult nervous system, and it contributes to spinal sensitization and neuropathic pain states. This gene is activated during the stromal response to invasive breast cancer. It may also play a role in inflammatory responses in Alzheimer's disease. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2015
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).
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"NM_003248" in other vectors (4)
SKU Description Size Price
RC208408 THBS4 (Myc-DDK-tagged)-Human thrombospondin 4 (THBS4) 10 ug
$589.00 $880.00
RC208408L3 Lenti-ORF clone of THBS4 (Myc-DDK-tagged)-Human thrombospondin 4 (THBS4) 10 ug
$1,180.00
RC208408L4 Lenti-ORF clone of THBS4 (mGFP-tagged)-Human thrombospondin 4 (THBS4) 10 ug
$1,180.00
RG208408 THBS4 (tGFP-tagged) - Human thrombospondin 4 (THBS4) 10 ug
$1,080.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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