GFUS (NM_003313) Human Untagged Clone

SKU SC118067

TSTA3 (untagged)-Human tissue specific transplantation antigen P35B (TSTA3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GFUS
• Synonyms: FX; P35B; SDR4E1; TSTA3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118067 sequence for NM_003313 edited (data generated by NextGen Sequencing); ATGGGTGAACCCCAGGGATCCATGCGGATTCTAGTGACAGGGGGCTCTGGGCTGGTAGGC; AAAGCCATCCAGAAGGTGGTAGCAGATGGAGCTGGACTTCCTGGAGAGGACTGGGTGTTT; GTCTCCTCTAAAGACGCCGATCTCACGGATACAGCACAGACCCGCGCCCTGTTTGAGAAG; GTCCAACCCACACACGTCATCCATCTTGCTGCAATGGTGGGGGGCCTGTTCCGGAATATC; AAATACAATTTGGACTTCTGGAGGAAAAACGTGCACATGAACGACAACGTCCTGCACTCG; GCCTTTGAGGTGGGCGCCCGCAAGGTGGTGTCCTGCCTGTCCACCTGTATCTTCCCTGAC; AAGACGACCTACCCGATAGATGAGACCATGATCCACAATGGGCCTCCCCACAACAGCAAT; TTTGGGTACTCGTATGCCAAGAGGATGATCGACGTGCAGAACAGGGCCTACTTCCAGCAG; TACGGCTGCACCTTCACCGCTGTCATCCCCACCAACGTCTTCGGGCCCCACGACAACTTC; AACATCGAGGATGGCCACGTGCTGCCTGGCCTCATCCACAAGGTGCACCTGGCCAAGAGC; AGCGGCTCGGCCCTGACGGTGTGGGGTACAGGGAATCCGCGGAGGCAGTTCATATACTCG; CTGGACCTGGCCCAGCTCTTTATCTGGGTCCTGCGGGAGTACAATGAAGTGGAGCCCATC; ATCCTCTCCGTGGGCGAGGAAGATGAGGTCTCCATCAAGGAGGCAGCCGAGGCGGTGGTG; GAGGCCATGGACTTCCATGGGGAAGTCACCTTTGATACAACCAAGTCGGATGGGCAGTTT; AAGAAGACAGCCAGTAACAGCAAGCTGAGGACCTACCTGCCCGACTTCCGGTTCACACCC; TTCAAGCAGGCGGTGAAGGAGACCTGTGCTTGGTTCACTGACAACTACGAGCAGGCCCGG; AAGTGA; ; Clone variation with respect to NM_003313.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003313 unedited; ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGAGCGCACCCGGCCGGA; AGCCGCTGTCGGGGAGCCGGCGGTGGGGCTGGACGCAGGTGCAACTGACATGGGTGAACC; CCAGGGATCCATGCGGATTCTAGTGACAGGGGGCTCTGGGCTGGTAGGCAAAGCCATCCA; GAAGGTGGTAGCAGATGGAGCTGGACTTCCTGGAGAGGACTGGGTGTTTGTCTCCTCTAA; AGACGCCGATCTCACGGATACAGCACAGACCCGCGCCCTGTTTGAGAAGGTCCAACCCAC; ACACGTCATCCATCTTGCTGCAATGGTGGGGGGCCTGTTCCGGAATATCAAATACAATTT; GGACTTCTGGAGGAAAAACGTGCACATGAACGACAACGTCCTGCACTCGGCCTTTGAGGT; GGGCGCCCGCAAGGTGGTGTCCTGCCTGTCCACCTGTATCTTCCCTGACAAGACGACCTA; CCCGATAGATGAGACCATGATCCACAATGGGCCTCCCCACAACAGCAATTTTGGGTACTC; GTATGCCAAGAGGATGATCGACGTGCAGAACAGGGCCTACTTCCAGCAGTACGGCTGCAC; CTTCACCGCTGTCATCCCCACCAACGTCTTCGGGCCCCACGACAACTTCAACATCGNAGA; TGGCCACGTGCTGCCTGGCCTCATCCACAAGGTGCACCTGGCCAAGAGCAGCGGCTCGGC; CCTGACGGTNGTGGGTACAGGGGATCCGCGGAGGCAGTTCATATACTCGCTGGACCTGGC; CCAGCTCTTTATCTGGNGTCCTGCCGGAGACAATGAAGTGGAGCCCATCATCCTCTNCGT; GGGCGAAGAGATGAAGGTCTCCATCAAGGAGCANNCGANNGCGTGGTGGAGCCATGGACT; TCATGGGAAGTCACCTTGATCCACAGTCGNAGGGCAGTTAGAAGAAGCCGTACAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003313 unedited; GTACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCCTGTGAAAATGC; ACTTTATTGGCTCCCAGGAGTGGGATGCAGGATCAGAGTGGACACGCGCAGGGGGCTGGT; GTGGGGAGCAAAGCGCCGGGCCTGCCCGGGACCCTGGTTTCCCTGAGGACCAACGTGAAA; TGGGGGCCCCCACTTGGAAAAAAAGCTTTGGGGCTTCAAAAACCGGTTGGAAATGGCAGG; CCCCCACTTTCCTGGGGGGGGGGCCCCCTCCACTCTTTTCCACAGGGTTTAACAGGGGGG; GCGGCCCCCCTCTTTTCCTCTTCCCCCCCACCCAGCGGGGGTTGCCGGGGACCCCCAACT; ACTATTTTTTTTTCAAAAAAAAACTCACCTCGGCCCCCCTCCCCCTCTCATACCACGTCT; TACAAACCCACACCCAACCACAACGTCTCTTCTCCTTTCCTCCCCTCGCCGTGGGGGGTG; GGGGGGGTTGTTACAGAGTGTCCCGTCGCGCTTTTGTTTTATCTTCTCCCACTCCTCTTT; TTCTAGCTTCGCTTCTTTTTTCACATACACTATCCCCTCCCTTTTACCTCCCCGTATCCC; ATTCTCTCCTTTCCCTTTCCCCTACTATGTTCCGTCGCCTCCACCCCCCTTCCTTTCCAC; TCTCCTTCCCTCTACTTCTACATCCACCTCCCTCCTCCCTCCTCCACCGCTCGCACCCCC; CGCTCCCTCCCTTTCTTCTCCTTCTTTTCATTTATCACATTAAATCCCATACAAGTAATT; AACATGTTTACCTACTTTAGCCATGCTTTCTTACAATACTCCTTCTTTCTTTCTCCCCAT; CCTCCAAACCCCCCCAACCCACCTCTAATGTGAATAATAATCGCGCCACACGCGCATAAT; TGTCAAGATGCGTCCCAAGCCAACACCCACGACTACCAAACAAACA;
• Restriction Sites: NotI-NotI
• ACCN: NM_003313
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003313.2, NP_003304.1
• RefSeq Size: 1312 bp
• RefSeq ORF: 966 bp
• Locus ID: 7264
• UniProt ID: Q13630
• Cytogenetics: 8q24.3
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Summary: Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream translation start site compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.