ACOX3 (NM_003501) Human Untagged Clone

SKU SC117938

ACOX3 (untagged)-Human acyl-CoA oxidase 3, pristanoyl (ACOX3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ACOX3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117938 sequence for NM_003501 edited (data generated by NextGen Sequencing); ATGGCATCCACTGTGGAAGGAGGCGACACAGCTCTGCTCCCAGAATTCCCCAGGGGGCCC; CTCGATGCCTACCGAGCAAGAGCGTCCTTCAGCTGGAAGGAGCTGGCGCTGTTCACGGAA; GGGGAGGGCATGCTCCGCTTTAAGAAAACCATCTTCTCAGCTCTTGAGAATGACCCTCTT; TTCGCTCGTTCCCCTGGAGCCGATCTGTCCTTGGAGAAGTATCGCGAGCTGAACTTCCTT; CGATGCAAGCGGATCTTCGAGTATGACTTCCTCAGTGTCGAAGACATGTTCAAGAGCCCT; CTGAAGGTCCCCGCCTTGATTCAGTGCCTGGGCATGTATGACTCTTCTCTGGCTGCCAAG; TACCTCCTCCATAGCTTGGTTTTTGGATCAGCAGTTTACAGTTCTGGTTCTGAAAGACAT; CTCACATATATTCAAAAGATCTTCAGGATGGAGATTTTTGGATGTTTTGCTCTGACCGAA; TTAAGCCACGGCAGTAATACCAAGGCCATTCGCACAACTGCCCACTACGATCCTGCCACT; GAGGAATTCATCATACATTCCCCTGATTTCGAAGCTGCCAAGTTTTGGGTTGGCAACATG; GGCAAGACAGCCACTCACGCGGTGGTGTTTGCTAAGCTGTGTGTGCCAGGGGACCAGTGC; CATGGGCTGCATCCCTTTATCGTGCAGATCCGGGACCCGAAGACCCTTCTTCCCATGCCT; GGAGTGATGGTTGGCGACATAGGAAAAAAACTCGGGCAGAACGGTCTGGATAATGGTTTC; GCCATGTTCCACAAGGTCAGAGTTCCTCGCCAGAGCCTTCTGAACCGGATGGGAGACGTC; ACCCCCGAGGGCACCTATGTCAGCCCCTTTAAGGACGTCAGGCAGCGCTTTGGAGCGTCC; CTGGGGAGCCTGTCCTCGGGCCGGGTCTCCATCGTGAGCCTGGCCATCCTTAACCTAAAG; CTGGCCGTGGCCATCGCTCTTCGCTTCTCAGCCACTCGGCGTCAGTTTGGACCCACAGAG; GAGGAGGAAATACCAGTGCTTGAGTATCCAATGCAGCAATGGCGCTTGCTTCCATATCTG; GCAGCTGTCTACGCCTTAGACCATTTCTCCAAGTCGCTCTTCCTGGACCTGGTGGAGCTC; CAGCGAGGACTTGCATCGGGAGACCGCAGCGCCAGACAGGCAGAGCTTGGACGTGAGATC; CACGCCCTGGCATCGGCCAGCAAGCCCCTGGCCTCGTGGACCACCCAGCAAGGAATTCAG; GAATGCCGGGAGGCGTGTGGAGGACACGGCTATCTGGCCATGAACCGGTTGGGTGTCCTT; AGAGATGACAACGATCCCAACTGCACATACGAAGGTGACAACAACATCCTGCTGCAGCAG; ACAAGCAACTATTTGCTGGGTCTCCTGGCACACCAGGTCCACGATGGAGCTTGCTTCCGC; AGTCCGCTGAAGTCAGTGGACTTTCTGGACGCCTATCCCGGCATCCTTGACCAGAAGTTT; GAGGTCTCCAGTGTTGCCGACTGCTTGGACTCTGCAGTCGCCCTGGCAGCATACAAGTGG; CTGGTTTGCTACCTGCTCCGAGAGACTTATCAAAAATTAAACCAAGAGAAAAGATCAGGA; AGCAGTGACTTTGAAGCAAGGAACAAATGCCAGGTGTCCCACGGCCGTCCGTTGGCGCTG; GCCTTCGTGGAGCTCACGGTGGTCCAGAGGTTCCACGAGCACGTGCACCAGCCTTCCGTG; CCGCCCTCGCTGCGGGCCGTGCTGGGGCGGCTCAGTGCTCTGTACGCCCTGTGGTCCCTG; AGCCGCCACGCGGCCCTGCTCTACCGAGGAGGATACTTCTCCGGTGAGCAGGCGGGAGAA; GTGTTGGAGAGCGCCGTCCTGGCTTTGTGTTCCCAGCTGAAAGACGATGCAGTTGCCCTG; GTAGACGTGATCGCTCCTCCTGACTTTGTTCTGGACTCACCGATTGGCAGAGCCGACGGC; GAGCTCTACAAAAACCTCTGGGGCGCTGTCCTGCAGGAAAGCAAGGTGTTGGAGCGGGCA; TCCTGGTGGCCAGAGTTTTCTGTGAACAAACCTGTCATAGGAAGTCTGAAATCGAAGCTC; TAG; ; Clone variation with respect to NM_003501.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003501 unedited; TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGACAGGCATCC; CCTGAGCGCCTGCTGGGCTCTTATCACGCGATGGCATCCACTGTGGAAGGAGGCGACACA; GCTCTGCTCCCAGAATTCCCCAGGGGGCCCCTCGATGCCTACCGAGCAAGAGCGTCCTTC; AGCTGGAAGGAGCTGGCGCTGTTCACGGAAGGGGAGGGCATGCTCCGCTTTAAGAAAACC; ATCTTCTCAGCTCTTGAGAATGACCCTCTTTTCGCTCGTTCCCCTGGAGCCGATCTGTCC; TTGGAGAAGTATCGCGAGCTGAACTTCCTTCGATGCAAGCGGATCTTCGAGTATGACTTC; CTCAGTGTCGAAGACATGTTCAAGAGCCCTCTGAAGGTCCCCGCCTTGATTCAGTGCCTG; GGCATGTATGACTCTTCTCTGGCTGCCAAGTACCTCCTCCATAGCTTGGTTTTTGGATCA; GCAGTTTACAGTTCTGGTTCTGAAAGACATCTCACATATATTCAAAAGATCTTCAGGATG; GAGATTTTTGGATGTTTTGCTCTGACCGAATTAAGCCACGGCAGTAATACCAAGGCCATT; CGCACAACTGCCCACTACGATCCTGCCACTGAGGAATTCATCATACATTCCCCTGATTTC; GAAGCTGCCAAGTTTTGGGGTTGGCAACATGGGCAAGACAGCCACTCACGCGGTGGTNGT; TGCTAAGCTGTGTGTGCAGGGGACCAGTGCCATGGGCTGCATCCCTTTATCGTGCAGATC; CGGGACCCCGAGACCCTTCTTCCCTGCCTGGAGTGATGGTTGGCGACATNAGAAAAAAAC; TCGGCAGAACGGTCTGGNATATGTTTCCCCCATGTCCACCGGTCANAGCTCCTGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003501 unedited; AGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTCCTCCTTAAGTCTTAAACCACGACATTC; AGATGATCTATGTGCAAATTAATAAGGTTATGACATAATTCAATTATGTGACAAAGCCGA; GGGAGAGTTGACAAAGAACCAGACTGGCATCATTGACTTCTCAATGAAGTGGAGCTCGGT; AAAAAATCTAAATGAATCACAGGTTGTCTGACCAAAAAATCCCTGACAATGGGCTGTTTA; CTTTAGAGAAAGGAGCTGAACCCTGAAAACTGAATGTGCGAAATTCTAATTATCAAAAAA; CCCACGGCGCATCAGGTAGACATGATCATCTGCATTTCTTTTCCACGCTGCAAATCACCG; CGATCCCAGATTATTCCAGCCGGCCGGGTGCCTCCCTCCCGTCCGCCTGGGCAGTTGAGG; CCAATCACCAGTTTAGGCCCACAGTTGCGGGCTCAAAAAGCAGCACCAATCTCCGGCGGT; GTCTGGAATTACAAATTTGAGGTCCCACGTCTGATAAGTTCGCTTCGTTTAATTAAACTT; GGCTGAATTGGGGCCACTCCACCTAAGCTTGATTTCCCACTTTTTAAACCCGGTTGTCAC; AAAAACCTCTGCCCACCGGATGCCCCTACACCCCTTGTTTTCTGCTGGCACCCCCTCTTG; GTTTTCTACTACCTCCTCTTCGCCCGCCCCCCCCTGCCGTCTTAAATATCCCGCCGAGGT; CCCCCCTCTTTTTCCTTCGTCCTTCCCCCCCCCTCCTCTTGCCCAACCGCGGATCCCCAT; CTGCCCACGTTTATNTGTTCCGTCCCCCTCTCCCCATTCTCTTTCTTTTTTCCCTATTTA; AACGATCCGCCCCCCGCTTTCCCTCCGCTTCCAACGGAAGAGGTGGATGGGTTTTTCTTT; CTCTTCCCTTTCACTTATACCGGNGGGCCGGCAGAGGGCCCCCCCCTGCGGCGCCCCGCC; CTATCGGACCCCCCCCTTTCATCCGTCCTCCATTTCTCCACCACCTTTCCTCCACGCCGC; CCTTCTTCCCTTTCCCCCGTCCCTCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_003501
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003501.1, NP_003492.1
• RefSeq Size: 2415 bp
• RefSeq ORF: 2103 bp
• Locus ID: 8310
• UniProt ID: O15254
• Cytogenetics: 4p16.1
• Domains: ACOX, Acyl-CoA_dh
• Protein Pathways: alpha-Linolenic acid metabolism, Biosynthesis of unsaturated fatty acids, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• Summary: Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3)is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes. Unlike the rat homolog, the human gene is expressed in very low amounts in liver such that its mRNA was undetectable by routine Northern-blot analysis or its product by immunoblotting or by enzyme activity measurements. However the human cDNA encoding a 700 amino acid protein with a peroxisomal targeting C-terminal tripeptide S-K-L was isolated and is thought to be expressed under special conditions such as specific developmental stages or in a tissue specific manner in tissues that have not yet been examined. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).